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Lipolysis Kits

Lipoysis Kits

Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides (TG) are hydrolyzed into glycerol and free fatty acids. This process releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Excessive free fatty acid production is believed to contribute to insulin resistance in skeletal muscle that is observed in obesity. Hormone sensitive lipase is the rate-limiting enzyme catalyzing triglyceride breakdown. Perilipins, one of the PAT (perilipins, adipophilin, TIP47 proteins) family of lipid-associated proteins, are implicated in adipocyte lipolysis by mediating the interaction of HSL with the triacylglycerol molecule (Brasaemle et al. 2004; reviewed in, Tansey et al. 2004.) The presence of these proteins corresponds to lipolytic stimulation in cultured adipocytes (Braemle et al. 2004).

The sympathetic nervous system also plays a key role in the regulation of lipid mobilization. The main lipolytic pathway involves beta-agonists (β-agonists), which activate β-adrenergic receptors via the intracellular Gs proteins in adipocytes. This leads to the activation of adenylate cyclase (AC), which then increases cyclic AMP (cAMP) levels. Elevated cAMP acts as a second messenger to activate hormone sensitive lipase (HSL). HSL, the rate-limiting enzyme regulating adipocyte lipolysis, then catalyzes the hydrolysis of triglycerides and results in the release of glycerol and FFA (increased lipolysis). Phosphodiesterases (PDE) are enzymes that hydrolyze cAMP to 5'-AMP (5 prime adenosine monophosphate). This action results in a decrease in lipolysis. PDE inhibitors increase intracellular cAMP levels. 3-isobutyl-1-methylxanthine (IBMX), a non-specific inhibitor of cAMP phosphodiesterases (PDE), is used as the positive control if your test compounds are suspected PDE inhibitors. Isoproterenol, a non-specific β-adrenergic agonist is used as the positive control if your test compounds affect lipolysis via β-adrenergic receptors (Robidoux et al. 2004).

Our lipolysis assay kits provide the tool to study chemical compounds that may influence lipolysis in cultured human or 3T3-L1 adipocytes. Kits are available with and without a plate of cells. Kits can measure the glycerol and/or free fatty acids released in lipolysis.

Overview of adipocyte lipolysis


lipolysis addipocyte

Ordering Information:

Human Lipolysis Assay Kits

Item#Item DescU/MPrice
LIP-1Lipolysis Assay KIT, Glycerol Detection ( REAGENTS+ CELLS)KIT$731.00
LIP-1-NCLipolysis Assay KIT , Glycerol Detection (REAGENTS ONLY)KIT$348.00
LIP-1-RBLipolysis Assay KIT (5 plates; REAGENTS+ BUFFERS ONLY)KIT$1,4629.00
LIP-1-SPFLipolysis Assay Kit, Glycerol Detection, WITH Cryopreserved Subcutaneous Preadipocytes (2 million cells)KIT$603.00
LIP-2Lipolysis Assay KIT, Free fatty acids Detection (REAGENTS+ CELLS)KIT$826.00
LIP-2-NCLipolysis Assay KIT, Free fatty acids Detection (REAGENTS ONLY)KIT$444.00
LIP-2-RBLipolysis Assay KIT, Free Fatty Acids Detection (5 plates; REAGENTS+ BUFFERS ONLY)KIT$1,876.00
LIP-2-SCRNLipolysis Assay KIT, Free Fatty Acids Detection-Automation Screening KIT, REAGENTS ONLYKIT$3,025.00
LIP-3Lipolysis Assay KIT Dual glycerol and free fatty acids Detection (REAGENTS+ CELLS)KIT$1,006.00
LIP-3-NCLipolysis Assay KIT , Dual Glycerol and free fatty acids Detection (REAGENTS ONLY)KIT$628.00
LIP-3-RBLipolysis Assay KIT, Dual Glycerol and free fatty acids Detection (REAGENTS ONLY for 5-96 well plates)KIT$2,669.00
LIP-3-OMLipolysis Assay KIT, Dual Glycerol and free fatty acids Detection, Omental Cells (REAGENTS+ CELLS), 100-pointsKIT$1,107.00
LIP-6-NCLipolysis Assay Kit, Adipose Tissue Explant: Glycerol Detection (REAGENTS ONLY)KIT$348.00
LIP-GLYSTANGlycerol Standard, for Lipolysis Assay KitsEACH$34.00
RGTA-1011-ML Glycerol Reagent AEACH$91.00
RGTA-4040-ML Glycerol Reagent AEACH$144.00

