Subcutaneous Preadipocyte and Adipocyte Media
All ZenBio media products are ready-to-use when purchased. No mixing of growth factors or antibiotics is necessary. All media products are available as phenol-red free, serum-free, or specifically customized to meet your research needs.
Subcutaneous basal medium
Basal medium is serum-free and contains essential additives to maintain subcutaneous cells for up to 3 days. It can be supplemented for specific research purposes. The medium is available in 500ml bottles and is ready-to-use upon arrival.
Subcutaneous preadipocyte medium
Preadipocyte medium is used to plate preadipocytes prior to differentiation and contains growth factors necessary for expansion of subcutaneous preadipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.
Subcutaneous differentiation medium
Differentiation medium is used to differentiate subcutaneous preadipocytes to mature adipocytes in culture. The medium is available in 100ml or 500ml bottles and is ready-to-use upon arrival.
Subcutaneous adipocyte medium
Adipocyte medium is used as a maintenance medium after differentiation and contains all of the factors necessary to support lipid accumulation in the maturing adipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.
Subcutaneous preadipocytes cryopreservation medium
Cryopreservation medium is used for long-term cryopreservation of subcutaneous preadipocytes. This medium has been specifically formulated for optimized recovery of human preadipocytes after thawing. It is available in 100ml bottles.
Order Information
Subcutaneous Media
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| BM-1 | Subcutaneous Basal Medium | 500 ml | $125.00 |
| PM-1 | Subcutaneous Preadipocyte Medium | 500 ml | $136.00 |
| DM-2 | Subcutaneous Preadipocyte Differentiation Medium | 100ml | $145.00 |
| DM-2-500 | Subcutaneous Preadipocyte Differentiation Medium | 500ml | $626.00 |
| AM-1 | Subcutaneous Adipocyte Maintenance Medium | 500ml | $136.00 |
| FM-1-100 | Preadipocyte Cryopreservation medium | 100ml | $242.00 |
| KT-02 | Subcutaneous Adipocyte Differentiation Kit: sufficient media to differentiate 1 96 well plate human subcutaneous preadipocytes to adipocytes. 50ml PM-1, 25ml DM-2, 25ml AM-1, 1 blank sterile 96 well plate | KIT | $98.00 |
If you require special media modifications, we offer a Custom Media Service starting at one bottle per order. Please contact us for more details or consult our Price List (.pdf). Examples of some common modifications include: Phenol-Red free, Serum-free, Dexamethasone-free, Insulin-free, PPAR Gamma Agonist-free and Antibiotic-free.
Subcutaneous Preadipocyte and Adipocyte Media Publications
Upscaling of pneumatic membrane valves for the integration of 3D cell cultures on chip
Nina Compera, Scott Atwell, Johannes Wirth, Bernhard Wolfrum and Matthias MeierDOI: 10.1039/D1LC00194A
Physicochemical and functional characterization of MYL-1501D, a proposed biosimilar to insulin glargine
Parag Goyal, Harish Venkatraman Pai, Phanichand Kodali, Bhavesh Vats, Navratna Vajpai, Shankara Annegowda, Krishnappa Mane, Shamini Mohan, Shruti Saxena, Anil Bangalore Veerabhadraia, Milee Palande, Preethy Sasankan Nair, Digvijay Chandrashekar More, Umamaheshwara Rao Karudumpa, Kunala Jyothirmai, Adroha Bhattacharya, Frida Almeida, Santosh Gulab Khyade, Shankara Gouda, Daniel J. Ranayhossaini, Praveen Reddy Moole, Jeffrey P. Smith, Abhijit Barve, Ramakrishnan Melarkode, Rajesh UllanatDOI: https://doi.org/10.1371/journal.pone.0253168
GRIM19 Impedes Obesity by Regulating Inflammatory White Fat Browning and Promoting Th17/Treg Balance
JooYeon Jhun, Jin Seok Woo, Seung Hoon Lee, Jeong-Hee Jeong, KyungAh Jung, Wonhee Hur, Seon-Yeong Lee, Jae Yoon Ryu, Young-Mee Moon, Yoon Ju Jung, Kyo Young Song, Kiyuk Chang, Seung Kew Yoon, Sung-Hwan Park and Mi-La ChoCells 2021, 10, 162.
