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Support: Cell Manuals
3T3L1 Adipocyte Care Manual (pdf) 3T3-L1 adipocytes have been fundamental in metabolic disease research for 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Ho ward Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the underst anding of basic cellular mechanisms associated with diabetes, obesity and related disorders.
Human Subcutaneous Adipocyte/Preadipocyte Care Manual (pdf) Cultured human adipocytes. The adipocyte precursor cells (preadipocytes) are isolated from subcutaneous adipose tissue of healthy non-diabetic donors between 18 and 60 years old undergoing elective surgery. The preadipocytes are isolated by centrifugal force after collagenase treatment. Preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. This instruction manual describes procedures t o induce human preadipocytes to differentiate into mature adipocytes as well as culturing methods for human preadipocytes and adipocytes. The process of differentiating preadipocytes to adipocytes has been patent protected by ZenBio under US patent number 6153432.
Visceral Adipocyte Care Manual (pdf) Cultured human visceral adipocytes Visceral preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. This instruction manual describes procedures required to induce human preadipocytes to differentiate into mature adipocytes as well as culturing methods for human preadipocytes and adipocytes. The process of differentiating preadipocytes to adipocytes has been patent protected by ZenBio under US patent number 6153432.
Cryopreserved Feline SQ Preadipocytes (FP-F) (pdf) Cultured feline adipocytes Feline preadipocytes can be cultured as growing precursor cells or differentiated into adipocytes using medium supplemented with adipogenic and lipogenic hormones. This instruction manual describes procedures required to induce feline preadipocytes to differentiate into mature adipocytes as well as culturing methods for feline preadipocytes and adipocytes. The process of differentiating preadipocytes to adipocytes has been patent protected by ZenBio under US patent number 6153432.
Human CD3+ T Cells (pdf) Cryopreserved CD3 +T cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon arrival. Orders are delivered via Federal Express courier.
Human CD4+ T Cells (pdf) T Cells have a key function in the adaptive immune system. They can either promote growth and differentiation of other immune cells or show suppressive function and down - regulate immune reactions.
Human Cryopreserved Activated CD8 T-Cells (pdf) CD8+ T cells are recognized as TC cells once they become activated and are generally classified as having a pre-defined cytotoxic role within the immune system. However, CD8+ T cells also have the ability to make some cytokines. Antigen presentation stimulates T cells to become either "cytotoxic" CD8+ cells or "helper" CD4+ cells. A cytotoxic T cell belongs to a sub-group of T lymphocytes that are capable of inducing the death of infected somatic or tumor cells; they kill cells that are infected with viruses (or other pathogens), or are otherwise damaged or dysfunctional. Most cytotoxic T cells express T-cell receptors ) that can recognize a specific antigenic peptide bound to Class I MHC molecules, present on all nucleated cells, and a glycoprotein called CD8, which is attracted to non-variable portions of the Class I MHC molecule. The affinity between CD8 and the MHC molecule keeps the TC cell and the target cell bound closely together during antigen-specific activation.
Human Cryopreserved CD14 Monocytes (pdf) Monocytes are circulating precursors of macrophages and dendritic cells that migrate from the blood stream across vascular endothelium for immunological surveillance, as well as respond to inflammation. They act as antigen-presenting immune cells and can phagocytize and degrade microbes and particulate matter. Monocytes can differentiate into several different cell types. The most common applications for Monocytes are the in vitro differentiation into monocyte-derived dendritic cells, macrophages, and osteoclasts as well as their use as phagocytic immune cells in immunology and infection biology. CD14 belongs to the family of LPS receptor antigens and is strongly expressed on the majority of Monocytes.
Human Cryopreserved CD19 B-Cells (pdf) B cells are lymphocytes that play a large role in the humoral immune response. The principal functions of B cells are to make antibodies against antigens, perform the role of antigen-presenting cells (APCs) and eventually develop into memory B cells after activation by antigen interaction. B cells are an essential component of the adaptive immune system.
Human Cryopreserved CD56 Natural Killer Cells (pdf) Natural Killer cells are large granular lymphocytes that are a type of cytotoxic lymphocyte critical to the innate immune system. They are differentiated from the common lymphoid progenitor and are known to differentiate and mature in bone marrow, lymph node, tonsils, spleen, and the thymus where the y then enter into circulation. NK cells express the surface marker CD56 in humans and provide rapid responses to virally infected cells. They respond to tumor formation and have the ability to recognize stressed cells in the absence of antibodies and major histocompatibility complex, allowing for a much faster immune reaction. Applications include analysis of receptor expression, cytokine secretion, or cell-cell interaction with other cells of the immune system.
