Hepatic Cells
ZenBio offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Kupffers and Intra-hepatic biliary epithelial cells (IHBEC). We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. Human cells are isolated from whole liver tissue obtained via the gift of organ donation from donor tissue that is not suitable for organ transplantation and from living donor organ resections.
Cells have been tested for performance in viability. Cells have tested negative for HIV-1, HIV-2, HbsAG, HbcAB, HCV, and RPR. Cells are additionally tested for CMV. Some CMV positive lots are released for experiments.
Specially formulated media is available for the plating and maintenance of our cells.
Ordering Information:
Human Hepatocytes
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| HP-F | Cryopreserved Plateable Hepatocytes, minimum of 3 million cells/vial | Vial | $846.00 |
| HP-NP | Cryopreserved Mature Human Hepatocytes, Non-Plateable, Priced per million cells. Viable cell count varies per lot. Contact us for specific lots available and pricing | Vial | $141.00/million |
| HM-1 | Hepatocyte Plating Medium (expiry 30 days from ship date) | 500ml | $242.00 |
| HM-2 | Hepatocyte Maintenance Medium (expiry 30 days from ship date) | 500ml | $242.00 |
Human Hepatic Non-Parenchymal Cells
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| HP-F-S | Cryopreserved Human Hepatic Stellate Cells: 100,000 cells/vial. | Vial | $372.00 |
| HSGM-250 | Hepatic Stellate Growth Medium, 250ml | 250ml | $172.00 |
| HSGM-500 | Hepatic Stellate Growth Medium, 500ml | 500ml | $257.00 |
| HSGM-500-SF | Human Stellate Grown Medium, WITHOUT serum, 500ml | 500ml | $249.00 |
| HSFM-100 | Human Stellate Cryopreservation Medium, 100ml | 100ml | $215.00 |
| HP-KC-F | Cryopreserved Human Kupffer cells, min of 1 million cells/vial | Vial | $1,299.00 |
| KC-1 | Kupffer Cell Plating Medium, (HUMAN) 250ml | 250ml | $142.00 |
| KC-2 | Kupffer Cell Maintenance Medium, (HUMAN) 250ml | 250ml | $142.00 |
| HP-F-NPC | Cryoperserved Non-Parenchymal Cells (NPC), 1 million cells/vial | Vial | $261.00 |
| HP-NPC-250 | Hepatic Non-Parenchymal Cell Growth Medium | 250ml | $131.00 |
| HP-NPC-250-PRF | Hepatic Non-Parenchymal Cell Growth Medium, WITHOUT Phenol Red, | 250ml | $172.00 |
| HSEC-F | Cryopreserved Human Hepatic Sinusoidal Endothelial Cells, 500,000 cells/vial | Vial | $891.00 |
| ECGM-1 | Endothelial Growth Medium, 500ml | 500ml | $172.00 |
| IHBEC-F | Cryopreserved Human Intra-Hepatic Biliary Epithelial Cells: 500,000 cells/vial | Vial | $891.00 |
| IHBEC-1 | Intra-Hepatic Biliary Epithelial Cell Growth Medium, 500ml | 500ml | $186.00 |
Human Hepatic Progenitor Cells
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| HP-1 | Hepatic Progenitor Cell Plating Medium (expiry 30 days from ship date) | 500 ml | $441.00 |
| HP-2 | Hepatic Progenitor Cell Maintenance Medium (expiry 30 days from ship date) | 500 ml | $441.00 |
Non-Human Hepatocytes
| Item# | Item Desc | U/M | Price |
|---|---|---|---|
| BEA-F | Cryopreserved Plateable DOG (Beagle) Hepatocytes, min of 4 million cells/vial | Vial | $1261.00 |
| HM-3 | Rodent Hepatocyte Plating Medium (expiry 30 days from ship date) | 500ml | $242.00 |
| HM-4 | Rodent Hepatocyte Maintenance Medium (expiry 30 days from ship date) | 500ml | $242.00 |
| HM-5 | Dog Hepatocytes Plating Medium (expiry 30 days from ship date) | 500ml | $242.00 |
| HM-6 | Dog Hepatocytes Maintenance Medium (expiry 30 days from ship date) | 500ml | $242.00 |
For more information regarding our human liver products or to sign up for a HepAlert™ notification of new hepatocyte lots and updated product offerings Contact Us
To See the Hepatocyte Care and Maintenance Manual
A comprehensive list of products and prices can be found at Retail Prices(.PDF).
