Peripheral Blood Mononuclear Cells (PBMCs)

Human PBMCs 200x

Cryopreserved Human Peripheral Blood Mononuclear Cells (PBMCs) are available as positive and negative controls for T-cell monitoring in ELISPOT, ELISA, cytokine bead array, tetramer/pentamer, and flow cytometry assays. A peripheral blood mononuclear cell (PBMC) is defined as any blood cell with a round nucleus (i.e. a lymphocyte, a monocyte, or a macrophage). These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of T cells, B cells and Natural Killer cells. These cells are often extracted from whole blood or from leukopacks using ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma.

Samples from each donor are tested via PCR to confirm non-reactivity.

Ordering Information:

Mononuclear Cells

Item#Item DescU/MPrice
SER-PBMC-FHuman Normal Peripheral Blood Mononuclear Cells (PBMCs), Cryopreserved, 15 million cells/vialVial$119.00
SER-PBMCHuman Normal Peripheral Blood Mononuclear Cells (PBMCs), FRESH, 15 million cells/vial, (Collect/Ship same day*, Testing Pending-requires signed waiver on file-contact us for more details)Vial$148.00
SER-PBMC-200-FHuman Normal Peripheral Blood Mononuclear Cells (PBMCs), Cryopreserved, 100 million cells/vialVial$337.00
SER-PBMC-200Human Normal Peripheral Blood Mononuclear Cells, FRESH (PBMCs), 200 million cells/vial, (Collect/Ship same day*, Testing Pending-requires signed waiver on file-contact us for more details)Vial$347.00
SER-D-PBMC-FHuman, TYPE 2 DIABETIC, Peripheral Blood Mononuclear Cells (PBMCs), 15 million cells/vialVial$163.00
SER-CD19-FHuman Normal Peripheral Blood B-Cells, CD19 , Cryopreserved, 1 million cells/vialVial$163.00
SER-PBCD56 NK-FHuman Normal Peripheral Blood Cryopreserved Natural Killer Cells (NK), CD56 , 5 million cells/vialVial$591.00
SER-PBCD56-UNT-FNormal Human Peripheral Blood Natural Killer Cells, CD56 , UNTOUCHED, Cryopreserved, 5 million cells/vial.+ Isolated by Negative Selection, indirect immunomagnetic NK cell labeling to deplete the non-CD56 NK cellsVial$863.00
SER-PMNHuman Normal Polymorphonuclear Cells (PMN), Cryopreserved, 15 million cells/vialVial$136.00
SER-PMN-CUSTOM-FHuman Polymorphonuclear Cells (PMN), Cryopreserved, 15 million cells/vial, Diseased State Donor (priced per disease)VialContact Us
LYMPH-1Lymphocyte Medium for Thawing, Plating and short-term maintenance [for use with all blood derived cells]100ml$94.00
LYMPH-1-50Lymphocyte Medium for Thawing, Plating and short-term maintenance [for use with all blood derived cells]50ml$55.00
LYMPH-100Lymphocyte Cryopreservation Medium, 100ml100ml$167.00
SER-PBMC-CUSTOM-FHuman Peripheral Blood Mononuclear Cells, Cryopreserved, Custom DISEASED Donor Collection. *Inquire about available samples*VialInquire

Lymphoid Cells

Item#Item DescU/MPrice
SER-CD3-FNormal Human Peripheral Blood T-Cells, CD3+ (Positive Selection), Cryopreserved, 5 million cells/vialVial$347.00
SER-CD3-PANT-FNormal Human Peripheral Blood Pan T-Cells, CD3+ (Negative Selection), Cryopreserved, 20 million cells/vialVial$722.00
SER-CD4 T-FNormal Human Peripheral Blood T-Cells, CD4 , Cryopreserved, 5 million cells/vialVial$347.00
SER-PBCD4 TH-N-FNormal Human Peripheral Blood Cryopreserved Naive T Helper Cells, CD4+ , 1 million cells/vialVial$347.00
SER-CBCD4 T-FNormal Human Cord Blood Cryopreserved CD4+ T Cells, 5 million cells/vialVial$428.00
SER-CBCD4+ TH-N-FNormal Human Cord Blood Cryopreserved NAIVE CD4+ T Helper Cells, 1 million cells/vialVial$461.00
SER-PBCD8 KT-N-FNormal Human Peripheral Blood Cryopreserved Naive T Killer Cells, CD8+ , 1 million cells/vialVial$705.00
SER-PBCD8 KT-FNormal Human Peripheral Blood Cryopreserved T Killer Cells, CD8+ , 5 million cells/vialVial$705.00
SER-PBCD25 TR-FNormal Human Peripheral Blood, Cryopreserved, CD25+ Regulatory T Cells, 2 millions cells/vialVial$862.00