3T3-L1

Item#Item DescU/MPrice
LIP-1-L13T3-L1 Lipolysis Assay Kit (glycerol detection; REAGENTS+ CELLS)*Kit$667.00
LIP-1-L1-F3T3-L1 Lipolysis Assay Kit (glycerol detection; REAGENTS+ VIAL of CRYOPRESERVED CELLS)Kit$614.00
LIP-1-NCL1DIF3T3-L1 Lipolysis Assay Kit (glycerol detection; REAGENTS+ MEDIA) Includes media to differentiate 1 x96 well plateKit$459.00
LIP-1-NC-L13T3-L1 Lipolysis Assay Kit (glycerol detection; REAGENTS ONLY)Kit$348.00
LIP-2-L13T3-L1 Lipolysis Assay Kit (free fatty acid detection; REAGENTS+ CELLS)*Kit$763.00
LIP-2-NC-L13T3-L1 Lipolysis Assay Kit (free fatty acids detection; REAGENTS ONLY)Kit$444.00
LIP-3-L13T3-L1 Lipolysis Assay Kit (dual glycerol and free fatty acids detection; REAGENTS+ CELLS)*Kit$891.00
LIP-3-NCL13T3-L1 Lipolysis Assay Kit (dual glycerol and free fatty acids detection; REAGENTS ONLY)Kit$628.00

* This kit includes one 96-well plate of 3T3-L1 preadipocytes, media to differentiate the cells and reagents for the detection of lipolysis.

To see kit manuals

ZenBio Kits - Recent Publications

LIP-1

  1. LIP-1-NC lipolysis kit used to quantify glycerol release in hormone stimulated adipocytes (Gagnon, et al., 2010).
  2. Lipolysis Assay kit used in 3T3L-1 cells and primary adipocytes (Liu, et al., 2009).
  3. Lipolysis Assay Kit Buffer used to measure adipogenesis regulation by G protein coupled receptor 103 (Mulumba, et al.).
  4. Lipolysis Assay kit used to measure lipolysis in 3T3 Cells after caveloin-1 knockdown by siRNA (Ahmad, et al., 2009).
  5. Glycerol Reagent (Lipolysis kit) used to measure lipolysis in 3t3-1 adipocytes following Xanthohumol and guggulsterone treatment (Rayalam, et al., 2009).
  6. Lipolysis Kit used to measure the glycerol content in the culture medium after isoproterenol treatment (Klein, Schreml, Nerlich, & Prantl, 2009).
  7. Lipolysis Kit used to measure lipolysis in primary murine adipocytes from differentiated white fat precursor cells (Hiroyama, et al., 2009).
  8. Lipolysis Kit used to measure lipolysis after treatment of primary adipocytes with isoproterenol or recombinant leptin (Cernkovich, Deng, Bond, Combs, & Harp, 2008).
  9. Glycerol reagent (lipolysis kit) used to measure lipolysis change in cells after Guggulsterone treatment (Yang, Della-Fera, & Baile, 2008).
  10. Glycerol reagent (lipolysis kit) used to measure change in triglyceride hydrolysis following injection of antimicrobial peptide dermicidin in vivo (K.-A. Kim, et al., 2008).
  11. Lipolysis assay kit used to measure FFA released from mature adipocytes and media from breast cancer cells (J. H. Kim, et al., 2008).
  12. Lipolysis assay kit used in adipose-derived stem cells aging study (Schipper, Marra, Zhang, Donnenberg, & Rubin, 2008).

LIP-2

  1. FFA kit used in patients for angiopoietin study (Robciuc, Tahvanainen, Jauhiainen, & Ehnholm, 2010).
  2. FFA kit used in liver steatosis study in SIRT1 knockout mice (Xu, et al., 2010).
  3. FFA kit used in an oxidative stress study to determine the interaction between dyslipidemia and inflammation in obese children (Oliver, et al., 2010).
  4. FFA kit used to support circadian rhythm analysis in rat adipose tissue (Sukumaran, Xue, Jusko, Dubois, & Almon, 2010).
  5. FFA kit used to measure FFA after diet restriction in two rock climbers, oxidative stress study (Merrells, Friel, Knaus, & Suh, 2008).