Hepatic Cells
Short chain fatty acids delay the development of hepatocellular carcinoma in HBx transgenic mice
Noreen McBrearty, Alla Arzumanyan, Eugene Bichenkov, Salim Merali, Carmen Merali, Mark Feitelsonhttps://doi.org/10.1016/j.neo.2021.04.004
Human Nonalcoholic Steatohepatitis on a Chip
May S. Freag, Bumseok Namgung, Maria E. Reyna Fernandez, Ermanno Gherardi, Shiladitya Sengupta, Hae Lin Janghttps://doi.org/10.1002/hep4.1647
Anti-fibrogenic effects of fatty acids in hepatic stellate cells
Siqi Hu, Minkyung Bae, Young-Ki Park, Ji-Young Leehttps://doi.org/10.1016/j.jnutbio.2020.108452
Astaxanthin inhibits the reduction of glycolysis during the activation of hepatic stellate cells
Minkyung Bae, Yoojin Lee, Tho X.Pham, Siqi Hu, Young-Ki Park, Ji-Young Leehttps://doi.org/10.1016/j.lfs.2020.117926
FBP1 loss disrupts liver metabolism and promotes tumorigenesis through a hepatic stellate cell senescence secretome
Fuming Li, Peiwei Huangyang, Michelle Burrows, Kathy Guo, Romain Riscal, Jason Godfrey, Kyoung Eun Lee, Nan Lin, Pearl Lee, Ian A. Blair, Brian Keith, Bo Li & M. Celeste SimonDOI://doi.org/10.1038/s41556-020-0511-2
Humanin attenuates palmitate-induced hepatic lipid accumulation and insulin resistance via AMPK-mediated suppression of the mTOR pathway
ChangHyuk Kwon, Jaw Long Sun, Ji Hoon Jeong, Tae Woo Junghttps://doi.org/10.1016/j.bbrc.2020.03.128
Transcriptome analysis of IPF fibroblastic foci identifies key pathways involved in fibrogenesis
Delphine Guillotin, Adam R. Taylor, Manuela Platé, Paul F. Mercer, Lindsay M. Edwards, Ross Haggart, Gino Miele, Robin J. McAnulty, Toby M. Maher, Robert E. Hynds, Mariam Jamal-Hanjani, Richard P. Marshall, Andrew J. Fisher, Andy D. Blanchard, and Rachel C. Chambersdoi: https://doi.org/10.1101/2020.03.10.984955.
SUMOylation inhibitors synergize with FXR agonists in combating liver fibrosis
Jiyu Zhou, Shuang Cui, Qingxian He, Yitong Guo, Xiaojie Pan, Pengfei Zhang, Ningning Huang, Chaoliang Ge, Guangji Wang, Frank J. Gonzalez, Hong Wang & Haiping HaoDOI https://doi.org/10.1038/s41467-019-14138-6
Therapeutic effects of a small molecule agonist of the relaxin receptor ML290 in liver fibrosis
Elena M. Kaftanovskaya, Hooi Hooi Ng, Mariluz Soula, Bryan Rivas, Courtney Myhr, Brian A. Ho, Briana A. Cervantes, Thomas D. Shupe, Mahesh Devarasetty, Xin Hu, Xin Xu, Samarjit Patnaik, Kenneth J. Wilson, Elena Barnaeva, Marc Ferrer, Noel T. Southall, Juan J. Marugan, Colin E. Bishop, Irina U. Agoulnik, and Alexander I. Agoulnik16 Aug 2019 https://doi.org/10.1096/fj.201901046R
Tumor necrosis factor-inducible gene 6 reprograms hepatic stellate cells into stem-like cells, which ameliorates liver damage in mouse
Sihyung Wang, Jieun Kim, Chanbin Lee, Dayoung Oh, Jinsol Han, Tae-Jin Kim, Sang-Woo Kim, Young-Su Seo, Seh-hoon Oh, Youngmi Junghttps://doi.org/10.1016/j.biomaterials.2019.119375
sGC stimulator praliciguat suppresses stellate cell fibrotic transformation and inhibits fibrosis and inflammation in models of NASH
