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Visceral Preadipocytes and Adipocytes

Metabolic disease is associated more closely with the accumulation of abdominal adipose in specific visceral depots. Recent studies have implicated omental and mesenteric adipose tissue in obesity-related insulin resistance, atherosclerosis, and type 2 diabetes. To provide the most relevant systems for investigating obesity-related morbidities, ZenBio is offering visceral derived human preadipocytes and cultured adipocytes to the research community.

Visceral preadipocytes

Preadipocytes are isolated from omental, mesenteric, or perirenal adipose tissue and are tested for their ability to differentiate in culture to mature adipocytes. Preadipocytes are available cryopreserved or plated and mature adipocytes are available in a variety of plated formats.

Most cells are derived from obese non-diabetic patients (BMI>30), but there are limited quantities of preadipocytes and adipocytes from patients of BMI <27 as well as some lots of cells available from diabetic patients. Lots derived from multiple patients are also available as Pooled- or Super-lots.

There are limited quantities of donor matched omental and subcutaneous preadipocytes or cultured adipocytes for studies comparing the two depots.

OMENTAL ADIPOSE

The greater omentum is the largest peritoneal fold within the abdomen and has an immunologic function. The omentum aids in isolating peritoneal infection and absorbing contaminants through the mesothelial stomata. Adipokines and fatty acids from this depot have direct access to the liver through the portal circulation which may lead to hepatic dysfunction.

MESENTERIC ADIPOSE

Mesenteric adipose lies within the thin layers of the peritoneal mesothelium connecting the small and large intestine. Adipose accumulation within this depot correlates with an increased risk of metabolic disease.

PERIRENAL ADIPOSE

Perirenal adipose resides near the kidneys outside the peritoneal cavity. Fat from this depot has not been correlated with increased risk for metabolic disease.

Specially formulated media are available for use with our omental preadipocytes and adipocytes.

Ordering Information:

Preadipocytes

Item#Item DescU/MPrice
OP-2006-3Omental Preadipocytes 6-Well Plate, BMI >30.0Each$966.00
OP-2012-3Omental Preadipocytes 12-Well Plate, BMI >30.0Each$782.00
OP-2024-3Omental Preadipocytes 24-Well Plate, BMI >30.0Each$782.00
OP-2048-3Omental Preadipocytes 48-Well Plate, BMI >30.0Each$782.00
OP-2096-3Omental Preadipocytes 96-Well Plate, BMI >30.0Each$523.00
OP-25-3Omental Preadipocytes 25 cm2 Flask, BMI >30.0Each$523.00
OP-75-3Omental Preadipocytes 75 cm2 Flask, BMI >30.0Each$1,365.00
OP-F-1Cryopreserved, Omental (1 million cells/vial), BMI <25.0Vial$402.00
OP-F-2Cryopreserved, Omental (1 million cells/vial), BMI 25.0-29.9Vial$402.00
OP-F-3Cryopreserved, Omental (1 million cells/vial), BMI >30.0Vial$402.00
OP-F-SLCryopreserved, Omental (1 million cells/vial), (POOLED Donor Lot)Vial$402.00
OPD-FCryopreserved, Omental (1 million cells/vial), DIABETICVial$603.00
OPD-F-3Cryopreserved, Omental Preadipocytes, DIABETIC (1 million cells/vial), BMI >30.0Vial$603.00
OPD-F-SLCryopreserved, Omental (1 million cells/vial), DIABETIC, (POOLED Donor Lot)Vial$603.00
MS-F-3Matched Set Subcutaneous and Omental, Cryopreserved, (1 vial of each), BMI >302 Vials$1,101.00

Item#Item DescU/MPrice
PR-FCryopreserved Peri-Renal Preadipocytes, (1.0x106 cells/vial)Vial$424.00
MSN-FCryopreserved Mesenteric Preadipocytes (1.0x106 cells/vial)Vial$369.00
MSND-FCryopreserved Mesenteric Preadipocytes, DIABETIC (1.0x106 cells/vial)Vial$693.00

Adipocytes

Item#Item DescU/MPrice
OA-1006-3Omental Adipocytes 6-Well Plate, BMI >30.0Each$1,101.00
OA-1012-3Omental Adipocytes 12-Well Plate, BMI >30.0Each$918.00
OA-1024-3Omental Adipocytes 24-Well Plate, BMI >30.0Each$918.00
OA-1048-3Omental Adipocytes 48-Well Plate, BMI >30.0Each$918.00
OA-1096-3Omental Adipocytes 96-Well Plate, BMI >30.0Each$658.00
OA-25Omental Adipocytes 25 cm2 Flask, BMI >30.0Each$625.00
OA-75Omental Adipocytes 75 cm2 Flask, BMI >30.0Each$1,297.00

Visceral Omental Adipocyte Care Manual

Contact Us For More Information.