Human Peripheral Blood Mononuclear Cells (PBMCs) (pdf) A peripheral blood mononuclear cell (PBMC) is defined as any blood cell with a round nucleus (i.e. a lymphocyte, a monocyte, or a macrophage). These blood cells are a critical component in the immune system to fight infection and adapt to intruders. The lymphocyte population consists of CD4+ and CD8+ T cells, B cells and Natural Killer cells, CD14+ Monocytes, and Basophils/Neutrophils/Eosinophils/Dendritic cells. These cells are often extracted from whole blood or from Leukopak using Ficoll, a hydrophilic polysaccharide that separates layers of blood, with monocytes and lymphocytes forming a buffy coat under a layer of plasma. This buffy coat contains the PBMCs.
Human Umbilical Cord Blood CD34+ Progenitor Cell Manual (pdf) CD34 is a well-known marker for primitive and bone marrow-derived progenitor cells, especially for hematopoietic and endothelial progenitors. ZenBio offers CD34+ progenitor cells in a phenotypically undifferentiat ed state. The progenitor cells ar e derived from cord blood of healthy donors and produced at the Zen-Bio facility. Progenitor cells contain two main cellular subpopulations, hematopoietic and endothelial pr ogenitor cells. CD34+ progenitor cells are suitable for a series of studies for directed di fferentiation into more committed types of blood cells and endothelial lineages. Immediately after isolation, the freshly prepared CD34+ progenitor cells are cryopreserved using a serum-free freezing medium. Each vial contains more than 100,000 cells per vial.
Human Primary Hepatocyte Thawing and Culture Manual (pdf)
PRIMARY HEPATOCYTES CULTURE PROTOCOL PROCEDURE The aim of the present method is to describe the process of culturing human cryopreserved hepatocytes. Please read through this entire protocol before attempting this procedure.
Human Hepatic Sinusoidal Endothelial Cell Manual (pdf) Human hepatic sinusoidal endothelial cells (HHSECs) are isolated from human liver obtained via the gift of organ donation from donor tissue that is not suitable for organ transplantation. They are isolated through collagenase digestion and selective cell culture. These cells are a valuable tool for the study of liver physiology and pathophysiology. They are specialized endothelial cells that participate in receptor-mediated clearance of endotoxin, bacteria, and other compounds. They also regulate inflammation, leukocyte recruitment, and host immune responses to pathogens. Frozen HHSECs are cryopreserved at the end of the primary culture
Human Intrahepatic Biliary Epithelial Cell Manual (pdf) Human Intrahepatic Biliary epithelial cells (IHBEC) are isolated from human liver obtained via the gift of organ donation from donor tissue that is not suitable for organ transplantation. IHBECs are the epithelial cells that line the intrahepatic bile ducts. These cells are important in modification of the ductal bile and are targeted in multiple liver diseases, such as primary biliary cirrhosis, cholangiocarcinoma and sclerosing cholangitis. Human Intrahepatic Biliary Epithelial Cells are cryopreserved at the end of the primary culture.
Human Stellate Cells (HP-F-S and P-F-S) (pdf) Hepatic stellate cells (HSCs; also called vitamin A-storing cells, lipocytes, interstitial cells, fat-storing cell, Ito cells) exist in the space between parenchymal cells and sinusoidal endothelial cells of the hepatic lobule. Stellate cells store 80% of vitamin A in the whole body as retinyl palmitate in lipid droplets in the cytoplasm and are responsible for Vitamin A homeostasis. Stellate cells are present in extra hepatic organs such as the pancreas, lung, kidney, intestine, spleen, adrenal gland, ductus deferens and vocal cords. Stellate cells of liver and pancreas and have been described in all vertebrates ranging from lampreys (primitive fish) to humans, demonstrating their major importance.
Human Mammary Basal Epithelial Cells (MBE-F) (pdf) ZenBio's human mammary basal epithelial cells are isolated from mammary tissue of healthy non-diabetic donors between 18 and 60 years old who have undergone elective surgery. The cells are isolated by digestion of the tissue to generate organoid structures collected by centrifugal force. Organoids are maintained in explant culture to stimulate basal myoepithelial cell mi gration and the cells are further purified using immunomagenetic separation. This instruction manual describes procedures to passage and culture the human mammary basal epithelial cells.
Human Mammary Fibroblasts (pdf) Adult mammary fibroblasts are isolated from the breast of healthy donors between 18 and 60 years old undergoing elective breast reduction or mastectomy surgery. The cells are isolated by centrifugal force following enzymatic treatment. This instruction manual describes procedures to passage and culture the human mammary fibroblast cells.
Human Mammary Luminal Epithelial Cell Care Manual (pdf) ZenBio's human mammary luminal epithelial cells are isolated from mammary tissue of healthy non - diabetic donors between 18 and 60 years old who have undergone elective surgery. The cells are isolated by digestion of the tissue to generate organoid structures collected by centrifugal force. Organoids are maintained in explant culture to stimulate Luminal myoepithelial cell migration and the cells are further purified using immunomagenetic separation. This instruction manual describes procedures to passage and culture the human mammary Luminalepithelial cells.
