The level of non-esterified fatty acids (NEFA) and glycerol in serum or plasma is indicative of endogenous or induced adipocyte lipolysis. Fatty acids and glycerol are released by adipocytes in response to lipolytic hormones and enter the bloodstream for utilization by other tissues. However, an excess of circulating fatty acid over energy needs may contribute to hyperlipidemia, which is a risk factor for cardiovascular disease.
ZenBio offers kits to analyze the levels of NEFA, glycerol, or both in human and animal serum or plasma. The kits are designed for use in a 96-well format. Through a two-step addition process, the level of NEFA can be quantified relative to a standard curve. Glycerol detection is monitored through a one-step addition and colorimetric assessment.
|SFA-1||Serum/Plasma Fatty Acid Detection Kit - 1 plate||KIT||$356.00|
|SFA-5||Serum/ Plasma Fatty Acid Detection Kit - 5 plate||KIT||$835.00|
|SFA-10||Serum/ Plasma Fatty Acid Detection Kit - 10 plate||KIT||$1,575.00|
|GFA-1||Serum/ Plasma Glycerol and Fatty Acid Detection Kit||KIT||$648.00|
|SGA-1||Serum/ Plasma Glycerol Detection Kit||KIT||$356.00|
|FFA-STAN||Free Fatty Acid Standard,100ul||EA||$36.00|
To see kit manuals
Zen Kits – references
Serum Fatty Acid Plasma Detection Kits
- Free fatty acid kit used to measure levels of non-esterified free fatty acids in fasting mouse serum (Westcott, et al., 2009).
- Non-esterified fatty acids kit used to measure NEFA released into media of mature adipocytes after methionine restriction (Perrone, et al., 2008).
Oleic acid in the absence of a PPARÎ³ agonist increases adipogenic gene expression in bovine muscle satellite cellsX Z Li, Y Yan, J F Zhang, J F Sun, B Sun, C G Yan, S H Choi, B J Johnson, J K Kim,S B Smith
Journal of Animal Science, skz269, https://doi.org/10.1093/jas/skz269
Dietary Glycotoxins Impair Hepatic Lipidemic Profile in Diet-Induced Obese Rats Causing Hepatic Oxidative Stress and Insulin ResistanceC. Neves, T. Rodrigues, J. Sereno, C. Simões, J. Castelhano, J. Gonçalves, G. Bento, S. Gonçalves, R. Seiça, M. R. Domingues, M. Castelo-Branco, and P. Matafome
Endocrinology, en.2019-00236, https://doi.org/10.1210/en.2019-00236
In vivo study of the potential of the carbohydrate-mineral complex from pine nut shells as an ingredient of functional food productsOlga Babich, Lyubov Dyshlyuk, Svetlana Noskova, Stanislav Sukhikh, Alexander Prosekov, Svetlana Ivanova, Valery Pavsky https://doi.org/10.1016/j.bcdf.2019.100185
Lipidomic and transcriptomic analysis of western diet-induced nonalcoholic steatohepatitis (NASH) in female Ldlr-/-miceManuel Garcia-Jaramillo, Melinda H. Spooner, Christiane V. Löhr, Carmen P. Wong, Weijian Zhang, Donald B. Jump
Published: April 3, 2019 https://doi.org/10.1371/journal.pone.0214387
Prepartum fatty acid supplementation in sheep. III. Effect of eicosapentaenoic acid and docosahexaenoic acid during finishing on performance, hypothalamus gene expression, and muscle fatty acids composition in lambs 1Ana Cristina Carranza Martin, Danielle Nicole Coleman, Lyda Guadalupe Garcia, Cecilia C. Furnus, and Alejandro E. Relling
Journal of Animal Science; Champaign Vol. 96, Iss. 12, (Dec 2018): 5300-5310.
Defective fasting-induced PKA activation impairs adipose tissue glycogen degradation in obese Zucker ratsMarcelo Flores-Opazo, Jennifer Trieu, Timur Naim, Denisse Valladares-Ide, Hermann Zbinden-Foncea & David Stapleton
I1-imidazoline receptor-mediated cardiovascular and metabolic effects in high-fat diet-induced metabolic syndrome in ratsAlessandro R. Nascimento, Marcus V. Machado, Cassiano Goncalves-de-Albuquerque, Pascal Bousquet, Eduardo Tibiricá
Prepartum fatty acid supplementation in sheep. III. Effect of eicosapentaenoic acid and docosahexaenoic acid during finishing on performance, hypothalamus gene expression, and muscle fatty acids composition in lambsAna Cristina Carranza Martin Danielle Nicole Coleman Lyda Guadalupe Garcia Cecilia C Furnus Alejandro E Relling
Journal of Animal Science, sky360, https://doi.org/10.1093/jas/sky360
Aldosterone deficiency in mice burdens respiration and accentuates diet-induced hyperinsulinemia and obesityWan-Hui Liao, Claudia Suendermann, Andrea Eva Steuer, Gustavo Pacheco Lopez, Alex Odermatt, Nourdine Faresse, Maciej Henneberg, and Wolfgang Langhans
JCI Insight. 2018;3(14):e99015. https://doi.org/10.1172/jci.insight.99015.
