Support: Kit Manuals

• Adipogenesis/Triglyceride Kits
• Cosmeceutical Kits
• Lipolysis Kits
• Protein Secretion Kits
• Serum/Plasma Fatty Acid and Glycerol Kits

Lipolysis Kits

• Cultured Human Adipocyte Lipolysis Assay Kit - Glycerol (Colorimetric) (LIP-1) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Lipolysis Assay Kit (Reagents Only) - Glycerol Detection 500 point kit (LIP-1RB)(pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Cultured Human Adipocyte Lipolysis Assay Kit - NEFA (LIP-2) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Alterations in lipolytic capacity have also been implicated in the susceptibility to obesity of African-American individuals versus their Caucasian cohorts (Danadian et al. 2001).
• Lipolysis Assay Kit (Reagents Only) - NEFA Detection 500 Point Kit (LIP-2RB) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Alterations in lipolytic capacity have also been implicated in the susceptibility to obesity of African-American individuals versus their Caucasian cohorts (Danadian et al. 2001).
• Cultured Human Adipocyte Lipolysis Assay Kit - Combo (LIP-3) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Cultured Human Adipocyte Lipolysis Assay Kit for Detection of Both Free Glycerol and Non-Esterified Fatty Acids (LIP-3-RB) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Alterations in lipolytic capacity have also been implicated in the susceptibility to obesity of African-American individuals versus their Caucasian cohorts (Danadian et al. 2001).
• 3T3-L1 with cells Adipocyte Lipolysis Kit - Glycerol (Colorimetric) (LIP-1-L1, LIP-1-L1-F, LIP-1-NCL1DIF) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyce ride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Lipolysis Assay Kit, Glycerol Detection, WITH cryopreserved vial subcutaneous preadipocytes (LIP-1-SPF) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Lipolysis Assay Kit, WITH cryopreserved vial omental preadipocytes (LIP-1-OPF) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corre sponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• Adipose Tissue Explant Lipolysis Assay Kit: Glycerol Detection (LIP-6-NC) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).
• 3T3-L1 with cells Adipocyte Lipolysis Assay Kit - NEFA (LIP-2-L1) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Alterations in lipolytic capacity have also been implicated in the susceptibility to obesity of African-American individuals versus their Caucasian cohorts (Danadian et al. 2001).
• 3T3-L1 with cells Adipocyte Lipolysis Assay Kit - Combo (LIP-3-L1) (pdf)
  Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).

Adipogenesis/Triglyceride Kits

• Human Adipocyte Differentiation kits
• Human Adipogenesis Assay Kit (DIF-AG) (pdf)
  The differentiation assay kits provide the tools to study the compounds that stimulate cultured human adipocyte differentiation or lipogenesis. Such compounds may be PPAR agonists or a combination of thiazolidinediones and glucocorticoids that are potentially useful in the treatment of diabetes.
• Cultured Human Adipocyte Differentiation Assay Kit - Glucocorticoid Analogues (DIF-GLUC) (pdf)
  The differentiation assay kits provide the tools to study the compounds that stimulate human adipocyte differentiation or lipogenesis. Such compounds may be PPAR agonists or a combination of thiazolidinediones and glucocorticoids that are potentially useful in the treatment of diabetes.
• Triglyceride Assay Kit (TG-1-NC) (pdf)
  The contents of this kit are sufficient for the assay of 100 assay points in a 96 well plate format. The only reagents sold separately are Glycerol Reagent A (cat# RGTA-10) and the glycerol standard for the Triglyceride Assay kit (cat# TG-GLYSTAN). For additional reagents or formats, please order our bulk 500 point assay kit (cat# TG-5RB).
• Triglyceride Assay Kit-Bulk 5 Plate Kit (TG-5-RB) (pdf)
  The contents of this kit are sufficient for the assay of up to five 96 well plates (500 assay points). The protocol is designed for assay of cells in a 96-well format. For other formats, please adjust the volumes added to each well according to the surface area of the well/flask you are using. See your cultureware manufacturer's technical information for the specifications.
• Lipid Staining Kit (ST-R100) (pdf)
  This protocol is designed to stain cells in a 96-well format using Oil Red O. Oil Red O is a fat-soluble diazo dye that stains neutral triglycerides and lipids. For other formats, please adjust the volumes added to each well according to the surface area of the well/flask you are using.
• 3T3-L1 Adipocyte Kit (KT01) (pdf)
  The 3T3-L1 Adipocyte Kit is designed to allow consistent differentiation of 3T3-L1 preadipocytes into mature adipocytes. The volumes listed are suitable for the differentiation of 3T3-L1 preadipocytes in a 96 well format. For other volumes, please order media individually (cat# PM-1-L1, 500ml; DM-2-L1 100ml; AM-1-L1 500ml).

