Skeletal Myoblasts

Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes.

Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. Skeletal myoblasts are available from both male and female donors. Pooled lots of cells are available to provide large lots for screening and to decrease issues with patient to patient variability.

Cells are available cryopreserved (500,000 cells/vial).

QUALITY CONTROL: All cells have been tested for myotube differentiation. Cells have tested negative for HIV-1, HIV-2, HTLC-1, HTLV-2, Hepatitis B, Hepatitis C and mycoplasma. Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. Myogenic differentiation was verified by immunohistochemical staining using antibodies to desmin and myosin. The differentiated myocytes also respond to insulin stimulated glucose uptake.

Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts.

Skeletal Myocytes Skeletal Myocytes
Human skeletal myocytes in culture

Ordering Information:

Cryopreserved skeletal myoblasts

Item#Item DescU/MPrice
SKB-FCryopreserved Human Skeletal Myoblasts, (0.5 x 106 cells/vial)Vial$434.00
SKB-F-SLCryopreserved Human Skeletal Myoblasts, (0.5 x 106 cells/vial), POOLED donor lotVial$434.00
SKB-F-1Cryopreserved Human Skeletal Myoblasts, LEAN DONOR (BMI <25.0), (0.5 x 106 cells/vial)Vial$970.00
SKM-D-FCryopreserved Human Skeletal Myoblasts, DIABETIC Donor, (0.5 x 106 cells/vial)Vial$970.00

Skeletal Myoblast Media

Item#Item DescU/MPrice
SKM-DSkeletal Muscle Cell Differentiation Medium500ml$138.00
SKM-MSkeletal Muscle Cell Growth Medium500ml$138.00
SKM-50Skeletal Muscle Cell Growth Medium50ml$90.00
SKM-M-ABFSkeletal Muscle Cell Growth Medium, WITHOUT Antibiotics500ml$138.00
SKM-100Skeletal Muscle Cell Cryopreservation Medium100ml$183.00

Contact Us For More Information.

Human Skeletal Muscle Myoblast Manual (pdf)

A comprehensive list of products and prices can be found at Retail Prices(.PDF).

Recent Publications: Skeletal MyoblastsView
Use of deep neural network ensembles to identify embryonic-fetal transition markers: repression of COX7A1 in embryonic and cancer cells Michael D. West, Ivan Labat, Hal Sternberg, Dana Larocca, Igor Nasonkin, Karen B. Chapman, Ratnesh Singh, Eugene Makarev, Alex Aliper, Andrey Kazennov, Andrey Alekseenko, Nikolai Shuvalov, Evgenia Cheskidova, Aleksandr Alekseev, Artem Artemov, Evgeny Putin, Polina Mamoshina, Nikita Pryanichnikov, Jacob Larocca, Karen Copeland, Evgeny Izumchenko, Mikhail Korzinkin and Alex Zhavoronkov
Oncotarget. 2018; 9:7796-7811.
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Satellite Cell Activity and Self-Renewal in Patients with Cerebral Palsy Stephanie K Yeager
University of Delaware, ProQuest Dissertations Publishing, 2017. 10269621
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Association of cultured myotubes and fasting plasma metabolite profiles with mitochondrial dysfunction in Type 2 diabetes subjects Mohamad Hafizi Abu Bakar and Mohamad Roji Sarmidi
Mol. BioSyst., 2017, Accepted Manuscript , DOI:10.1039/C7MB00333A
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Glucose Sensing by Skeletal Myocytes Couples Nutrient Signaling to Systemic Homeostasis Zhuo-Xian Meng, Jianke Gong, Zhimin Chen, Jingxia Sun, Yuanyuan Xiao, Lin Wang, Yaqiang Li, Jianfeng Liu, X. Z. Shawn Xu, Jiandie D. Lin6,'Correspondence information about the author Jiandie D. Lin
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Retention of acetylcarnitine in chronic kidney disease causes insulin resistance in skeletal muscle Yasunori Miyamoto, Teruo Miyazaki, Akira Honda, Homare Shimohata, Kouichi Hirayama, Masaki Kobayashi
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Pask integrates hormonal signaling with histone modification via Wdr5 phosphorylation to drive myogenesis Chintan K Kikani Xiaoying Wu Litty Paul Hana Sabic Zuolian Shen Arvind Shakya Alexandra Keefe Claudio Villanueva Gabrielle Kardon Barbara Graves Dean Tantin Jared Rutter
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Jmjd2C increases MyoD transcriptional activity through inhibiting G9a-dependent MyoD degradation Eun-Shil Junga, Ye-Ji Sima, Hoe-Su Jeonga, Su-Jin Kima, Ye-Jin Yuna, Joo-Hoon Songb, Su-Hee Jeonc, Chungyoul Choed, Kyung-Tae Parke, Chang-Hoon Kima, , Kye-Seong Kim
Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, July 2015
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Celastrol Protects against Antimycin A-Induced Insulin Resistance in Human Skeletal Muscle Cells Mohamad Hafizi Abu Bakar , Kian-Kai Cheng , Mohamad Roji Sarmidi , Harisun Yaakob and Hasniza Zaman Huri
Molecules 2015, 20, 8242-8269; doi:10.3390/molecules20058242
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