https://doi.org/10.3390/cells10010162
Vanadium-protein complex inhibits human adipocyte differentiation through the activation of β-catenin and LKB1/AMPK signaling pathway
Shuang Zhang, Lei Yan, Sang Moo Kimhttps://doi.org/10.1371/journal.pone.0239547
A novel small molecule A2A receptor agonist, indirubin-3'-monoxime, alleviates lipid-induced inflammation and insulin resistance in 3T3-L1 adipocytes
Saynaz A. Choudhary, Nikita Bora, Dipanjan Banerjee, Leena Arora, Anindhya Sundar Das, Rakesh Yadav, Karl-Norbert Klotz, Durba Pal, Anupam Nath Jha, Suman DasguptaDOI: 10.1042/BCJ20190251
Microphysiological Engineering of Self-Assembled and Perfusable Microvascular Beds for the Production of Vascularized Three-Dimensional Human Microtissues
Jungwook Paek, Sunghee E. Park, Qiaozhi Lu, Kyu-Tae Park, Minseon Cho, Jeong Min Oh, Keon Woo Kwon, Yoon-suk Yi, Joseph W. Song, Hailey I. Edelstein, Jeff Ishibashi, Wenli Yang, Jacob W. Myerson, Raisa Y. Kiseleva, Pavel Aprelev, Elizabeth D. Hood, Dwight Stambolian, Patrick Seale, Vladimir R. Muzykantov, Dongeun Huhhttps://doi.org/10.1021/acsnano.9b00686
Pseudomonas aeruginosa-derived pyocyanin reduces adipocyte differentiation, body weight, and fat mass as mechanisms contributing to septic cachexia
Nika Larian, Mark Ensor, Sean E. Thatcher, Victoria English, Andrew J. Morris, Arnold Stromberg, Lisa A.Cassishttps://doi.org/10.1016/j.fct.2019.05.012
Genetic regulation of enoyl-CoA hydratase domain-containing 3 in adipose tissue determines insulin sensitivity in African Americans and Europeans
Neeraj K. Sharma, Chia-Chi Chuang Key, Mete Civelek, Martin Wabitsch, Mary E. Comeau, Carl D. Langefeld, John S. Parks, Swapan K. DasDiabetes 2019 Apr; db181229.
https://doi.org/10.2337/db18-1229
Mature Human White Adipocytes Cultured under Membranes Maintain Identity, Function, and Can Transdifferentiate into Brown-like Adipocytes
Matthew J.Harms, Qian Li, Sunjae Lee, Cheng Zhang, Bengt Kull, Stefan Hallen, Anders Thorell, Ida Alexandersson, Carolina E.Hagberg, Xiao-Rong Peng, Adil Mardinoglu, Kirsty L. Spalding, 5Jeremie Boucherhttps://doi.org/10.1016/j.celrep.2019.03.026
Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells
Michael D. West, Ching-Fang Chang, Dana LaroccaEmail author, Jie Li, Jianjie Jiang, Pamela Sim, Ivan Labat, Karen B. Chapman, Kari E. Wong, James Nicoll, Michael J. Van Kanegan, Aubrey D. N. J. de Grey, Igor O. Nasonkin, Andreas Stahl and Hal SternbergStem Cell Research & Therapy 201910:7
https://doi.org/10.1186/s13287-018-1087-7
Stabilization of lncRNA GAS5 by a small molecule and its implications in diabetic adipocytes
Yan Shi, Sajan Parag, Rekha Patel, Ashley Lui, Michel Murr, Jianfeng Cai, Niketa A.Patelhttps://doi.org/10.1016/j.chembiol.2018.11.012
Decreased TLR3 in Hyperplastic Adipose Tissue, Blood and Inflamed Adipocytes is Related to Metabolic Inflammation