Myeloid Cells

Item#Item DescU/MPrice
SER-CD14-FNormal Human Peripheral Blood MONOCYTES, CD14+ , Cryopreserved, 5 million cells/vialVial$347.00
SER-PBCD1c-FNormal Human Peripheral Blood Cryopreserved Myeloid Dendritic Cells, CD1c (BDCA-1) Positive: 500,000 cells/vialVial$866.00

*Eosinophils and Basophils available upon request

Recent Publications: View
Utilization of recombinant albumins in the expansion of human T lymphocytes
Atherly Pennybaker and Randall Alfano
DOI: 10.18609/cgti.2018.024
download pdf
Genetic Variant Q63R of Cannabinoid Receptor 2 (CB2R) Causes Differential ERK Phosphorylation in Human Immune Cells
Wang Jingru, Xu Juehua, Liu Jie, Zhu Huang, Peng Yanyan, Ding Zhi-Ming, and Hua Haiqing
download pdf
Multi-omics analysis reveals neoantigen-independent immune cell infiltration in copy-number driven cancers
Daniel J. McGrail, Lorenzo Federico, Yongsheng Li, Hui Dai, Yiling Lu, Gordon B. Mills, Song Yi, Shiaw-Yih Lin & Nidhi Sahni
download pdf
Functional Studies of Missense TREM2 Mutations in Human Stem Cell-Derived Microglia
Philip W. Brownjohn, James Smith, Ravi Solanki, Ebba Lohmann, Henry Houlden, John Hardy, Sabine Dietmann, and Frederick J. Livesey
Transcription factor specificity protein 1 modulates TGFβ1/Smad signaling to negatively regulate SIGIRR expression by human M1 macrophages stimulated with ...
Rui Yamaguchia, Arisa Sakamotoa, Reona Yamaguchib, Misa Haraguchia, Shinji Naraharaa, Hiroyuki Sugiuchia, Yasuo Yamaguchia
download pdf
Development and Evaluation of Tri-Functional Immunoliposomes for the Treatment of HER2 Positive Breast Cancer
Vaidya, T., Straubinger, R.M. & Ait-Oudhia, S. Pharm Res (2018) 35: 95.
download pdf
Regenerative potential of tonsil mesenchymal stem cells on surgical cutaneous defect
Sung-Chan Shin, Yoojin Seo, Hee Young Park, Da-Woon Jung, Tae-Hoon Shin, Haejin Son, Young Keum Kim, Jin-Choon Lee, Eui-Suk Sung, Jeon Yeob Jang, Hyung-Sik Kim & Byung-Joo Lee
Cell Death & Diseasevolume 9, Article number: 183 (2018), doi:10.1038/s41419-017-0248-4
download pdf
Inhibitory Effects of Toll-Like Receptor 4, NLRP3 Inflammasome, and Interleukin-1β on White Adipocyte Browning
Meshail Okla, Walid Zaher, Musaad Alfayez, Soonkyu Chung
download pdf
Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation Ioana I. Nitulescu, Sara C. Meyer, Qiang Jeremy Wen, John D. Crispino, Madeleine E. Lemieux, Ross L. Levine, Henry E. Pelish, Matthew D. Shair

