Serum / Plasma Fatty Acid and Glycerol Detection Kits
The level of non-esterified fatty acids (NEFA) and glycerol in serum or plasma is indicative of endogenous or induced adipocyte lipolysis. Fatty acids and glycerol are released by adipocytes in response to lipolytic hormones and enter the bloodstream for utilization by other tissues. However, an excess of circulating fatty acid over energy needs may contribute to hyperlipidemia, which is a risk factor for cardiovascular disease.
ZenBio offers kits to analyze the levels of NEFA, glycerol, or both in human and animal serum or plasma. The kits are designed for use in a 96-well format. Through a two-step addition process, the level of NEFA can be quantified relative to a standard curve. Glycerol detection is monitored through a one-step addition and colorimetric assessment.
Ordering Information
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| SFA-1 | Serum/Plasma Fatty Acid Detection Kit - 1 plate | KIT | $460.00 |
| SFA-5 | Serum/ Plasma Fatty Acid Detection Kit - 5 plate | KIT | $1078.00 |
| SFA-10 | Serum/ Plasma Fatty Acid Detection Kit - 10 plate | KIT | $2033.00 |
| GFA-1 | Serum/ Plasma Glycerol and Fatty Acid Detection Kit | KIT | $837.00 |
| SGA-1 | Serum/ Plasma Glycerol Detection Kit | KIT | $460.00 |
| FFA-STAN | Free Fatty Acid Standard,100ul | EA | $46.00 |
To see kit manuals
Serum / Plasma Fatty Acid and Glycerol Detection Kits Publications
Hypoglycaemic and Antioxidant Properties of Acrocomia aculeata (Jacq.) Lodd Ex Mart. Extract Are Associated with Better Vascular Function of Type 2 Diabetic Rats
Tamaeh Monteiro-Alfredo, Sara Oliveira, Andreia Amaro, Daniela Rosendo-Silva, Katia Antunes, Ana Salomé Pires, Ricardo Teixo, Ana Margarida Abrantes, Maria Filomena Botelho, Miguel Castelo-Branco, Raquel Seiça, Sónia Silva, Kely de Picoli Souza, and Paulo MatafomeNutrients 2021, 13(8), 2856; https://doi.org/10.3390/nu13082856
Elevated S-adenosylhomocysteine induces adipocyte dysfunction to promote alcohol-associated liver steatosis
Madan Kumar Arumugam, Srinivas Chava, Karuna Rasineni, Matthew C. Paal, Terrence M. Donohue Jr., Natalia A. Osna & Kusum K. KharbandaDOI: https://doi.org/10.1038/s41598-021-94180-x
Separating Insulin-Mediated and Non-Insulin-Mediated Glucose Uptake during Aerobic Exercise in People with Type 1 Diabetes
Thanh-Tin P. Nguyen, Peter G. Jacobs, Jessica Castle, Leah M. Wilson, Kerry Kuehl, Deborah Branigan, Virginia Gabo, Florian Guillot, Michael C. Riddell, Ahmad Haidar, and Joseph El Youssef28 DEC 2020https://doi.org/10.1152/ajpendo.00534.2020
Sea Buckthorn and Rosehip Oils with Chokeberry Extract to Prevent Hypercholesterolemia in Mice Caused by a High-Fat Diet In Vivo
Lubov Tereshchuk, Kseniya Starovoytova, Olga Babich, Lyubov Dyshlyuk, Irina Sergeeva, Valery Pavsky, Svetlana Ivanova, and Alexander ProsekovNutrients 2020, 12, 2941; doi:10.3390/nu12102941
GLP-1 improves adipose tissue glyoxalase activity and capillarization improving insulin sensitivity in type 2 diabetes
Tiago Rodrigues, PatrÃcia Borges, Laura Mar, Daniela Marques, Miguel Albano, Hans Eickhoff, Catarina Carrêlo, Bruno Almeida, Salomé Pires, Margarida Abrantes, Beatriz Martins, Cristina Uriarte, Filomena Botelho, Pedro Gomes, Sónia Silvac, Raquel Seiça, Paulo Matafomehttps://doi.org/10.1016/j.phrs.2020.105198