Influence of maternal consumption of different types of fatty acids during pregnancy and lactation on lipid and glucose metabolism of the 21-day-old male offspring in ratsLaís Vales Mennitti, Lila Missae Oyama, Aline Boveto Santamarina, Claudia Maria da Penha Oller do Nascimento, Luciana Pellegrini Pisani
Blockade of CB1 cannabinoid receptor alters gut microbiota and attenuates inflammation and diet-induced obesityPegah Mehrpouya-Bahrami, Kumaraswamy Naidu Chitrala, Mitra S. Ganewatta, Chuanbing Tang, E. Angela Murphy, Reilly T. Enos, Kandy T. Velazquez, Jamie McCellan, Mitzi Nagarkatti & Prakash Nagarkatti
In vivo study of medical and biological properties of functional bakery products with the addition of pumpkin flourLyubov Dyshlyuka, Olga Babichb, Alexander Prosekovb, Svetlana Ivanovaa, Valery Pavskya, Yong Yangc
Caulerpa okamurae extract inhibits adipogenesis in 3T3-L1 adipocytes and prevents high fat diet-induced obesity in C57BL/6 miceBhesh Raj Sharmaa, Hyun Jung Kimb, Min Suk Kima, Chul Min Parka, Dong Young Rhyua
Caulerpa okamurae extract inhibits adipogenesis in 3 T3-L1 adipocytes and prevents high fat diet-induced obesity in C57BL/6 miceBhesh Raj Sharmaa, Hyun Jung Kimb, Min Suk Kima, Chul Min Parka, Dong Young Rhyua
Ratio of dietary rumen degradable protein to rumen undegradable protein affects nitrogen partitioning but does not affect the bovine milk proteome produced by mid- ...R. Tacoma, J. Fields, D.B. Ebenstein, Y.-W. Lam, S.L. Greenwood
Metabolic Abnormalities and Adipose Tissue Leukocyte Dynamics in a Murine Model of Obesity, Weight Loss, and Weight RegainBrian Francis Zamarron
ORCID ID: 0000-0001-6549-4230
The type 2 diabetes susceptibility TCF7L2 gene variants affect postprandial glucose and fat utilization in non-diabetic subjectsE. Adamska, A. Kretowski, J. Goscik, A. Citko, W. Bauer, M. Waszczeniuk, K. Maliszewska, M. Paczkowska-Abdulsalam, M. Niemira, L. Szczerbinski, M. Ciborowski, M. Gorska
Involvement of a gut-retina axis in protection against dietary glycemia-induced age-related macular degenerationSheldon Rowana, Shuhong Jianga, Tal Koremb, Jedrzej Szymanskid, Min-Lee Changa, Jason Szeloga, Christa Cassalmane, Kalavathi Dasuria, Christina McGuiref, Ryoji Nagaig, Xue-Liang Duh, Michael Brownleeh, Naila Rabbanii, Paul J. Thornalleyi, James D. Balejaf, Amy A. Deikj, Kerry A. Piercej, Justin M. Scottj, Clary B. Clishj, Donald E. Smitha, Adina Weinbergerb,c, Tali Avnit-Sagib,c, Maya Lotan-Pompanb,c, Eran Segalb,c, and Allen Taylor
Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissueSharma Bhavya, Pei San Lew, Tooru M. Mizuno
Diabetes 2016 Nov; db160500. http://dx.doi.org/10.2337/db16-0500
Nitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant ratsFrank T. Spradley, Jennifer M Sasser, Jacqueline B Musall, Jennifer C. Sullivan, Joey P. Granger
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology Published 17 August 2016 Vol. no. , DOI: 10.1152/ajpregu.00285.2016
ABCC1 confers tissue-specific sensitivity to cortisol versus corticosterone: A rationale for safer glucocorticoid replacement therapyMark Nixon, Scott D. Mackenzie, Ashley I. Taylor, Natalie Z. M. Homer, Dawn E. Livingstone, Rabah Mouras, Ruth A. Morgan, Damian J. Mole, Roland H. Stimson, Rebecca M. Reynolds, Alistair P. D. Elfick, Ruth Andrew and Brian R. Walker
Science Translational Medicine 17 Aug 2016:Vol. 8, Issue 352, pp. 352ra109, DOI: 10.1126/scitranslmed.aaf9074
The Effects of Supplementation with p-Synephrine Alone and in Combination with Caffeine on Metabolic, Lipolytic, and Cardiovascular Responses during Resistance ...Nicholas A. Ratamess PhD, Jill A. Bush PhD, Jie Kang PhD, William J. Kraemer PhD FACN, Sidney J. Stohs PhD CNS FACN, Vincenzo G. Nocera BS, Megan D. Leise BS, Keith B. Diamond BS, Sara C. Campbell PhD, Howard B. Miller BS and Avery D. Faigenbaum EdD