Katherine C. Hall, Sylvie G. Bernier, Sarah Jacobson, Guang Liu, Ping Y. Zhang, Renee Sarno, Victoria Catanzano, Mark G. Currie, and Jaime L. MasferrerPNAS first published May 13, 2019 https://doi.org/10.1073/pnas.1821045116
Fucoxanthin exerts anti-fibrogenic effects in hepatic stellate cells
Mi-Bo Kim, Minkyung Bae, Siqi Hua, Hyunju Kang, Young-Ki Park, Ji-Young Leehttps://doi.org/10.1016/j.bbrc.2019.04.052
The mTORC1/4E-BP1 axis represents a critical signaling node during fibrogenesis
Hannah V. Woodcock, Jessica D. Eley, Delphine Guillotin, Manuela Platé, Carmel B. Nanthakumar, Matteo Martufi, Simon Peace, Gerard Joberty, Daniel Poeckel, Robert B. Good, Adam R. Taylor, Nico Zinn, Matthew Redding, Ellen J. Forty, Robert E. Hynds, Charles Swanton, Morten Karsdal, Toby M. Maher, Giovanna Bergamini, Richard P. Marshall, Andy D. Blanchard, Paul F. Mercer & Rachel C. ChambersDOI https://doi.org/10.1038/s41467-018-07858-8
Human liver spheroid cultures in microfluidic chip co-cultures and comparative MetID studies
JOHANNA ASSERLINDCHALMERS UNIVERSITY OF TECHNOLOGY
Maresin 1 attenuates NAFLD by suppression of endoplasmic reticulum stress via AMPK-SERCA2b pathway
Tae Woo Jung, Hyoung-Chun Kim, A.M. Abd El-Aty, Ji Hoon JeongThe latest version is at http://www.jbc.org/cgi/doi/10.1074/jbc.RA117.000885 JBC Papers in Press. Published on February 5, 2018 as Manuscript RA117.000885
WISP1 promotes nonâ€alcoholic fatty liver disease and skeletal muscle insulin resistance via TLR4/JNK signaling
Tae Woo Jung, Changmuk Kang, Jiwon Goh, Soo In Chae, Hyoung-Chun Kim, Tae Jin Lee, A. M. Abd El-Aty, Ji HoonDOI: 10.1002/jcp.26449
Coordinated regulation of hepatic FoxO1, PGC-1α and SREBP-1c facilitates insulin action and resistance
Mini P. Sajan, Mackenzie C. Lee, Fabienne Foufelle, Joshua Sajan, Courtney Cleland, Robert V. Faresehttps://doi.org/10.1016/j.cellsig.2017.12.005
Integrin alpha 11 in the regulation of the myofibroblast phenotype: implications for fibrotic diseases
Ruchi Bansal, Shigeki Nakagawa, Saleh Yazdani, Joop van Baarlen, Anu Venkatesh, Anna P Koh, Won-Min Song, Nicolas Goossens, Hideo Watanabe, Mary B Beasley, Charles A Powell, Gert Storm, Naftali Kaminski, Harry van Goor, Scott L Friedman, Yujin Hoshida & Jai Prakashdoi:10.1038/emm.2017.213
Hyperlipidemia-induced hepassocin in the liver contributes to insulin resistance in skeletal muscle
Tae WooJung,, Hyoung-ChunKim, A.M.AbdEl-Aty, Ji HoonJeonghttps://doi.org/10.1016/j.mce.2017.10.014
An In Vitro Human Liver Model by iPSC-Derived Parenchymal and Non-parenchymal Cells
Yuta Koui,Taketomo Kido, Toshimasa Ito, Hiroki Oyama, Shin-Wei Chen, Yuki Katou, Katsuhiko Shirahige, and Atsushi Miyajimahttp://dx.doi.org/10.1016/j.stemcr.2017.06.010
Microengineered cultures containing human hepatic stellate cells and hepatocytes for drug development