Laquinimod Prevents Adipogenesis and Obesity by Down-Regulating PPAR-γ and C/EBPα through Activating AMPK
Guang Wang, Bing Wu, Lening Zhang, Yang Cui, Bo Zhang, and Heyuan Wanghttps://doi.org/10.1021/acsomega.0c02525
Impact of cigarette smoke and aerobic physical training on histological and molecular markers of prostate health in rats
A.S.C. Veras, D.B. Baptista, N.J. dos Santos, H.H.A. Thorpe, P.M. Seraphim, A.R. Florido Neto, G.R. Teixeirahttps://doi.org/10.1590/1414-431x20209108
Long-term hypercortisolism induces lipogenesis promoting palmitic acid accumulation and inflammation in visceral adipose tissue compared to HFD-induced obesity
Guillermo García-Eguren, Aleix Sala-Vila, Oriol Giró, Arturo Vega-Beyhart, and Felicia A. Hanzu21 APR 2020 https://doi.org/10.1152/ajpendo.00516.2019
Ghrelin antagonist regulates metabolic hormone receptorsin the hypothalamus of ewes
Ana C. Carranza-Martin, Anthony. J. Parker, Cecilia. C. Furnus, Alejandro. E. Rellinghttps://doi.org/10.1016/j.smallrumres.2020.106091
Associations of appetite sensations and metabolic characteristics with weight retention in postpartum women
Leticia C.R. Pereira, Sarah A. Elliott, Linda J. McCargar, Rhonda C Bell, Paula J Robson, Carla M. Prado, PhDApplied Physiology, Nutrition, and Metabolism, https://doi.org/10.1139/apnm-2019-0809
The ergogenic activity of Cider vinegar: A randomized cross-over, double-blind, clinical trial
Hui-Fang Chiu, Michael Chiang, Hui-Ju Liao, You-Cheng Shen, Kamesh Venkatakrishnan, I-Shiung Cheng, Chin-Kun Wanghttps://doi.org/10.1016/j.smhs.2020.02.001
Role of Hepatic Glucocorticoid Receptor in Metabolism in Models of 5aR1 Deficiency in Male Mice
Tracy C. S. Mak, Dawn E. W. Livingstone, Mark Nixon, Brian R. Walker, and Ruth AndrewEndocrinology, Volume 160, Issue 9, September 2019, Pages 2061–2073, https://doi.org/10.1210/en.2019-00236
Examining the effects of a high-protein total diet replacement on energy metabolism, metabolic blood markers, and appetite sensations in healthy adults: protocol for two complementary, randomized, controlled, crossover trials
Camila L. P. Oliveira, Normand G. Boulé, Arya M. Sharma, Sarah Elliott, Mario Siervo, Sunita Ghosh, Aloys Berg & Carla M. PradoDOI: https://doi.org/10.1186/s13063-019-3950-y
Stroke Induces Prolonged Changes in Lipid Metabolism, the Liver and Body Composition in Mice
Michael J. Haley, Claire S. White, Daisy Roberts, Kelly O'Toole, Catriona J. Cunningham, Jack Rivers-Auty, Conor O'Boyle, Conor Lane, Oliver Heaney, Stuart M. Allan & Catherine B. LawrenceDOI: https://doi.org/10.1007/s12975-019-00763-2
Therapeutic lifestyle change intervention improved metabolic syndrome criteria and is complementary to amlodipine/atorvastatin
Hanaa S. Sallam, Demidmaa R. Tuvdendorj, Ishwarlal Jialal, Manisha Chandalia, Nicola Abatehttps://doi.org/10.1016/j.jdiacomp.2019.107480
Circadian clock network desynchrony promotes weight gain and alters glucose homeostasis in mice
Isa Kolbe, Brinja Leinweber, Matthias Brandenburger, Henrik Oster
https://doi.org/10.1016/j.molmet.2019.09.012
Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells
