Cell/Tissue Derived Products

ZenBio offers an extensive array of cell and tissue derived products. Custom collections are available!

Cell Extracts
Total protein extracts have been prepared from cultured subcutaneous and omental preadipocytes and adipocytes. These protein extracts are suitable for Western blotting, ELISA assays or immunoprecipitation. We can prepare custom cell extracts upon request.
Conditioned Media
Conditioned media from cultured subcutaneous and omental preadipocytes and adipocytes are available in 10 ml aliquots. The cells are incubated with serum and phenol red free basal medium for 24 hours. Supernatants are collected and flash frozen. These preparations are suitable for a variety of assays. We can prepare custom conditioned medium from various cell types upon request.
Custom Culture Services
ZenBio offers an extensive array of cell types and plate formats in our regular catalog. When you require a cell type or plating format not currently listed, please let us know. We are able to work with our surgical partners to obtain various additional tissue types as well as isolate various cell types from tissue we currently receive.
Custom Procurement Solutions
ZenBio offers cells, tissue, and serum from a diverse group of donor populations. Products are available from mixed donor lots, individual donor lots and a variety of disease states such as type II diabetes, hypertension, and others. Often, ZenBio is able to provide additional value added information such as blood panels and history of disease. In the event we do not have samples from a donor demographic that meets your needs, we are able to custom tailor a solution.
Flash Frozen Floaters
Primary adipocytes or floaters are isolated from adipose tissue during the initial processing of tissue. These floaters have been flash frozen in liquid nitrogen and can be used for cell extract applications.
Human Skin
ZenBio offers both fresh and flash frozen skin. We have extensive inventory of flash frozen skin from various depots and donor demographics.
Human Tissue / Fluids
ZenBio offers an extensive list of flash frozen human tissues and fluids for your research needs.
Reconstructed Human Epidermis (RHE)
ZenBio, has developed a cost-effective Reconstructed Human Epidermis (RHE) model which mimics the barrier function of human stratum corneum, as well as a complete viable epidermis.
RepiGut Planar-Cell Based Human Gut Model
RepliGut® Planar is a stem cell derived in vitro drug testing system that recapitulates the human intestinal barrier physiology, organized into a polarized monolayer of differentiated epithelial cells with tight junctions.
RNA
High quality RNA is available from an extensive listing of human primary cells and tissues. The RNA is high quality from both normal and diseased donors. Donor information including age, sex, and body mass index will be included with your order. We can accommodate many special requests.

Get the latest Zenbio Publications on:

Total RNA Publications

De novo reconstruction of human adipose transcriptome reveals conserved lncRNAs as regulators of brown adipogenesis

Chunming Ding, Yen Ching Lim, Sook Yoong Chia, Arcinas Camille Esther Walet, Shaohai Xu, Kinyui Alice Lo, Yanling Zhao, Dewen Zhu, Zhihui Shan, Qingfeng Chen, Melvin Khee-Shing Leow, Dan Xu & Lei Sun
Nature Communicationsvolume 9, Article number: 1329 (2018)
doi:10.1038/s41467-018-03754-3

Modulation of the IL-33/IL-13 Axis in Obesity by IL-13Rα2

Jennifer Duffen, Melvin Zhang, Katherine Masek-Hammerman, Angela Nunez, Agnes Brennan, Jessica E. C. Jones, Jeffrey Morin, Karl Nocka and Marion Kasaian
J Immunol January 5, 2018, ji1701256; DOI: https://www.doi.org/10.4049/jimmunol.1701256

Activation of IRF1 in Human Adipocytes Leads to Phenotypes Associated with Metabolic Disease

Max Friesen, Raymond Camahort, Youn-Kyoung Lee, Fang Xia, Robert E. Gerszten, Eugene P. Rhee, Rahul C. Deo, Chad A. Cowan
http://www.doi.org/10.1016/j.stemcr.2017.03.014

MiR-17-92 Cluster Promotes Hepatocarcinogenesis

Hanqing Zhu, Chang Han and Tong Wu
Carcinogenesis (2015)doi: 10.1093/carcin/bgv112

Elevated serum levels of pigment epithelium-derived factor in the metabolic syndrome.

Yamagishi S, Adachi H, Abe A, Yashiro T, Enomoto M, Furuki K, et al. J Clin Endocrinol Metab 2006; 91: 2447-50.

Chemerin-A new adipokine that modulates adipogenesis via its own receptor.

Roh S-G, et al. Biochem Biophys Res Commun. 2007;362:1013-8.

Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual role for FSP27 in adipocyte metabolism and cell death.

Kim, J. Y., K. Liu, S. Zhou, K. Tillison, Y. Wu, and C. M. Smas. 2008. Am. J. Physiol. Endocrinol. Metab. 294:E654-E667.

A futile metabolic cycle activated in adipocytes by antidiabetic agents.

Guan, HP, et al. Nat. Med. 2002. 8:1122-1128.

The synthetic triterpenoid CDDO-Im inhibits fatty acid synthase expression and has antiproliferative and proapoptotic effects in human liposarcoma cells.

Hughes, D. , Martel, P. , Kinlaw, W. and Eisenberg, B. (2008) Cancer Invest 26, pp. 118-127.

Orbital Fibroblast Heterogeneity May Determine the Clinical Presentation of Thyroid-Associated.

Ophthalmopathy Terry J. Smith, Laura Koumas, AnneMarie Gagnon, Andrea Bell, Gregory D. Sempowski, Richard P. Phipps, and Alexander Sorisky J. Clin. Endocrinol. Metab., Jan 2002; 87: 385 - 392.

Reconstructed Human Epidermis (RHE) Publications

Encapsulation and controlled release of retinol from silicone particles for topical delivery

C. Wyatt Shieldsa, John P. White, Erica G. Osta, Jerishma Patela, Shashank Rajkumar, Nickolas Kirby, Jean-Philippe Therrien, Stefan Zauscher
https://doi.org/10.1016/j.jconrel.2018.03.023

Flash Frozen Floaters Publications:

Discovery and characterization of GSK256073, a non-flushing hydroxy-carboxylic acid receptor 2 (HCA2) agonist

Dennis Sprechera, , Miles Maxwellb, Joanne Goodmanb, Brian Whiteb, Chi-Man Tangb, Valerie Boullayc, Anne-Charlotte de Gouville
European Journal of Pharmacology, doi:10.1016/j.ejphar.2015.01.051