Jéssica Latorre, José M. Moreno-Navarrete, Mónica Sabater, Maria Buxo, José I. Rodriguez-Hermosa, Jordi Girones, José M. Fort, Ramón Vilallonga, Wifredo Ricart, Rafael Sim, José M. Fernández-Real, Francisco J. OrtegaCell Physiol Biochem 2018;51:1051-1068 DOI: 10.1159/00049548
Polyurethane Scaffolds Seeded with Autologous Cells Can Regenerate Long Esophageal Gaps: an Esophageal Atresia Treatment Model
Todd Jensen, Heather Wanczyk, Ishna Sharma, Adam Mitchell, Wael N.Sayej, Christine Finckhttps://doi.org/10.1016/j.jpedsurg.2018.09.024
Notch signaling enhances stemness by regulating metabolic pathways through modifying p53, NF-kB, and HIF-1α
Dr. Hiroyuki Moriyama, Dr. Mariko Moriyama, Dr. Toshiyuki Ozawa, Prof. Daisuke Tsuruta, Dr. Taro Iguchi, Dr. Satoshi Tamada, Prof. Tatsuya Nakatani, Koichi Nakagawa, Dr. Takao Hayakawahttps://doi.org/10.1089/scd.2017.0260
Completely serum-free and chemically defined adipocyte development and maintenance
Ann-Cathrin Volz, Petra Juliane KlugerDOI: https://doi.org/10.1016/j.jcyt.2018.01.004
Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury
Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko, Cesar V. BorlonganDOI https://doi.org/10.1007/7651_2018_121
MicroRNA-221-3p regulates Angiopoietin-like 8 (ANGPTL8) expression in adipocytes
Raghavendra Mysore Francisco J Ortega Jéssica Latorre Maria AhonenHanna Savolainen-Peltonen Pamela Fischer-Posovszky Martin WabitschVesa M Olkkonen José M Fernández-Real P A Nidhina HaridasThe Journal of Clinical Endocrinology & Metabolism, jc.2017-00453, https://doi.org/10.1210/jc.2017-00453
Adipose tissue macrophages impair preadipocyte differentiation in humans
Li Fen Liu, Colleen M. Craig, Lorna L. Tolentino, Okmi Choi, John Morton, Homero Rivas, Samuel W. Cushman, Edgar G. Engleman, Tracey McLaughlinhttp://dx.doi.org/10.1371/journal.pone.0170728
EGF and hydrocortisone as critical factors for the co-culture of adipogenic differentiated ASCs and endothelial cells
Ann-Cathrin Volza, Birgit Huberb, Alina Maria Schwandtc, Petra Juliane Klugerhttp://dx.doi.org/10.1016/j.diff.2017.01.002
MALAT1 in human adipose stem cells modulates survival and alternative splicing of PKCδII in HT22 cells
Ghattas El Bassit, Rekha S. Patel, Gay Carter, Vyshakh Shibu, Achintya Patel, Shijie Song, Michel Murr, Denise R. Cooper, Paula C. Bickford and Niketa A. PatelDOI: 10.1210/en.2016-1819
Eicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects
L.M. Laiglesia, S. Lorente-Cebrián, P.L. Prieto-Hontoria1, M. Fernández-Galilea2, S.M.R. Ribeiro, N. Sáinz, J.A. Martínez, M.J. Moreno-AliagaDOI: http://dx.doi.org/10.1016/j.jnutbio.2016.07.019
Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation
Woonhyeok Jeong, Xiao Yang, Jeongmi Lee, Youngwook Ryoo, Jinhee Kim, Youngkee Oh, Sunyoung Kwon, Dalie Liu and Daegu SonStem Cell Research & Therapy20167:117
DOI: 10.1186/s13287-016-0378-0
DIFFERENT ANTI-ADIPOGENIC EFFECTS OF BIO-COMPOUNDS ON PRIMARY VISCERAL PRE-ADIPOCYTES AND ADIPOCYTES
Monica Colitti, Bruno Stefanonhttp://dx.doi.org/10.17179/excli2016-267
Screening for Identification of Personalized Food to Promote Adiponectin Secretion in Patients with Cancer