X Z Li, Y Yan, J F Zhang, J F Sun, B Sun, C G Yan, S H Choi, B J Johnson, J K Kim, S B SmithJournal of Animal Science, skz269, https://doi.org/10.1093/jas/skz269
Dietary Glycotoxins Impair Hepatic Lipidemic Profile in Diet-Induced Obese Rats Causing Hepatic Oxidative Stress and Insulin Resistance
C. Neves, T. Rodrigues, J. Sereno, C. Simões, J. Castelhano, J. Gonçalves, G. Bento, S. Gonçalves, R. Seiça, M. R. Domingues, M. Castelo-Branco, and P. Matafomehttps://doi.org/10.1155/2019/6362910
Role of hepatic glucocorticoid receptor in metabolism in models of 5αR1 Deficiency in Male Mice
Tracy CS Mak, Dawn EW Livingstone, Mark Nixon, Brian R Walker, Ruth AndrewEndocrinology, en.2019-00236, https://doi.org/10.1210/en.2019-00236
In vivo study of the potential of the carbohydrate-mineral complex from pine nut shells as an ingredient of functional food products
Olga Babich, Lyubov Dyshlyuk, Svetlana Noskova, Stanislav Sukhikh, Alexander Prosekov, Svetlana Ivanova, Valery Pavsky https://doi.org/10.1016/j.bcdf.2019.100185Lipidomic and transcriptomic analysis of western diet-induced nonalcoholic steatohepatitis (NASH) in female Ldlr-/-mice
Manuel Garcia-Jaramillo, Melinda H. Spooner, Christiane V. Löhr, Carmen P. Wong, Weijian Zhang, Donald B. JumpPublished: April 3, 2019 https://doi.org/10.1371/journal.pone.0214387
Prepartum fatty acid supplementation in sheep. III. Effect of eicosapentaenoic acid and docosahexaenoic acid during finishing on performance, hypothalamus gene expression, and muscle fatty acids composition in lambs 1
Ana Cristina Carranza Martin, Danielle Nicole Coleman, Lyda Guadalupe Garcia, Cecilia C. Furnus, and Alejandro E. RellingJournal of Animal Science; Champaign Vol. 96, Iss. 12, (Dec 2018): 5300-5310.
Defective fasting-induced PKA activation impairs adipose tissue glycogen degradation in obese Zucker rats
Marcelo Flores-Opazo, Jennifer Trieu, Timur Naim, Denisse Valladares-Ide, Hermann Zbinden-Foncea & David Stapletonhttps://doi.org/10.1038/s41366-019-0327-y
I1-imidazoline receptor-mediated cardiovascular and metabolic effects in high-fat diet-induced metabolic syndrome in rats
Alessandro R. Nascimento, Marcus V. Machado, Cassiano Goncalves-de-Albuquerque, Pascal Bousquet, Eduardo TibiricáDOI: https://doi.org/10.1016/j.autneu.2018.12.007
Prepartum fatty acid supplementation in sheep. III. Effect of eicosapentaenoic acid and docosahexaenoic acid during finishing on performance, hypothalamus gene expression, and muscle fatty acids composition in lambs
Ana Cristina Carranza Martin Danielle Nicole Coleman Lyda Guadalupe Garcia Cecilia C Furnus Alejandro E RellingJournal of Animal Science, sky360, https://doi.org/10.1093/jas/sky360
Serum CK 18-M30 reflect liver pathological severity during NAFLD progression in a rat model
Li Xue, Xiaolan Lu, Juntao He, Ting Zhang, Xiaokang Wu, Yanping Zhang, Ningning Wang, Zhe An, Jiru Xu, Yan Genghttps://doi.org/10.1016/j.prp.2018.08.016
Aldosterone deficiency in mice burdens respiration and accentuates diet-induced hyperinsulinemia and obesity
Wan-Hui Liao, Claudia Suendermann, Andrea Eva Steuer, Gustavo Pacheco Lopez, Alex Odermatt, Nourdine Faresse, Maciej Henneberg, and Wolfgang LanghansJCI Insight. 2018;3(14):e99015. https://doi.org/10.1172/jci.insight.99015.