LIP-3

  1. TG/FFA kit used to measure regulation of serum lipids by Pck1 in insulin resistant mice (Millward, et al., 2010).
  2. TG/FFA kit used to measure lipolysis and triglyceride accumulation in adipocytes after treatment with all-trans retinoic acid (Berry, Soltanian, & Noy, 2010).
  3. TG/FFA kit used to measure triglycerides and lipolysis in 3T3-L1 cells after treatment with dexamethasone and methylisobutylxanthine (Pantoja, Huff, & Yamamoto, 2008).

LIP-1-LI

  1. Adipocyte lipolysis assay kit use to measure white adipocyte lipolysis activation (An, et al., 2010).
  2. 3T3-L1 adipocytes triglyceride lipolysis kit used to measure PPAR-Gamma effects on lipid storage (Lobo, et al., 2010).
  3. 3T3-L1 Adipocyte kit used to prepare cells for mineralcorticoid receptor blockade experiments (Guo, et al., 2008).
Recent Publications: View
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Human ADMC-Derived Adipocyte Thermogenic Capacity Is Regulated by IL-4 Receptor Fernando Lizcano, Diana Vargas, Ángela Gómez, and Astrid Torrado
https://doi.org/10.1155/2017/2767916
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Role of sulfotransferases in resveratrol metabolism in human adipocytes Nele Gheldof, Sofia Moco, Christian Chabert, Tony Teav, Denis Barron, Jorg Hager
DOI: 10.1002/mnfr.201700020
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Specific macronutrients exert unique influences on the adipose-liver axis to promote hepatic steatosis in mice Duwaerts CC, Amin AM, Siao K, Her C, Fitch M, Beysen C, Turner SM, Goodsell A, Baron JL, Grenert JP, Cho S-J, Maher JJ
Cellular and Molecular Gastroenterology and Hepatology (2017), doi: 10.1016/j.jcmgh.2017.04.004
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The role of moderate-to-vigorous physical activity in mediating the relationship between central adiposity and immunometabolic profile in postmenopausal women Tiego A. Diniz, Fabricio E. Rossi, Loreana S. Silveira, Lucas Melo Neves, Ana Claudia de Souza Fortaleza, Diego G. D. Christofaro, Fabio S. Lira, Ismael F. Freitas Junior
http://dx.doi.org/10.1590/2359-3997000000259
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Mfn2 is critical for brown adipose tissue thermogenic function Marie Boutant, Sameer S Kulkarni, Magali Joffraud, Joanna Ratajczak, Miriam Valera-Alberni, Roy Combe, Antonio Zorzano and Carles Cant´o;
DOI 10.15252/embj.201694914 | Published online 27.03.2017
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Adipocyte arrestin domain-containing 3 protein (Arrdc3) regulates uncoupling protein 1 (Ucp1) expression in white adipose independently of canonical changes in β- ... Shannon H. Carroll, Ellen Zhang, Bing F. Wang, Katherine B. LeClair, Arifeen Rahman, David E. Cohen, Jorge Plutzky, Parth Patwari, Richard T. Lee
http://dx.doi.org/10.1371/journal.pone.0173823
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Biological activities of the legume crops Melilotus officinalis and Lespedeza capitata for skin care and pharmaceutical applications Giulia Pastorinoa, Carla Marchettib, Barbara Borghesia, Laura Cornaraa, Stefania Ribullac, Bruno Burlandob,
http://dx.doi.org/10.1016/j.indcrop.2016.11.047
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Whole-exome sequencing identifies ADRA2A mutation in atypical familial partial lipodystrophy Abhimanyu Garg,1 Shireesha Sankella,1 Chao Xing,2 and Anil K. Agarwal
JCI Insight. 2016;1(9):e86870. doi:10.1172/jci.insight.86870
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The ubiquitin ligase Siah2 regulates obesity-induced adipose tissue inflammation Gail Kilroy, Lauren E. Carter, Susan Newman, David H. Burk, Justin Manuel, Andreas Möller, David D. Bowtell, Randall L. Mynatt, Sujoy Ghosh, and Z. Elizabeth Floyd
