Visceral Preadipocytes and Adipocytes
Metabolic disease is associated more closely with the accumulation of abdominal adipose in specific visceral depots. Recent studies have implicated omental and mesenteric adipose tissue in obesity-related insulin resistance, atherosclerosis, and type 2 diabetes. To provide the most relevant systems for investigating obesity-related morbidities, ZenBio is offering visceral derived human preadipocytes and cultured adipocytes to the research community.
Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.
Most cells are derived from obese non-diabetic patients (BMI>30), but there are limited quantities of preadipocytes and adipocytes from patients of BMI <27 as well as some lots of cells available from diabetic patients. Lots derived from multiple patients are also available as Pooled- or Super-lots.
There are limited quantities of donor matched omental and subcutaneous preadipocytes or cultured adipocytes for studies comparing the two depots.
The greater omentum is the largest peritoneal fold within the abdomen and has an immunologic function. The omentum aids in isolating peritoneal infection and absorbing contaminants through the mesothelial stomata. Adipokines and fatty acids from this depot have direct access to the liver through the portal circulation which may lead to hepatic dysfunction.
Mesenteric adipose lies within the thin layers of the peritoneal mesothelium connecting the small and large intestine. Adipose accumulation within this depot correlates with an increased risk of metabolic disease.
Perirenal adipose resides near the kidneys outside the peritoneal cavity. Fat from this depot has not been correlated with increased risk for metabolic disease.
Specially formulated media are available for use with our omental preadipocytes and adipocytes.
|OP-2006-3||Omental Preadipocytes 6-Well Plate, BMI >30.0||Each||$996.00|
|OP-2012-3||Omental Preadipocytes 12-Well Plate, BMI >30.0||Each||$806.00|
|OP-2024-3||Omental Preadipocytes 24-Well Plate, BMI >30.0||Each||$806.00|
|OP-2048-3||Omental Preadipocytes 48-Well Plate, BMI >30.0||Each||$806.00|
|OP-2096-3||Omental Preadipocytes 96-Well Plate, BMI >30.0||Each||$539.00|
|OP-F-1||Cryopreserved, Omental (1 million cells/vial), BMI <25.0||Vial||$415.00|
|OP-F-2||Cryopreserved, Omental (1 million cells/vial), BMI 25.0-29.9||Vial||$415.00|
|OP-F-3||Cryopreserved, Omental (1 million cells/vial), BMI >30.0||Vial||$415.00|
|OP-F-SL||Cryopreserved, Omental (1 million cells/vial), (POOLED Donor Lot)||Vial||$415.00|
|OPD-F||Cryopreserved, Omental (1 million cells/vial), DIABETIC||Vial||$622.00|
|OPD-F-3||Cryopreserved, Omental Preadipocytes, DIABETIC (1 million cells/vial), BMI >30.0||Vial||$603.00|
|OPD-F-SL||Cryopreserved, Omental (1 million cells/vial), DIABETIC, (POOLED Donor Lot)||Vial||$622.00|
|OA-1006-3||Omental Adipocytes 6-Well Plate, BMI >30.0||Each||$1,135.00|
|OA-1012-3||Omental Adipocytes 12-Well Plate, BMI >30.0||Each||$946.00|
|OA-1024-3||Omental Adipocytes 24-Well Plate, BMI >30.0||Each||$946.00|
|OA-1048-3||Omental Adipocytes 48-Well Plate, BMI >30.0||Each||$946.00|
|OA-1096-3||Omental Adipocytes 96-Well Plate, BMI >30.0||Each||$678.00|
Contact Us For More Information.
A comprehensive list of products and prices can be found at Retail Prices(.PDF).
|Recent Publications: General Visceral Adipocyte||View|
β-aminoisobutyric acid attenuates LPS-induced inflammation and insulin resistance in adipocytes through AMPK-mediated pathway
Tae Woo Jung, Hyung Sub Park, Geum Hee Choi, Daehwan Kim and Taeseung Lee
Effects of exosomes from LPS-activated macrophages on adipocyte gene expression, differentiation, and insulin-dependent glucose uptake
De Silva, N., Samblas, M., MartÃnez, J.A. et al. J Physiol Biochem (2018).