Influence of maternal consumption of different types of fatty acids during pregnancy and lactation on lipid and glucose metabolism of the 21-day-old male offspring in rats
Laís Vales Mennitti, Lila Missae Oyama, Aline Boveto Santamarina, Claudia Maria da Penha Oller do Nascimento, Luciana Pellegrini Pisanihttps://doi.org/10.1016/j.plefa.2018.07.001
The tumor secretory factor ZAG promotes white adipose tissue browning and energy wasting
Sawsan Elattar, Manali Dimri, and Ande Satyanarayanahttps://doi.org/10.1096/fj.201701465RR
Blockade of CB1 cannabinoid receptor alters gut microbiota and attenuates inflammation and diet-induced obesity
Pegah Mehrpouya-Bahrami, Kumaraswamy Naidu Chitrala, Mitra S. Ganewatta, Chuanbing Tang, E. Angela Murphy, Reilly T. Enos, Kandy T. Velazquez, Jamie McCellan, Mitzi Nagarkatti & Prakash Nagarkattidoi:10.1038/s41598-017-15154-6
In vivo study of medical and biological properties of functional bakery products with the addition of pumpkin flour
Lyubov Dyshlyuka, Olga Babichb, Alexander Prosekovb, Svetlana Ivanovaa, Valery Pavskya, Yong Yangchttps://doi.org/10.1016/j.bcdf.2017.09.001
Caulerpa okamurae extract inhibits adipogenesis in 3T3-L1 adipocytes and prevents high fat diet-induced obesity in C57BL/6 mice
Bhesh Raj Sharmaa, Hyun Jung Kimb, Min Suk Kima, Chul Min Parka, Dong Young Rhyuahttps://doi.org/10.1016/j.nutres.2017.09.002
Caulerpa okamurae extract inhibits adipogenesis in 3 T3-L1 adipocytes and prevents high fat diet-induced obesity in C57BL/6 mice
Bhesh Raj Sharmaa, Hyun Jung Kimb, Min Suk Kima, Chul Min Parka, Dong Young Rhyuahttps://doi.org/10.1016/j.nutres.2017.09.002
Ratio of dietary rumen degradable protein to rumen undegradable protein affects nitrogen partitioning but does not affect the bovine milk proteome produced by mid- ...
R. Tacoma, J. Fields, D.B. Ebenstein, Y.-W. Lam, S.L. GreenwoodDOI: http://dx.doi.org/10.3168/jds.2017-12647
Metabolic Abnormalities and Adipose Tissue Leukocyte Dynamics in a Murine Model of Obesity, Weight Loss, and Weight Regain
Brian Francis ZamarronORCID ID: 0000-0001-6549-4230
The type 2 diabetes susceptibility TCF7L2 gene variants affect postprandial glucose and fat utilization in non-diabetic subjects
E. Adamska, A. Kretowski, J. Goscik, A. Citko, W. Bauer, M. Waszczeniuk, K. Maliszewska, M. Paczkowska-Abdulsalam, M. Niemira, L. Szczerbinski, M. Ciborowski, M. GorskaDOI: http://dx.doi.org/10.1016/j.diabet.2017.05.001
Involvement of a gut-retina axis in protection against dietary glycemia-induced age-related macular degeneration
Sheldon Rowana, Shuhong Jianga, Tal Koremb, Jedrzej Szymanskid, Min-Lee Changa, Jason Szeloga, Christa Cassalmane, Kalavathi Dasuria, Christina McGuiref, Ryoji Nagaig, Xue-Liang Duh, Michael Brownleeh, Naila Rabbanii, Paul J. Thornalleyi, James D. Balejaf, Amy A. Deikj, Kerry A. Piercej, Justin M. Scottj, Clary B. Clishj, Donald E. Smitha, Adina Weinbergerb,c, Tali Avnit-Sagib,c, Maya Lotan-Pompanb,c, Eran Segalb,c, and Allen Taylordoi: 10.1073/pnas.1702302114
Central action of xenin affects the expression of lipid metabolism-related genes and proteins in mouse white adipose tissue
Sharma Bhavya, Pei San Lew, Tooru M. MizunoDOI: http://dx.doi.org/10.1016/j.npep.2017.01.007