Protein Secretion Kits

• Human Adiponectin ELISA Kit User Manual (ADIP-1, ADIP-2) (pdf)
  Obesity, and obesity-related disorders, are reaching alarming proportions in the US, and are on the increase in Europe and Asia. A deeper understanding of the molecular and cellular dynamics of such disorders, and their subsequent amelioration, will have a far-reaching impact on the quality of life of millions of people worldwide.

Serum/Plasma Fatty Acid and Glycerol Kits

• 96-well Serum/Plasma Fatty Acid Kit - 100 Point Kit (SFA-1) (pdf)
  This kit is designed to accurately determine the amount of free fatty acid present in blood, serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: sodium citrate, ammonium oxalate and EDTA. NOTE: Heparin or Heparinized tubes should not be used because this will generate inaccurate readings. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.
• 96-well Serum/Plasma Fatty Acid Kit - 500 Point Kit (SFA-5) (pdf)
  This kit is designed to accurately determine the amount of free fatty acid present in blood serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: sodium citrate, ammonium oxalate and EDTA. NOTE: Heparin or Heparinized tubes should not be used because this will generate inaccurate readings. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.
• 96-well Serum/Plasma Fatty Acid Kit - 1000 Point Kit (SFA-10) (pdf)
  This kit is designed to accurately determine the amount of free fatty acid present in blood serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: sodium citrate, ammonium oxalate and EDTA. NOTE: Heparin or Heparinized tubes should not be used because this will generate inaccurate readings. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.
• Serum/Plasma Glycerol and Fatty Acid Detection Kit (GFA-1) (pdf)
  This kit is designed to accurately determine the amount of free fatty acid and glycerol present in blood serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: sodium citrate, ammonium oxalate and EDTA. NOTE: Heparin or Heparinized tubes should not be used because this will generate inaccurate readings. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.
• 96-well Serum/Plasma Glycerol Detection Kit (SGA-1) (pdf)
  This kit is designed to accurately determine the amount of glycerol present in blood serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: sodium citrate, ammonium oxalate and EDTA. NOTE: Heparin or Heparinized tubes should not be used because this will generate inaccurate readings. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.
• Serum Triglyceride Assay Kit, 100-point, REAGENTS ONLY (STG-1NC) (pdf)
  This kit is designed to accurately determine the amount of triglyceride present in blood serum or plasma of humans, mice, rats, and other animals in a 96-well format for increased throughput analysis. Blood can be collected in plain evacuated tubes or in the presence of common anti-coagulants: heparin, EDTA, sodium citrate, or ammonium oxalate. Serum should be separated from clotted blood by centrifugation as soon as possible and may be stored frozen (-20°C) prior to analysis.