download pdf
Differential regulation of IL-23 production in M1 macrophages by TIR8/SIGIRR through TLR4-or TLR7/8-mediated signaling RuiYamaguchi, ArisaSakamoto, TakatoshiYamamoto, ShinjiNarahara, YasuoYamaguchi
download pdf
Preclinical trial of the multi-targeted lenvatinib in combination with cellular immunotherapy for treatment of renal cell carcinoma Chengkuan Cai Jingyuan Tang Baixin Shen Liucheng Ding Yunpeng Shao Zhengsen Chen Yinchao Ma Haoliang Xue Zhongqing Wei
download pdf
Activated macrophages control human adipocyte mitochondrial bioenergetics via secreted factors Michaela Keuper, Stephan Sachs, Ellen Walheim, Lucia Berti, Bernhard Raedle, Daniel Tews, Pamela Fischer-Posovszky, Martin Wabitsch, Martin Hrabe de Angelis, Gabi Kastenmüller, Matthias H. Tschöp, Martin Jastroch, Harald Staiger, Susanna M. Hofmann
download pdf
Di-(2-ethylhexyl) phthalate suppresses IL-12p40 production by GM-CSF-dependent macrophages via the PPARα/TNFAIP3/TRAF6 axis after lipopolysaccharide ...R Yamaguchi, A Sakamoto, T Yamamoto, S Narahara, H Sugiuchi, A Hisada, T Katoh, Y Yamaguchi
DOI: a href="">
download pdf
Supplementary Information for High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients Yin Tanga, Zhuo Wanga, Ziming Lib, Jungwoo Kimc,d , Yuliang Denga , Yan Lie , James R. Heath , Wei Weic, Shun Lub,Qihui Shia download pdf
Evaluation of the Potency, Neutralizing Antibody Response, and Stability of a Recombinant Fusion Protein Vaccine for Streptococcus pyogenes E. BurletH. HogenEschA. DunhamG. Morefield
DOI: 10.1208/s12248-017-0069-5
download pdf
Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction Juan Tang, Yujun Shen, Guilin Chen, Qiangyou Wan, Kai Wang, Jian Zhang, Jing Qin, Guizhu Liu, Shengkai Zuo, Bo Tao, Yu Yu, Junwen Wang, Michael Lazarus & Ying Yu
download pdf
BCA2/RABRING7 Interferes with HIV-1 Proviral Transcription by Enhancing the SUMOylation of IκBα Marta Colomer-Llucha and Ruth Serra-Moreno
J. Virol. doi:10.1128/JVI.02098-16
download pdf
Targeting IRE1 with Small Molecules Counteracts Progression of Atherosclerosis Ozlem Tufanlia,, Pelin Telkoparan Akillilara, Diego Acosta-Alvearc, Begum Kocaturka, Umut I. Onata, Syed M. Hamida,, Ismail Cimena, Peter Walter, Christian Weberd,, Ebru Erbay download pdf
Matrix Stiffness Exerts Biphasic Control Over Monocyte-Endothelial Adhesion via Rho-mediated ICAM-1 Clustering Harry A Scott, Boi Quach, Xiao Yang, Soroush Ardekani, Andrea Paulina Cabrera, Randall Wilson, Ilhem Messaoudi-Power and Kaustabh Ghosh
Integr. Biol., 2016, Accepted Manuscript
DOI: 10.1039/C6IB00084C
download pdf
Lentivirus pre-packed with Cas9 protein for safer gene editing J G Choi, Y Dang, S Abraham, H Ma, J Zhang, H Guo, Y Cai, J G Mikkelsen, H Wu, P Shankar and N Manjunath
Gene Therapy , (7 April 2016) | doi:10.1038/gt.2016.27
download pdf
Neutrophil elastase enhances IL-12p40 production by lipopolysaccharide-stimulated macrophages via transactivation of the PAR-2/EGFR/TLR4 signaling pathway Rui Yamaguchia, b, Takatoshi Yamamotoa, Arisa Sakamotoa, Shinji Naraharaa, Hiroyuki Sugiuchia, Yasuo Yamaguchi
download pdf
Inactivation of the miR-183/96/182 Cluster Decreases the Severity of Pseudomonas aeruginosa-Induced KeratitismiR-183/96/182 in P. aeruginosa-Induced Keratitis Chithra K. Muraleedharan; Sharon A. McClellan; Ronald P. Barrett; Cui Li; Daniel Montenegro; Thomas Carion; Elizabeth Berger; Linda D. Hazlett; Shunbin Xu
Investigative Ophthalmology & Visual Science April 2016, Vol.57, 1506-1517. doi:10.1167/iovs.16-19134
download pdf
Macrophage PPAR-γ and Impaired Wound Healing in Type 2 Diabetes Rita E. Mirza, Milie M. Fang, Margaret L. Novak, Norifumi Urao, Audrey Sui, William J. Ennis andTimothy J. Koh
Journal of Pathology, DOI: 10.1002/path.4548
download pdf
Repositioning Potential of PAK4 to Osteoclastic Bone Resorption† Sik-Won Choi1,‡, Jeong-Tae Yeon2,‡, Byung Jun Ryu1, Kwang-Jin Kim2, Seong-Hee Moon1, Hyuk Lee3, Myeung Su Lee4, Sam Youn Lee5, Jin-Chul Heo6, Sang-Joon Park7 andSeong Hwan Kim1,* DOI: 10.1002/jbmr.2468