Metabolic Indicators for Early Pregnancy in Zebu and Crossbred Dairy Cows reared under sub-tropical Climate 2
Muzamil ABDULLAH, Tushar Kumar MOHANTY, Mukesh BHAKAT, Arumugam KUMARESAN, Tapas Kumar PATBANDHA, Ambadas Rakhamaji MADKAR and Ashok Kumar MOHANTYMacrophage Proliferation Sustains Adipose Tissue Inflammation in Formerly Obese Mice
Brian F. Zamarron, Taleen A. Mergian, Kae Won Cho, Gabriel Martinez-Santibanez, Danny Luan, Kanakadurga Singer, Jennifer L. DelProposto, Lynn M. Geletka, Lindsey A. Muir, Carey N. LumengDiabetes 2016 Nov; db160500. http://dx.doi.org/10.2337/db16-0500
Nitric oxide synthase-mediated blood pressure regulation in obese melanocortin-4 receptor-deficient pregnant rats
Frank T. Spradley, Jennifer M Sasser, Jacqueline B Musall, Jennifer C. Sullivan, Joey P. GrangerAmerican Journal of Physiology - Regulatory, Integrative and Comparative Physiology Published 17 August 2016 Vol. no. , DOI: 10.1152/ajpregu.00285.2016
ABCC1 confers tissue-specific sensitivity to cortisol versus corticosterone: A rationale for safer glucocorticoid replacement therapy
Mark Nixon, Scott D. Mackenzie, Ashley I. Taylor, Natalie Z. M. Homer, Dawn E. Livingstone, Rabah Mouras, Ruth A. Morgan, Damian J. Mole, Roland H. Stimson, Rebecca M. Reynolds, Alistair P. D. Elfick, Ruth Andrew and Brian R. WalkerScience Translational Medicine 17 Aug 2016:Vol. 8, Issue 352, pp. 352ra109, DOI: 10.1126/scitranslmed.aaf9074
The Effects of Supplementation with p-Synephrine Alone and in Combination with Caffeine on Metabolic, Lipolytic, and Cardiovascular Responses during Resistance ...
Nicholas A. Ratamess PhD, Jill A. Bush PhD, Jie Kang PhD, William J. Kraemer PhD FACN, Sidney J. Stohs PhD CNS FACN, Vincenzo G. Nocera BS, Megan D. Leise BS, Keith B. Diamond BS, Sara C. Campbell PhD, Howard B. Miller BS and Avery D. Faigenbaum EdDDOI:10.1080/07315724.2016.1150223
Protective effects of phyllanthin, a lignan from Phyllanthus amarus, against progression of high fat diet induced metabolic disturbances in mice
Sneha Jagtap, Pragyanshu Khare, Priyanka Mangal, Mahendra Bishnoi, Kanthi Kiran Kondepudi and Kamlesh Kumar BhutaniRSC Adv., 2016, DOI: 10.1039/C6RA10774E
Central sympathetic modulation reverses microvascular alterations in a rat model of high-fat diet-induced metabolic syndrome
Alessandro R. Nascimento, Marcus V. Machado, Fabiana Gomes, Aline B. Vieira, Cassiano Gonçalves-de-Albuquerque, Marcos A. Lessa, Pascal Bousquet and Eduardo TibiriçáDOI: 10.1111/micc.12280
Intake of Meals Containing High Levels of Carbohydrates or High Levels of Unsaturated Fatty Acids Induces Postprandial Dysmetabolism in Young Overweight/Obese Men
Edyta Adamska, Lucyna Ostrowska, Joanna Gościk, Magdalena Waszczeniuk, Adam Krętowski, and Maria GórskaBioMed Research International
Volume 2015 (2015), Article ID 147196, 9 pages
http://dx.doi.org/10.1155/2015/147196
Characterization of the bovine milk proteome in early-lactation Holstein and jersey breeds of dairy cows
Rinske Tacomaa, Julia Fieldsb, David B. Ebensteina, Ying-Wai Lamb, Sabrina L. Greenwoodadoi:10.1016/j.jprot.2015.09.024
Interleukin-15 Receptor alpha (IL15Ra) is a determinant of muscle fuel utilization and its loss protects against obesity.
