Support: Reagent Manuals

3T3-L1 Cryopreservation Medium (pdf)
FM-1-100 Medium manuals (pdf)
Dispase II (pdf)
Dispase II, a non-mammalian, partially purified, neutral protease isolated from culture filtrates of Bacillus polymyxa, has proven to be a rapid, effective, but gentle agent for tissue dissociation. This product is animal origin free. The mild pr oteolytic actions make this enzyme suitable for separating intact epidermis from the dermis and in tact epithelial sheets in culture from the substratum. Dispase cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. This protease is also used as a secondary enzyme in cell dissociation and tissue dissociation applications, commonly used with collagenase. Inhibitors include 1mM EDTA, EGTA, 1-10-phenanthroline, and heavy metals. This product is stable for 12 months upon arrival when stored at 2-8 °C.