Protective effects of phyllanthin, a lignan from Phyllanthus amarus, against progression of high fat diet induced metabolic disturbances in miceSneha Jagtap, Pragyanshu Khare, Priyanka Mangal, Mahendra Bishnoi, Kanthi Kiran Kondepudi and Kamlesh Kumar Bhutani
RSC Adv., 2016, DOI: 10.1039/C6RA10774E
Central sympathetic modulation reverses microvascular alterations in a rat model of high-fat diet-induced metabolic syndromeAlessandro R. Nascimento, Marcus V. Machado, Fabiana Gomes, Aline B. Vieira, Cassiano Gonçalves-de-Albuquerque, Marcos A. Lessa, Pascal Bousquet and Eduardo Tibiriçá
Intake of Meals Containing High Levels of Carbohydrates or High Levels of Unsaturated Fatty Acids Induces Postprandial Dysmetabolism in Young Overweight/Obese MenEdyta Adamska, Lucyna Ostrowska, Joanna Gościk, Magdalena Waszczeniuk, Adam Krętowski, and Maria Górska
BioMed Research International
Volume 2015 (2015), Article ID 147196, 9 pages
Characterization of the bovine milk proteome in early-lactation Holstein and jersey breeds of dairy cowsRinske Tacomaa, Julia Fieldsb, David B. Ebensteina, Ying-Wai Lamb, Sabrina L. Greenwooda
Interleukin-15 Receptor alpha (IL15Ra) is a determinant of muscle fuel utilization and its loss protects against obesity.Emanuele Loro, Erin L. Seifert, Cynthia Moffat, Freddy Romero, Manoj Mishra, Zheng Sun, Predrag Krajacic, Frederick Anokye-Danso, Ross S Summer, Rexford S Ahima, Tejvir S. Khurana
American Journal of Physiology - Regulatory, Integrative and Comparative Physiology Published 12 August 2015 Vol. no., DOI: 10.1152/ajpregu.00505.2014
Hepatic genome-wide expression of lipid metabolism in diet-induced obesity rats treated with cocoa polyphenolsFaisal Ali, Amin Ismail, Norhaizan Mohd Esa, Chong Pei Pei, Sander Kersten
Journal of Functional Foods, Volume 17, August 2015
Published: June 12, 2015DOI: 10.1371/journal.pgen.1005287
Protective effects of polyphenol-rich blackcurrant preparation on biochemical and metabolic biomarkers of rats fed a diet high in fructoseJ. Jaroslawska, M. Wroblewska, J. Juskiewicz, L. Brzuzan and Z. Zdunczyk
Article first published online: 3 MAY 2015
Lipid-Encapsulated Echium Oil (Echium plantagineum) Increases the Content of Stearidonic Acid in Plasma Lipid Fractions and Milk Fat of Dairy CattleMelissa Bainbridge, Adam Leslie Lock, and Jana Kraft
J. Agric. Food Chem., DOI: 10.1021/acs.jafc.5b00857
Acute Treatment with XMetA Activates Hepatic Insulin Receptors and Lowers Blood Glucose in Normal MiceDaniel H. Bedinger, Dorothy A. Kieffer, Ira D. Goldfine, Marina K. Roell and Sean H. Adams
DOI: 10.1002/jcb.25168 (Journal of Cellular Biochemistry)
Methionine restriction effects on 11-HSD1 activity and lipogenic/lipolytic balance in F344 rat adipose tissue.Perrone, Carmen E, Mattocks, Dwight A L, Hristopoulos, George, Plummer, Jason D, Krajcik, Rozlyn A, & Orentreich, Norman 2008.
Journal of lipid research; 49: 12-23.
The rs340874 PROX1 type 2 diabetes mellitus risk variant is associated with visceral fat accumulation and alterations in postprandial glucose and lipid metabolismAdam Kretowski1, 2, Edyta Adamska2, Katarzyna Maliszewska1, Natalia Wawrusiewicz-Kurylonek1, Anna Citko1, 2, Joanna Goscik3, Witold Bauer1, 2, Juliusz Wilk1, Anna Golonko4, Magdalena Waszczeniuk1, 4, Danuta Lipinska1, Justyna Hryniewicka1, Magdalena Niemira2, Magdalena Paczkowska2, Michal Ciborowski2 and Maria Gorska1...
- Genes & nutrition, 2015.
Livers with Constitutive mTORC1 Activity Resist Steatosis Independent of Feedback Suppression of Akt.Heidi L. Kenerson, Savitha Subramanian, Rebecca McIntyre, Machiko Kazami, Raymond S. Yeung
Published: February 03, 2015DOI: 10.1371/journal.pone.0117000
Maintenance of skeletal muscle energy homeostasis during prolonged wintertime fasting in the raccoon dog (Nyctereutes procyonoides)Sanni Kinnunen, Satu Mänttäri, Karl-Heinz Herzig, Petteri Nieminen, Anne-Mari Mustonen, Seppo Saarela
Journal of Comparative Physiology B