Emanuele Loro, Erin L. Seifert, Cynthia Moffat, Freddy Romero, Manoj Mishra, Zheng Sun, Predrag Krajacic, Frederick Anokye-Danso, Ross S Summer, Rexford S Ahima, Tejvir S. KhuranaAmerican Journal of Physiology - Regulatory, Integrative and Comparative Physiology Published 12 August 2015 Vol. no., DOI: 10.1152/ajpregu.00505.2014
Hepatic genome-wide expression of lipid metabolism in diet-induced obesity rats treated with cocoa polyphenols
Faisal Ali, Amin Ismail, Norhaizan Mohd Esa, Chong Pei Pei, Sander KerstenJournal of Functional Foods, Volume 17, August 2015
Hypothalamic Biomarker of Ambient Temperature Independent of Nutritional Status
Julia Jaroslawska, Agnieszka Chabowska-Kita, Monika M. Kaczmarek, Leslie P. KozakPublished: June 12, 2015DOI: 10.1371/journal.pgen.1005287
Protective effects of polyphenol-rich blackcurrant preparation on biochemical and metabolic biomarkers of rats fed a diet high in fructose
J. Jaroslawska, M. Wroblewska, J. Juskiewicz, L. Brzuzan and Z. ZdunczykArticle first published online: 3 MAY 2015
DOI: 10.1111/jpn.12321
Lipid-Encapsulated Echium Oil (Echium plantagineum) Increases the Content of Stearidonic Acid in Plasma Lipid Fractions and Milk Fat of Dairy Cattle
Melissa Bainbridge, Adam Leslie Lock, and Jana KraftJ. Agric. Food Chem., DOI: 10.1021/acs.jafc.5b00857
Acute Treatment with XMetA Activates Hepatic Insulin Receptors and Lowers Blood Glucose in Normal Mice
Daniel H. Bedinger, Dorothy A. Kieffer, Ira D. Goldfine, Marina K. Roell and Sean H. AdamsDOI: 10.1002/jcb.25168 (Journal of Cellular Biochemistry)
The inhibitory effect of genistein on hepatic steatosis is linked to visceral adipocyte metabolism in mice with diet-induced non-alcoholic fatty liver disease.
Mi-Hyun Kim, Kyung-Sun Kang and Yeon-Sook Lee (2010). British Journal of Nutrition, 104, pp 1333-1342 doi:10.1017/S0007114510002266.MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity.
Westcott, D. J., Delproposto, J. B., Geletka, L. M., Wang, T., Singer, K., Saltiel, A. R., et al. 2009. J Exp Med; 206: 3143-3156.Methionine restriction effects on 11-HSD1 activity and lipogenic/lipolytic balance in F344 rat adipose tissue.
Perrone, Carmen E, Mattocks, Dwight A L, Hristopoulos, George, Plummer, Jason D, Krajcik, Rozlyn A, & Orentreich, Norman 2008.Journal of lipid research; 49: 12-23.
The rs340874 PROX1 type 2 diabetes mellitus risk variant is associated with visceral fat accumulation and alterations in postprandial glucose and lipid metabolism
Adam Kretowski1, 2, Edyta Adamska2, Katarzyna Maliszewska1, Natalia Wawrusiewicz-Kurylonek1, Anna Citko1, 2, Joanna Goscik3, Witold Bauer1, 2, Juliusz Wilk1, Anna Golonko4, Magdalena Waszczeniuk1, 4, Danuta Lipinska1, Justyna Hryniewicka1, Magdalena Niemira2, Magdalena Paczkowska2, Michal Ciborowski2 and Maria Gorska1...- Genes & nutrition, 2015.
Livers with Constitutive mTORC1 Activity Resist Steatosis Independent of Feedback Suppression of Akt.
Heidi L. Kenerson, Savitha Subramanian, Rebecca McIntyre, Machiko Kazami, Raymond S. YeungPublished: February 03, 2015DOI: 10.1371/journal.pone.0117000
Maintenance of skeletal muscle energy homeostasis during prolonged wintertime fasting in the raccoon dog (Nyctereutes procyonoides)
Sanni Kinnunen, Satu Mänttäri, Karl-Heinz Herzig, Petteri Nieminen, Anne-Mari Mustonen, Seppo SaarelaJournal of Comparative Physiology B
February 2015
Zen Kits – references
Serum Fatty Acid Plasma Detection Kits
- Free fatty acid kit used to measure levels of non-esterified free fatty acids in fasting mouse serum (Westcott, et al., 2009).
- Non-esterified fatty acids kit used to measure NEFA released into media of mature adipocytes after methionine restriction (Perrone, et al., 2008).