MIKI SAKAUE, KAZUHISA MAEDA, SATOSHI OHNO and TOSHINORI ITOAnticancer Research July 2016 vol. 36 no. 73673-3677
Prostaglandin E 2 Exerts Multiple Regulatory Actions on Human Obese Adipose Tissue Remodeling, Inflammation, Adaptive Thermogenesis and Lipolysis
Verónica GarcÃa-Alonso, Esther Titos, Jose Alcaraz-Quiles, Bibiana Rius, Aritz Lopategi, Cristina López-Vicario, Per-Johan Jakobsson, Salvadora Delgado, Juanjo Lozano, Joan CláriaPublished: April 28, 2016 http://dx.doi.org/10.1371/journal.pone.0153751
III. CHAPTER II: Ellagic acid inhibits adipocyte differentiation through coactivator associated arginine methyltransferase 1-mediated chromatin modification
Inhae Kanga,b, Meshail Oklaa,b, and Soonkyu ChungHypoxia alters the expression of dipeptidyl peptidase 4 (DPP4) and induces developmental remodeling of human preadipocytes
Helena H. Chowdhury, Jelena Velebit, Nataŝa Radić, Vito Franĉiĉ, Marko Kreft, Robert ZorecMechanisms of peroxisome proliferator activated receptor γ regulation by non-steroidal anti-inflammatory drugs
Ana C. Puhl, Flora A. Milton, Aleksandra Cvoro, Douglas H. Sieglaff, Jéssica C.L. Campos, Amanda Bernardes, Carly S. Filgueira, Jan Lammel Lindemann, Tuo Deng, Francisco A.R. Neves, Igor Polikarpov, and Paul WebbNucl Recept Signal. 2015; 13: e004
The Effects of Platelet-rich Plasma on Cell Proliferation and Adipogenic Potential of Adipose-derived Stem Cells
Dr. Han-Tsung Liao, Mr. Isaac B James, Prof. Kacey Marra, and Dr. J. Peter Rubin. Tissue Engineering Part A. -Not available-, ahead of print. doi:10.1089/ten.TEA.2015.0159Expression analysis of human adipose-derived stem cells during in vitro differentiation to an adipocyte lineage
Latha Satish, J. Michael Krill-Burger, Phillip H. Gallo, Shelley Des Etages, Fang Liu,Brian J. Philips, Sudheer Ravuri, Kacey G. Marra, William A. LaFramboise, Sandeep Kathju and J. Peter RubinBMC Medical Genomics 2015, 8:41 doi:10.1186/s12920-015-0119-8
Decelerating mature adipocyte dedifferentiation by media composition
Mrs. Birgit Huber and Dr. Petra J Kluger. Tissue Engineering Part C: Methods. doi:10.1089/ten.TEC.2015.0166.Expression analysis of human adipose-derived stem cells during in vitro differentiation to an adipocyte lineage
Latha Satish, J. Michael Krill-Burger, Phillip H. Gallo, Shelley Des Etages, Fang Liu, Brian J. Philips, Sudheer Ravuri, Kacey G. Marra, William A. LaFramboise, Sandeep Kathju and J. Peter RubinBMC Medical Genomics 2015, 8:41 doi:10.1186/s12920-015-0119-8
In Vitro Effects of Bisphenol A β-D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes
Jonathan G. Boucher, Adéle Boudreau, Shaimaa Ahmed, and Ella Atlashttp://dx.doi.org/10.1289/ehp.1409143Environmental Health Perspectives, May 2015
MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue
Tung-Yueh Chuang, Hsiao-Li Wu, Chen-Chun Chen, Gloria Mabel Gamboa, Lawrence C. Layman, Michael P. Diamond, Ricardo Azziz, and Yen-Hao ChenJournal of Diabetes Research Article ID 943659 (March 2015)