Cosmeceutical Kits

• AOX-2RB manual (pdf)
  The Zen-Bio ORAC (Oxygen Radical Absorbance Capacity) Antioxidant Assay Kit can be used to determine the total antioxidant capacity of biological fluids, cells, and tissue
• Cellulite Treatment Screening Lipolysis Kit - Glycerol (LIP-10) (pdf)
  This kit provides the tool to study chemical compounds that may influence lipolysis in cultured human adipocytes.This kit specifically measures the free glycerol released by the breakdown of triglyceride. The amount of free glycerol released from the cells is proportional to the ability of the test chemical to break down triglyceride
• Cellulite Treatment Screening Lipolysis Kit - NEFA (LIP-11) (pdf)
  This kit provides the tool to study chemical compounds that may influence lipolysis in cultured human adipocytes.This kit specifically measures the free glycerol released by the breakdown of triglyceride. The amount of free glycerol released from the cells is proportional to the ability of the test chemical to break down triglyceride
• Cellulite Treatment Screening Lipolysis Kit - Glycerol and NEFA (LIP-12) (pdf)
  This kit provides the tools to study chemical compounds that may influence lipolysis in cultured human adipocytes. This kit specifically measures both the non-esterified fatty acids (NEFA) and the glycerol released by the breakdown of triglyceride
• Wrinkle Treatment Screening Kit (DIF-10) (pdf)
  FAT AS YOUR FRIEND. The increased demand for anti-aging treatments has ranged from natural botanicals, cosmetics to surgical intervention (Majeed & Prakash 2002). In the battle against the appearance of aging, fat is the friend, not the foe. In addition to the treatment of facial wrinkles, the transfer of one's own fat can be used to treat indented acne scars, fill out the back of the hands and correct skin depressions (Christensen et al. 2005).
• ABTS Antioxidant Assay Kit (AOX-1) (pdf)
  The ZenBio ABTS Antioxidant Assay Kit measures the ability of a sample to inhibit ABTS•+ radical cation formation induced by metmyoglobin and hydrogen peroxide.
• ORAC Antioxidant Assay Kit (AOX-2) (pdf)
  The ZenBio ORAC Antioxidant Assay Kit measures the ability of a sample to inhibit peroxyl-radical oxidation of fluorescein.
• DPPH Antioxidant Assay Kit (AOX-3) (pdf)
  The ZenBio DPPH Antioxidant Assay Kit measures the reduction of the stable DPPH radical by a sample through electron transfer.
• Cu-TAC Antioxidant Assay Kit (AOX-4) (pdf)
  The ZenBio Cu-TAC Antioxidant Assay Kit measures the reduction of Copper(II) to Copper(I) in the presence of the aromatic chelator, neocuproine, and can be inhibited by uric acid or Trolox.
• TAC-BCS Antioxidant Assay Kit (AOX-5) (pdf)
  The ZenBio TAC-BCS Antioxidant Assay Kit measures the reduction of copper(II) to copper(I) in the presence of the aromatic chelator, bathocuproinedisulfonate (BCS) and can be inhibited by uric acid or Trolox.
• HORAC Antioxidant Assay Kit (AOX-6) (pdf)
  The ZenBio HORAC Antioxidant Assay Kit measures the ability of a sample to inhibit hydroxyl-radical oxidation of fluorescein.
• NORAC Antioxidant Assay Kit (AOX-7) (pdf)
  The ZenBio NORAC Antioxidant Assay Kit measures the ability of a sample to inhibit peroxynitrite oxidation of dihydrorhodamine 123.
• CLORAC Antioxidant Assay Kit (AOX-8) (pdf)
  The ZenBio CLORAC Antioxidant Assay Kit measures the ability of a sample to inhibit hypochlorite oxidation of fluorescein.
• CAA Antioxidant Assay Kit (AOX-9) (pdf)
  The ZenBio CAA Antioxidant Assay Kit provides the reagents to measure the capacity of samples to inhibit peroxyl-radical oxidation of DCFH in a cell-based system.
• FRAP Antioxidant Assay Kit (AOX-11) (pdf)
  The ZenBio FRAP Antioxidant Assay Kit measures the ability of a sample to prevent the reduction of Fe(III) to Fe(II) at low pH in the presence of a chelating probe, tripyridyltriazine (TPTZ).
• Activated ABTS Antioxidant Assay Kit (AOX-14) (pdf)
  The ZenBio Activated ABTS Antioxidant Assay Kit measures the ability of a sample to reduce the pre-formed ABTS•+ radical cation.
• Ferrous Iron Chelating (FIC) Assay Kit (AOX-15) (pdf)
  
• Cupric Ion Chelating (CIC) Assay Kit (AOX-16) (pdf)
  
• Total Phenolic Content Assay Kit (AOX-17) (pdf)