Human Skeletal Muscle Myoblast Manual (pdf) Cultured human skeletal muscle myoblasts. Skeletal muscle is an important site of insulin - stimulated glucose disposal and often the site of insulin resistance in obesity. Human primary cultured skeletal muscle cells can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. ZenBio offers human primary skeletal muscle cells from a variety of donors, including obese donors with Type 2 diabetes.
Human Cryopreserved Melanocytes (pdf) Melanocytes are dendritic cells that are derived from the neural crest cell population in the developing embryo. They are located in the basal layer of the epidermis where they connect with their numerous processes to the surrounding keratinocytes. They represent between 5% and 10% of the total epidermis. Melanocytes synthesize a specific pigment, Melanin in organelles called melanosomes and transfer it to surrounding keratinocytes. It is Melanin that determines skin, eye and hair color.
Human Dermal Fibroblast Manual (pdf) Adult dermal fibroblasts are isolated from the dermis of healthy non-diabetic donors between 18 and 60 years old und ergoing elective surgery. Neonatal dermal fibroblasts are isolated from the foreskins of healthy male newborns. The cells are isolated by centrifugal force following enzymatic treatment or from an explant culture. This instruction manual describes procedures to passage and culture the human dermal fibroblast cells.
Human Keratinocyte Manual (pdf) ZenBio's adult human keratinocytes are isolated from the epidermis of healthy non-diabetic donors between 18 and 60 years old who have undergone elective surgery. ZenBio neonatal keratinocytes are isolated from the foreskin of healthy males aged newborn to infant from elective circumcisions. The cells are isolated by trypsin digestion of the epidermal sheet and collected by centrifugal force. This instruction manual describes procedures to passage and culture the adult and neonatal human keratinocytes. For the adult keratin ocytes, donor matched dermal fibroblasts and preadipocytes are also available for many samples.
Human Adult Stem Cells Manual (pdf) The adipose derived adult stem cells are isolated from subcutaneous adipose tissue of healthy non-diabetic donors between 18 and 60 years old undergoing elective surgery. The cells are isolated by centrifugal force after collagenase treatment. Adult stem cells can be differentiated into various lineages using ZenBio media formulations and protocols. This instruction manual describes procedures to induce human adipose derived adult stem cells (ASC) to differentiate into 1) mature adipocytes, 2) osteo blasts, and 3) chondrocytes. The process of differentiating human adipose-tissue derived adult stem cells to adipocytes has been patent protected by ZenBio under US patent number 6153432.
Human Pericytes (pdf) Pericytes are multipotent mesenchymal-like cells found in association with small blood vessel walls. They are important for angiogenesis, the structural integrity of the microvasculature, and blood flow regulation. However, they can also develop into malignant tumors.
Human Airway Epithelial Cells (pdf)
Human Airway Epithelium contains Epithelial Cells from the upper bronchial/tracheal area and optimized media for their growth.
Human Mesothelial Cells (MES-F) (pdf) Mesothelial cells lining the serous cavities (peritoneal, pericardial, and pleural) and internal organs provide a frictionless barrier and facilitate the movement of opposing organs and tissues. Recently, however, these cells have been found to be pivotal in tumor metastasis, peritoneal dialysis, inflammatory response, and metabolic disease.
Human Tenocyte Care Manual (pdf) ZenBio's human tenocytes are isolated from tendon tissue of healthy donors between 21 and 85 years old who have undergone elective surgery. The cells a re isolated by digestion of the tissue and collected by centrifugal force. This instruction manual describes procedures to passage and culture the human tenocytes.
Human Umbilical Vein Endothelial Cells (HUVECs) (pdf) HUVEC are isolated from the vein of the umbilical cord and are commonly used for physiological and pharmacological investigations, such as macromolecule transport, blood coagulation, angiogenesis, and fibrinolysis. Human umbilical vein endothelial cells (HUVECs) have played a major role as a model system for the study of the regulation of endothelial cell function and the role of the endothelium in the response of the blood vessel wall to stretch, shear forces, and the development of atherosclerotic plaques.
Renal Proximal Convoluted Tubule Epithelial Cells (pdf) Renal proximal tubule cells play an important role in renal function. They reabsorb nearly all of the glucose and amino acids in the glomerular filtrate, while allowing ot her substances of no nutritional value to be excreted. They are also a major site of injury in a variety of congenital, metabolic, and inflammatory diseases with Polycystic kidney disease being one of the more important diseases. Proximal tubule cells can produce inflammatory mediators such as cytokines and chemokines and actively participate in acute inflammatory processes by affecting and directing leukocyte chemotaxis via the production of IL-8. Proximal tubule cells express IL-2R alpha and MHC class II antigens during inflammation after renal transplantation or in cresentic glomerulonephritis indicating the capacity to participate in pathogenesis of immune renal injury.
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