Article first published online: 18 SEP 2015, OBESITY
DOI: 10.1002/oby.21220
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Tissue Inhibitor Of Matrix Metalloproteinase-1 Is Required for High-Fat Diet-Induced Glucose Intolerance and Hepatic Steatosis in Mice Even Fjære, Charlotte Andersen, Lene Secher Myrmel, Rasmus Koefoed Petersen, Jakob Bondo Hansen, Hanne Sørup Tastesen, Thomas Mandrup-Poulsen, Nils Brünner, Karsten Kristiansen, Lise Madsen, Maria Unni Rømer
DOI: 10.1371/journal.pone.0132910
Age-related lipid metabolic signature in human LMNA-lipodystrophic stem cell derived adipocytes Patricia Sánchez, Arantza Infante, Garbiñe Ruiz de Eguino, Jorge A. Fuentes-Maestre, José Manuel García-Verdugo, and Clara I. Rodríguez
doi: 10.1210/jc.2014-4528, J Clin Endocrinol Metab
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Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes H Zhi, L Qu, F Wu, L Chen, J Tao - Obesity, 20159 MAR 2015 DOI: 10.1002/oby.21015 download pdf
Hypermetabolism of Fat in V1a Vasopressin Receptor Knockout Mice. Masami Hiroyama, Toshinori Aoyagi, Yoko Fujiwara, Junichi Birumachi, Yosuke Shigematsu, Kohji Kiwaki, Ryuji Tasaki, Fumio Endo, and Akito Tanoue Mol. Endocrinol., Jan 2007; 21: 247 - 258. download pdf
Thrombin increases inflammatory cytokine and angiogenic growth factor secretion in human adipose cells in vitro. J. L. Strande and S. A. Phillips J Inflamm (Lond). 2009; 6 4. download pdf
Monoclonal antibody antagonists of hypothalamic FGFR1 cause potent but reversible hypophagia and weight loss in rodents and monkeys. Haijun D. Sun, Maria Malabunga, James R. Tonra, Roberto DiRenzo, Francine E. Carrick, Huiyuan Zheng, Hans-Rudolf Berthoud, Owen P. McGuinness, Juqun Shen, Peter Bohlen, Rudolph L. Leibel, and Paul Kussie Am J Physiol Endocrinol Metab, Mar 2007; 292: E964 - E976. download pdf
Recent Publications: LIP-1 View
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Adipocyte culture medium stimulates production of macrophage inhibitory cytokine 1 in MDA-MB-231 cells. Kim, Jae Hyeong, Kim, Kun-yong, Jeon, Jun Ho, Lee, Su Hee, Hwang, Ji-Eun, Lee, Jung Hyeong, et al. 2008. Cancer letters; 261: 253-262. download pdf
Adipose-specific disruption of signal transducer and activator of transcription 3 increases body weight and adiposity. Cernkovich, Erin R, Deng, Jianbei, Bond, Michael C, Combs, Terry P, & Harp, Joyce B 2008. Endocrinology; 149: 1581-1590. download pdf
Altered lipid metabolism in vasopressin V1B receptor-deficient mice. Hiroyama, M., Fujiwara, Y., Nakamura, K., Aoyagi, T., Mizutani, R., Sanbe, A., et al. 2009. Eur J Pharmacol; 602: 455-461. download pdf
Anti-obesity effects of xanthohumol plus guggulsterone in 3T3-L1 adipocytes. Rayalam, Srujana, Yang, Jeong-Yeh, Della-Fera, Mary Anne, Park, Hea Jin, Ambati, Suresh, & Baile, Clifton A 2009. Journal of medicinal food; 12: 846-853. download pdf
Differential regulation of adipocyte PDE3B in distinct membrane compartments by insulin and the beta3-adrenergic receptor agonist CL316243: effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes. Ahmad, F., Lindh, R., Tang, Y., Ruishalme, I., Ost, A., Sahachartsiri, B., et al. 2009. Biochem J; 424: 399-410. download pdf
Effect of dermcidin, an antimicrobial peptide, on body fat mobilization in normal mice. Kim, Kyung-Ah, Ka, Sun-O, Moon, Woo Sung, Yi, Ho-Keun, Lee, Young-Hoon, Kwon, Kang-Beom, et al. 2008. The Journal of endocrinology; 198: 111-118. download pdf
GPR103b functions in the peripheral regulation of adipogenesis. Mulumba, M., Jossart, C., Granata, R., Gallo, D., Escher, E., Ghigo, E., et al. Mol Endocrinol; 24: 1615-1625. download pdf
Guggulsterone inhibits adipocyte differentiation and induces apoptosis in 3T3-L1 cells. Yang, Jeong-Yeh, Della-Fera, Mary Anne, & Baile, Clifton A 2008. Obesity (Silver Spring, Md.); 16: 16-22. download pdf