Matthew Davidson, David A Kukla and Salman KhetaniIntegr. Biol., 2017, DOI:10.1039/C7IB00027H
Protectin DX suppresses hepatic gluconeogenesis through AMPK-HO-1-mediated inhibition of ER stress
Tae Woo Junga, Hyung-Chun Kimb, Ji Hoon Jeongchttp://dx.doi.org/10.1016/j.cellsig.2017.03.013
In vitro toxicological evaluation of NCS-382, a high-affinity antagonist of γ-hydroxybutyrate (GHB) binding
KR Vogela, GR Ainsliea, J-B Roulleta, A McConnellb, KM Gibsonhttp://dx.doi.org/10.1016/j.tiv.2017.01.013
Inhibition of Adhesion Molecule Gene Expression and Cell Adhesion by the Metabolic Regulator PGC-1α
Neri Minsky, Robert G. Roederhttp://dx.doi.org/10.1371/journal.pone.0165598
Resveratrol exerts a biphasic effect on apolipoprotein M
Makoto Kurano, Masumi Hara, Takahiro Nojiri, Hitoshi Ikeda, Kazuhisa Tsukamoto and Yutaka YatomiDOI: 10.1111/bph.13360
C1q/TNF-Related Protein 9 (CTRP9) attenuates hepatic steatosis via the autophagy-mediated inhibition of endoplasmic reticulum stress
Tae Woo Jung, Ho Cheol Hong, Hwan-Jin Hwang, Hye Jin Yoo, Sei Hyun Baik, Kyung Mook Choidoi:10.1016/j.mce.2015.09.027
Gastrointestinal Fibroblasts Have Specialized, Diverse Transcriptional Phenotypes: A Comprehensive Gene Expression Analysis of Human Fibroblasts
Youichi Higuchi, Motohiro Kojima, Genichiro Ishii, Kazuhiko Aoyagi, Hiroki Sasaki, Atsushi OchiaiPublished: June 5, 2015DOI: 10.1371/journal.pone.0129241
Hepatic stellate cells express thymosin Beta 4 in chronically damaged liver.
Jieun Kim, Sihyung Wang, Jeongeun Hyun, Steve S. Choi, Heejae Cha, Meesun Ock, Youngmi JungPublished: March 31, 2015DOI: 10.1371/journal.pone.0122758
Preadipocytes Mediate Lipopolysaccharide-Induced Inflammation and Insulin Resistance in Primary Cultures of Newly Differentiated Human Adipocytes.
Soonkyu Chung, Kathleen LaPoint, Kristina Martinez, Arion Kennedy, Maria Boysen Sandberg, and Michael K. McIntosh Endocrinology, Nov 2006; 147: 5340 - 5351.Ritonavir, Saquinavir, and Efavirenz, but Not Nevirapine, Inhibit Bile Acid Transport in Human and Rat Hepatocytes.
Mary Peace McRae, Carolina M. Lowe, Xianbin Tian, David L. Bourdet, Richard H. Ho, Brenda F. Leake, Richard B. Kim, Kim L. R. Brouwer, and Angela D. M. Kashuba J. Pharmacol. Exp. Ther., Sep 2006; 318: 1068 - 1075.The Use of Whole Organ Decellularization for the Generation of a Vascularized Liver Organoid
Pedro M. Baptista,1* Mohummad M. Siddiqui,2* Genevieve Lozier,3 Sergio R. Rodriguez,1,4 Anthony Atala,1 and Shay SokerEssential Role of Endocytosis of the Type II Transmembrane Serine Protease TMPRSS6 in Regulating Its Functionality*
Francois Béliveau, Cédric Brulé, Antoine D&ecute;silets, Brandon Zimmerman, Stéphane A. Laporte Christine L. Lavoie and Richard Leduc August 19, 2011 The Journal of Biological Chemistry, 286,29035-29043Soluble CLEC2 Extracellular Domain Improves Glucose and Lipid Homeostasis by Regulating Liver Kupffer Cell Polarization
X Wu, J Zhang, H Ge, J Gupte, H Baribault, KJ Lee ...- EBioMedicine, 2015
