Splenocytes - Splenocytes are dissociated into a single cell suspension so they can be easily manipulated ex-vivo. The splenocytes are isolated from excised spleens by mashing through a 60um screen, re-suspending in serum-free medium, and pelleting by centrifugation. Splenocytes are used for a variety of assays including T-cell activation, proliferation in response to mitogens, and cytokine production. Zen-bio offers both Human and Mouse splenocytes produced at Zen-Bio's facility from normal tissues. Each vial contains a minimum of 25 million viable cells.
Each lots is tested for viability and human donors are pathogen tested for HIV-1, HIV-2, HBV, HCV and Syphilis. Mouse cells are derived from clinically healthy animals.