ZenComplete™: Metabolic Disease Contracts
ZenBio offers a variety of assays to test compounds as potential diabetes or obesity therapies. Customers can choose from any of the following assays or we can design a custom program tailored to each client's specific research needs.
- Adiponectin Secretion
The development of non-insulin dependent (Type 2) diabetes may involve dysregulation of adiponectin secretion. Supporting the link between obesity and Type 2 diabetes, decreased adiponectin secretion correlates with insulin resistance.
- Glucose Uptake
Diabetes is a prevalent metabolic disease affecting more than 25 million Americans. About 90% to 95% of patients have non-insulin dependent diabetes mellitus (NIDDM) or type II diabetes.
- Hepatic Glucose Production Assay
Compounds that increase the sensitivity of adipose to insulin may be useful in the treatment of diabetes and its complications
- Human Inhibition of Lipid Accumulation
The lipid accumulation assays provide the tools to study the compounds that inhibit cultured human adipocyte differentiation or lipogenesis.
- Human Islet Assays
The global prevalence of diabetes mellitus has reached epidemic proportions and is projected to continue to rise for the foreseeable future. It is estimated that diabetes will be the 7th leading cause of death worldwide by 2030. Both major forms of diabetes mellitus, type 1 (T1D) and type 2 (T2D), are ultimately caused by a failure of pancreatic β-cells to deliver insulin at a rate sufficient to account for metabolic demand..
- Human Lipid Accumulation
Lipid accumulation assays provide the tools to study the compounds that stimulate cultured human adipocyte differentiation or lipogenesis.
- Human Lipolysis
Lipolysis plays a central role in the regulation of energy balance.
Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids.
- Mitchondrial Mass Assay
ZenBio offers a cell-based high throughput assay to determine mitochondrial function by measuring mitochondrial mass using High-content image analysis. Cell-based analysis is generated from fluorescently labeled mitochondria using primary cells or cell lines treated with MitoTracker dyes.
- Oxygen Consumption Rate Analysis
ZenBio offers a cell-based high throughput assay to determine mitochondrial function by measuring oxygen consumption rate (OCR). The assay is performed using the MitoXpress® Xtra technology developed by Luxcel® Biosciences.
- Serum / Plasma Fatty Acid and Glycerol Detection Assay
However, an excess of circulating fatty acid over energy needs may contribute to hyperlipidemia, which is a risk factor for cardiovascular disease.
ZenBio is committed to customer service and technical support. We strive to meet the needs of all our clients. Listed below are some examples of recent custom work. If you do not see what you are looking for please contact a technical specialist at (919) 547-0692 or by email.
A Pdx-1-regulated soluble factor activates rat and human islet cell proliferation
Heather L. Hayes, Lu Zhang, Thomas C. Becker, Jonathan Haldeman, Samuel B. Stephens, Michelle Arlotto, Larry G. Moss, Christopher B. Newgard and Hans E. Hohmeier
Toxicological Risk Assessment–Proposed Assay Platform Using Stem and Progenitor Cell Differentiation in Response to Environmental Toxicants
John W. Ludlow, Alexander Kinev, Michael VanKanegan, Ben Buehrer, Nick Trotta and Joydeep Basu
Identification of a botanical inhibitor of intestinal diacylglyceride acyltransferase 1 activity via in vitro screening and a parallel, randomized, blinded, placebo-controlled ...
Rodney A. Velliquette1, Kerry Grann, Stephen R. Missler, Jennifer Patterson, Chun Hu, Kevin W. Gellenbeck, Jeffrey D. Scholten and R. Keith Randolph
Nutrition & Metabolism 2015, 12:27 doi:10.1186/s12986-015-0025-2
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