ZenBio human pre-adipocytes (adipose derived adult stem cells) and placental mesenchymal stem cells (MSC) are characterized by their self-renewing capacity and ability to differentiate into chondrocytes, adipocytes, and osteocytes. This makes them attractive starting materials for tissue engineering and regenerative medicine applications.
Since few transplanted cells persist in vivo, the beneficial effects of cell therapy may lie in the secreted factors being the active component of this treatment. A key part of paracrine secretion is Exosomes, which are membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane.
- Exosomes contain protein, DNA, and RNA, thus making them an attractive vector of paracrine signals delivered by stem cells.
- Exosomes may also be "loaded" with predetermined proteins and nucleic acid to achieve a desired effect (1).
- Exosomes can be stored as an "off-the-shelf" product having the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
- In vitro, exosomes from pre-adipocytes stimulate cell proliferation in a wound healing model.
- In vivo, adipose-graft derived exosomes have been shown to be a promising tool for skin repair and remodeling (2)
Quality Control: All cells have been screened negative for HIV-1, HIV-2, Hep-B, Hep-C and Syphilis. All isolated and concentrated exosomes undergo includes Particle mean diameter, Protein concentration, RNA concentration and Concentration of particles/ml.
Exosome-mediated fibroblast proliferation
After the cell monolayer was 'wounded' using a cell scraper, the cultures were washed and fed with DMEM + 10% exosome-free FBS (Control), or DMEM + 10% exosome-free FBS supplemented with 25µg of pre-adipocyte exosomes (Exosome Treated). After a 5 day incubation, fibroblast proliferation closed the gap between the two edges of the wounded monolayer only in the medium supplemented with exosomes. These data support the notion that factors including exosomes secreted by pre-adipocytes (adipose tissue-derived MSC) play a role in wound healing and tissue regeneration, by facilitating cellular proliferation
|EXP-F100||Human Preadipocyte (Mesenchymal Stem Cell) Exosomes, Frozen (>1 billion particles/vial)||Vial||$829.00|
|EXPLMSC-F100||Human Placental Derived Mesenchymal Stem Cell Exosomes, Frozen (>1 billion particles/vial)||Vial||$1022.00|
|EXCBS-F100||Human Cord Blood Serum Exosomes, Frozen (>1 billion particles/vial)||Vial||$1086.00|
|CA-09||Custom Human Exosome Isolation by Ultracentrifugation from customer provided conditioned medium (Up to 390mls) Includes full QC.||Each||$643.00|
*Custom Exosome Isolations from Tissue and Cells are available. Please Contact us for more informationHuman Exosome Care Manual
Human Exosomes Publications
Bone Marrow Mesenchymal Stem Cell-Derived Exosome-Educated Macrophages Promote Functional Healing After Spinal Cord InjuryLi, Chengjun; Qin, Tian; Zhao, Jinyun; He, Rundong; Wen, Haicheng; et al.
Frontiers in Cellular Neuroscience; Lausanne (Sep 28, 2021). DOI:10.3389/fncel.2021.725573
Comprehensive Profiling of Plasma Exosomes Using Data-Independent Acquisitions-New Tools for Aging Cohort StudiesSandip K. Patel, Roland Bruderer, Nathan Basisty, Joanna Bons, Pierre-Yves Desprez, Francesco Neri, Lukas Reiter, Judith Campisi, Birgit Schilling
Acoustofluidic centrifuge for nanoparticle enrichment and separationYuyang Gu , Chuyi Chen, Zhangming Mao, Hunter Bachman, Ryan Becker, Joseph Rufo, Zeyu Wang, Peiran Zhang, John Mai, Shujie Yang, Jinxin Zhang, Shuaiguo Zhao, Yingshi Ouyang, David T. W. Wong, Yoel Sadovsky, Tony Jun Huang
Exosomeâeducated macrophages and exosomes differentially improve ligament healingConnie S. Chamberlain, John A. Kink, Linzie A. Wildenauer, Maxwell McCaughey, Katie Henry, Andrea M. Spiker, Matthew A. Halanski, Peiman Hematti, Ray Vanderby
Stem Cells. 2020;1–7. https://doi.org/10.1002/stem.3291
Macrophages Educated with Exosomes from Primed Mesenchymal Stem Cells Treat Acute Radiation Syndrome by Promoting Hematopoietic RecoveryJohn A. Kink, Matthew H. Forsberg, Sofiya Reshetylo, Soroush Besharat, Charlie J. Childs, Jessica D. Pederson, Annette Gendron-Fitzpatrick, Melissa Graham, Paul D. Bates, Eric G. Schmuck, Amish Raval, Peiman Hematti, Christian M. Capitini
Stem Cell-Derived Extracellular Vesicles Attenuate the Early Inflammatory Response after Tendon Injury and RepairHua Shen, Susumu Yoneda, Yousef AbuâAmer, Farshid Guilak, Richard H. Gelberman
Immunoassay for Detection of Virus-Antibody Nanocomplexes in Solution by Chip-Based Pillar ArrayUnited States Patent Application 20190107539
Extracellular vesicle-educated macrophages promote early Achilles tendon healingConnie S. Chamberlain, Anna B. Clements, John A. Kink, Ugeun Choi, Geoffrey S. Baer, Matthew A. Halanski, Peiman Hematti, and Ray Vanderby
Rapid Isolation and Detection of Exosomes and Associated Biomarkers from PlasmaStuart D. Ibsen, Jennifer Wright, Jean M. Lewis, Sejung Kim, Seo-Yeon Ko, Jiye Ong, Sareh Manouchehri, Ankit Vyas, Johnny Akers, Clark C. Chen, Bob S. Carter, Sadik C. Esener, and Michael J. Heller
Manufacturing of Human Extracellular Vesicle-Based Therapeutics for Clinical UseMario Gimona, Karin Pachler, Sandra Laner-Plamberger, Katharina Schallmoser and Eva Rohde
Int. J. Mol. Sci. 2017, 18(6), 1190; doi: 10.3390/ijms18061190
Surface Protein Characterization of Exosome Nanoparticles using a Multiplexing Approach
Nanoscale lateral displacement arrays for the separation of exosomes and colloids down to 20 nmBenjamin H. Wunsch, Joshua T. Smith, Stacey M. Gifford, Chao Wang, Markus Brink, Robert L. Bruce, Robert H. Austin, Gustavo Stolovitzky & Yann Astier
Nature Nanotechnology (2016) doi:10.1038/nnano.2016.134