| Intrinsic Properties of Brown and White Adipocytes Have Differential Effects on Macrophage Inflammatory Responses Louisa Dowal, Pooja Parameswaran, Sarah Phat, Syamala Akella, Ishita Deb Majumdar, Jyoti Ranjan, Chahan Shah, Saie Mogre, Kalyani Guntur, Khampaseuth Thapa, Stephane Gesta, Vivek K Vishnudas, Niven R. Narain and Rangaprasad Sarangarajan Berg, LLC |
| Resistin exposure of human omental adipocytes affects transcriptional regulation processes involved in cellular differentiation and maintenance. Sotiriou, Alexandros |
University. Promotor(en): Ivonne Rietjens, co-promotor(en): Jacques Vervoort. - Wageningen : Wageningen University - ISBN 9789463430302 - 137 p
| Hydroxytyrosol, an ingredient of olive oil, reduces triglyceride accumulation and promotes lipolysis in human primary visceral adipocytes during differentiation Bruno Stefanon, Monica Colitti |
Exp Biol Med (Maywood) June 10, 2016 1535370216654226
| Chemokine profiles of human visceral adipocytes from cryopreserved preadipocytes: Neutrophil activation and induction of nuclear factor-kappa B repressing factor Rui Yamaguchia, b, Takatoshi Yamamotoa, Arisa Sakamotoa, Yasuji Ishimarua, Shinji Naraharaa, Hiroyuki Sugiuchia, Yasuo Yamaguchi |
| Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics Mary G. Johlfs, Priyatham Gorjala, Yasuyo Urasaki, Thuc T. Le , Ronald R. Fiscus |
Published: July 1, 2015DOI: 10.1371/journal.pone.0132105
| Effects of Two Different Rhodiola rosea Extracts on Primary Human Visceral Adipocytes Elena Pomari, Bruno Stefanon and Monica Colitti |
Molecules 2015, 20, 8409-8428; doi:10.3390/molecules20058409
| Inflammatory Cytokines Link Obesity and Breast Cancer Nalin Siriwardhana1, Rett Layman2, Ayub Karwandyar2, Shiwani Patel2, Blair Tage2, Matthew Clark2, Jessica Lampley2, Courtney Rhody2, Erica Smith2, Arnold M Saxton1, Naima Moustaid-Moussa1* and Jay Wimalasena2 |
Journal of Metabolic Syndrome, March 2015
|Circulating Pigment Epithelium-Derived Factor Levels Are Associated with Insulin Resistance and Decrease after Weight Loss. Sabater M, Moreno-Navarrete JM, Ortega FJ, Pardo G, Salvador J, Ricart W, Frühbeck G, Fernández-Real JM 2010 J Clin Endocrinol Metab 95:4720-4728.|
|Synthesis and Secretion of Plasminogen Activator Inhibitor-1 by Human Preadipocytes. David L. Crandall, Elaine M. Quinet, Gwen A. Morgan, Dennis E. Busler, Barbara McHendry-Rinde, and John G. Kral J. Clin. Endocrinol. Metab., Sep 1999; 84: 3222 - 3227.|
|Study of the proinflammatory role of human differentiated omental adipocytes. Bassols, J., Ortega, F. J., Moreno-Navarrete, J. M., Peral, B., Ricart, W. and Fernández-Real, J.-M. (2009), Journal of Cellular Biochemistry, 107: 110-1117. doi: 10.1002/jcb.22208.|
|LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes. Bassols J, Moreno-Navarrete JM, Ortega F, Ricart W, Fernandez-Real JM (2010) Int J Obes 34:146-156.|
|Characterization of herpes virus entry mediator as a factor linked to obesity. Bassols J, Moreno JM, Ortega F, Ricart W, Fernandez-Real JM (2010) Obesity 18(2): 239-46. doi:10.1038/oby.2009.250.|
|Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue Rafael Pérez-Pérez, Eva Garcia-Santos, Francisco J. Ortega-Delgado, Juan A. Lopez, Emilio Camafeita, Wifredo Ricart, José-Manuel FernÃ¡ndez-Real, Belén Peral.|
|METHODS AND MATERIALS RELATED TO NUTRITIONAL SUPPLEMENT COMPOSITIONS CONTAINING A POTATO POLYSACCHARIDE PREPARATION|