A comprehensive list of products and prices can be found at Retail Prices(.PDF).

Recent Publications: General Visceral AdipocyteView
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Intrinsic Properties of Brown and White Adipocytes Have Differential Effects on Macrophage Inflammatory Responses Louisa Dowal, Pooja Parameswaran, Sarah Phat, Syamala Akella, Ishita Deb Majumdar, Jyoti Ranjan, Chahan Shah, Saie Mogre, Kalyani Guntur, Khampaseuth Thapa, Stephane Gesta, Vivek K Vishnudas, Niven R. Narain and Rangaprasad Sarangarajan Berg, LLC
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Resistin exposure of human omental adipocytes affects transcriptional regulation processes involved in cellular differentiation and maintenance. Sotiriou, Alexandros
University. Promotor(en): Ivonne Rietjens, co-promotor(en): Jacques Vervoort. - Wageningen : Wageningen University - ISBN 9789463430302 - 137 p
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Hydroxytyrosol, an ingredient of olive oil, reduces triglyceride accumulation and promotes lipolysis in human primary visceral adipocytes during differentiation Bruno Stefanon, Monica Colitti
Exp Biol Med (Maywood) June 10, 2016 1535370216654226
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Chemokine profiles of human visceral adipocytes from cryopreserved preadipocytes: Neutrophil activation and induction of nuclear factor-kappa B repressing factor Rui Yamaguchia, b, Takatoshi Yamamotoa, Arisa Sakamotoa, Yasuji Ishimarua, Shinji Naraharaa, Hiroyuki Sugiuchia, Yasuo Yamaguchi
doi:10.1016/j.lfs.2015.11.010, LifeSciences
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Capillary Isoelectric Focusing Immunoassay for Fat Cell Differentiation Proteomics Mary G. Johlfs, Priyatham Gorjala, Yasuyo Urasaki, Thuc T. Le , Ronald R. Fiscus
Published: July 1, 2015DOI: 10.1371/journal.pone.0132105
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Effects of Two Different Rhodiola rosea Extracts on Primary Human Visceral Adipocytes Elena Pomari, Bruno Stefanon and Monica Colitti
Molecules 2015, 20, 8409-8428; doi:10.3390/molecules20058409
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Inflammatory Cytokines Link Obesity and Breast Cancer Nalin Siriwardhana1, Rett Layman2, Ayub Karwandyar2, Shiwani Patel2, Blair Tage2, Matthew Clark2, Jessica Lampley2, Courtney Rhody2, Erica Smith2, Arnold M Saxton1, Naima Moustaid-Moussa1* and Jay Wimalasena2
Journal of Metabolic Syndrome, March 2015
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Circulating Pigment Epithelium-Derived Factor Levels Are Associated with Insulin Resistance and Decrease after Weight Loss. Sabater M, Moreno-Navarrete JM, Ortega FJ, Pardo G, Salvador J, Ricart W, Frühbeck G, Fernández-Real JM 2010 J Clin Endocrinol Metab 95:4720-4728. download pdf
Recent Publications: PreadipocyteView
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Synthesis and Secretion of Plasminogen Activator Inhibitor-1 by Human Preadipocytes. David L. Crandall, Elaine M. Quinet, Gwen A. Morgan, Dennis E. Busler, Barbara McHendry-Rinde, and John G. Kral J. Clin. Endocrinol. Metab., Sep 1999; 84: 3222 - 3227. download pdf
Study of the proinflammatory role of human differentiated omental adipocytes. Bassols, J., Ortega, F. J., Moreno-Navarrete, J. M., Peral, B., Ricart, W. and Fernández-Real, J.-M. (2009), Journal of Cellular Biochemistry, 107: 110-1117. doi: 10.1002/jcb.22208. download pdf
LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes. Bassols J, Moreno-Navarrete JM, Ortega F, Ricart W, Fernandez-Real JM (2010) Int J Obes 34:146-156. download pdf
Characterization of herpes virus entry mediator as a factor linked to obesity. Bassols J, Moreno JM, Ortega F, Ricart W, Fernandez-Real JM (2010) Obesity 18(2): 239-46. doi:10.1038/oby.2009.250. download pdf
Attenuated metabolism is a hallmark of obesity as revealed by comparative proteomic analysis of human omental adipose tissue Rafael Pérez-Pérez, Eva Garcia-Santos, Francisco J. Ortega-Delgado, Juan A. Lopez, Emilio Camafeita, Wifredo Ricart, José-Manuel Fernández-Real, Belén Peral. download pdf
METHODS AND MATERIALS RELATED TO NUTRITIONAL SUPPLEMENT COMPOSITIONS CONTAINING A POTATO POLYSACCHARIDE PREPARATION download pdf
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