Skeletal Myoblasts
Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes.
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. Skeletal myoblasts are available from both male and female donors. Pooled lots of cells are available to provide large lots for screening and to decrease issues with patient to patient variability.
Cells are available cryopreserved (500,000 cells/vial).
QUALITY CONTROL: All cells have been tested for myotube differentiation. Cells have tested negative for HIV-1, HIV-2, HTLC-1, HTLV-2, Hepatitis B, Hepatitis C and mycoplasma. Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. Myogenic differentiation was verified by immunohistochemical staining using antibodies to desmin and myosin. The differentiated myocytes also respond to insulin stimulated glucose uptake.
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts.
Ordering Information:
Cryopreserved skeletal myoblasts
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| SKB-F | Cryopreserved Human Skeletal Myoblasts, (0.5 x 106 cells/vial) | Vial | $594.00 |
| SKB-F-SL | Cryopreserved Human Skeletal Myoblasts, (0.5 x 106 cells/vial), POOLED donor lot | Vial | $594.00 |
| SKB-F-1 | Cryopreserved Human Skeletal Myoblasts, LEAN DONOR (BMI <25.0), (0.5 x 106 cells/vial) | Vial | $1307.00 |
| SKM-D-F | Cryopreserved Human Skeletal Myoblasts, DIABETIC Donor, (0.5 x 106 cells/vial) | Vial | $1307.00 |
Skeletal Myoblast Media
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| SKM-D | Skeletal Muscle Cell Differentiation Medium | 500ml | $202.00 |
| SKM-M | Skeletal Muscle Cell Growth Medium | 500ml | $202.00 |
| SKM-50 | Skeletal Myoblast Cryopreservation Medium | 50ml | $132.00 |
| SKM-M-ABF | Skeletal Muscle Cell Growth Medium, WITHOUT Antibiotics | 500ml | $202.00 |
| SKM-100 | Skeletal Muscle Cell Cryopreservation Medium | 100ml | $268.00 |
Contact Us For More Information.
Human Skeletal Muscle Myoblast Manual (pdf)
A comprehensive list of products and prices can be found at Retail Prices(.PDF).
Skeletal Myoblasts Publications
Mitochondrial calcium uptake regulates tumour progression in embryonal rhabdomyosarcoma
Hsin Yao Chiu, Amos Hong Pheng Loh, Reshma Tanejadoi: https://doi.org/10.1101/2021.11.10.468020
N-acetyltaurine and Acetylcarnitine Production for the Mitochondrial Acetyl-CoA Regulation in Skeletal Muscles during Endurance Exercises
Teruo Miyazaki , Yuho Nakamura-Shinya, Kei Ebina, Shoichi Komine, Song-Gyu Ra, Keisuke Ishikura, Hajime Ohmori and Akira HondaMetabolites 2021, 11(8), 522; https://doi.org/10.3390/metabo11080522
In-vivo Transcriptomic Profiling of Systemic Aging using Cell Encapsulation
Omid Mashinchian, Xiaotong Hong, Eugenia Migliavacca, Christophe Boss, Filippo De Franceschi, Joris Michaud, Emmeran Le Moa, Jasmin Collerette-Tremblay, Joan Isern, Sylviane Metairon, Frederic Raymond, Patrick Descombes, Nicolas Bouche, Pura Muññoz-Cánoves, Jerome N. Feige, C. Florian Bentzingerdoi: https://doi.org/10.1101/2020.03.09.979054
Effect of selected drugs on mitochondrial metabolism in an in vitro model of human skeletal muscle
MUDr. Adëla Krajcová, Doctoral ThesisUse of deep neural network ensembles to identify embryonic-fetal transition markers: repression of COX7A1 in embryonic and cancer cells
Michael D. West, Ivan Labat, Hal Sternberg, Dana Larocca, Igor Nasonkin, Karen B. Chapman, Ratnesh Singh, Eugene Makarev, Alex Aliper, Andrey Kazennov, Andrey Alekseenko, Nikolai Shuvalov, Evgenia Cheskidova, Aleksandr Alekseev, Artem Artemov, Evgeny Putin, Polina Mamoshina, Nikita Pryanichnikov, Jacob Larocca, Karen Copeland, Evgeny Izumchenko, Mikhail Korzinkin and Alex ZhavoronkovOncotarget. 2018; 9:7796-7811. https://doi.org/10.18632/oncotarget.23748
Satellite Cell Activity and Self-Renewal in Patients with Cerebral Palsy
Stephanie K YeagerUniversity of Delaware, ProQuest Dissertations Publishing, 2017. 10269621
Association of cultured myotubes and fasting plasma metabolite profiles with mitochondrial dysfunction in Type 2 diabetes subjects
Mohamad Hafizi Abu Bakar and Mohamad Roji SarmidiMol. BioSyst., 2017, Accepted Manuscript , DOI:10.1039/C7MB00333A
Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis
Zhuo-Xian Meng, Jianke Gong, Zhimin Chen, Jingxia Sun, Yuanyuan Xiao, Lin Wang, Yaqiang Li, Jianfeng Liu, X. Z. Shawn Xu, Jiandie D. Lin6,'Correspondence information about the author Jiandie D. LinDOI: http://dx.doi.org/10.1016/j.molcel.2017.04.007
Retention of acetylcarnitine in chronic kidney disease causes insulin resistance in skeletal muscle
Yasunori Miyamoto, Teruo Miyazaki, Akira Honda, Homare Shimohata, Kouichi Hirayama, Masaki Kobayashihttp://doi.org/10.3164/jcbn.15-146
Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis
Chintan K Kikani Xiaoying Wu Litty Paul Hana Sabic Zuolian Shen Arvind Shakya Alexandra Keefe Claudio Villanueva Gabrielle Kardon Barbara Graves Dean Tantin Jared RutterDOI: http://dx.doi.org/10.7554/eLife.17985
Jmjd2C increases MyoD transcriptional activity through inhibiting G9a-dependent MyoD degradation
Eun-Shil Junga, Ye-Ji Sima, Hoe-Su Jeonga, Su-Jin Kima, Ye-Jin Yuna, Joo-Hoon Songb, Su-Hee Jeonc, Chungyoul Choed, Kyung-Tae Parke, Chang-Hoon Kima, , Kye-Seong KimBiochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, July 2015
Celastrol Protects against Antimycin A-Induced Insulin Resistance in Human Skeletal Muscle Cells
Mohamad Hafizi Abu Bakar , Kian-Kai Cheng , Mohamad Roji Sarmidi , Harisun Yaakob and Hasniza Zaman HuriMolecules 2015, 20, 8242-8269; doi:10.3390/molecules20058242