In vitro studies investigating the effect of subcutaneous phosphatidylcholine injections in the 3T3-L1 adipocyte model: lipolysis or lipid dissolution? Klein, S. M., Schreml, S., Nerlich, M., & Prantl, L. 2009. Plast Reconstr Surg; 124: 419-427. download pdf
Lactate inhibits lipolysis in fat cells through activation of an orphan G-protein-coupled receptor, GPR81. Liu, C., Wu, J., Zhu, J., Kuei, C., Yu, J., Shelton, J., et al. 2009. J Biol Chem; 284: 2811-2822. download pdf
Regional anatomic and age effects on cell function of human adipose-derived stem cells. Schipper, B. M., Marra, K. G., Zhang, W., Donnenberg, A. D., & Rubin, J. P. 2008. Ann Plast Surg; 60: 538-544. download pdf
Thyroid-stimulating hormone stimulates lipolysis in adipocytes in culture and raises serum free fatty acid levels in vivo. Gagnon, Annemarie, Antunes, Tayze T, Ly, Tapraya, Pongsuwan, Patama, Gavin, Claire, Lochnan, Heather A, et al. 2010. Metabolism: clinical and experimental; 59: 547-553. download pdf
Recent Publications: LIP-1-L1 View
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Beta-carotene decreases peroxisome proliferator receptor gamma activity and reduces lipid storage capacity of adipocytes in a beta-carotene oxygenase 1-dependent manner. Lobo, G.P.; Amengual, J.; Li, H.N.; Golczak, M.; Bonet, M.L.; Palczewski, K.; von Lintig, J. J. Biol. Chem. 2010, 285, 27891-27899. download pdf
Ethanolic extracts of Brassica campestris spp. rapa roots prevent high-fat diet-induced obesity via beta(3)-adrenergic regulation of white adipocyte lipolytic activity. An, Sojin, Han, Jang-Il, Kim, Min-Jung, Park, Ji-Seon, Han, Jong-Min, Baek, Nam-In, et al. 2010. Journal of medicinal food; 13: 406-414. download pdf
Mineralocorticoid receptor blockade reverses obesity-related changes in expression of adiponectin, peroxisome proliferator-activated receptor-gamma, and proinflammatory adipokines. Guo, Christine, Ricchiuti, Vincent, Lian, Bill Q, Yao, Tham M, Coutinho, Patricia, Romero, José R, et al. 2008. Circulation; 117: 2253-2261. download pdf
Recent Publications: LIP-2 View
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CIRCADIAN VARIATIONS IN GENE EXPRESSION IN RAT ABDOMINAL ADIPOSE TISSUE AND RELATIONSHIP TO PHYSIOLOGY. Sukumaran, Siddharth, Xue, Bai, Jusko, William J, Dubois, Debra C, & Almon, Richard R 2010. Physiological genomics; 42A: 141-152. download pdf
Following 2 diet-restricted male outdoor rock climbers: impact on oxidative stress and improvements in markers of cardiovascular risk. Merrells, K. J., Friel, J. K., Knaus, M., & Suh, M. 2008. Appl Physiol Nutr Metab; 33: 1250-1256. download pdf
Increased oxidative stress and altered substrate metabolism in obese children. Oliver, Stacy R, Rosa, Jaime S, Milne, Ginger L, Pontello, Andria M, Borntrager, Holly L, Heydari, Shirin, et al. 2010. International journal of pediatric obesity : IJPO : an official journal of the International Association for the Study of Obesity; 5: 436-444. download pdf
Lack of SIRT1 (Mammalian Sirtuin 1) activity leads to liver steatosis in the SIRT1+/- mice: a role of lipid mobilization and inflammation. Xu, Fen, Gao, Zhanguo, Zhang, Jin, Rivera, Chantal A, Yin, Jun, Weng, Jianping, et al. 2010. Endocrinology; 151: 2504-2514. download pdf
Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample. Robciuc, Marius R, Tahvanainen, Esa, Jauhiainen, Matti, & Ehnholm, Christian 2010. Journal of lipid research; 51: 824-831. download pdf
Recent Publications: LIP-3 View
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Glucocorticoid signaling defines a novel commitment state during adipogenesis in vitro. Pantoja, Carlos, Huff, Jason T, & Yamamoto, Keith R 2008. Molecular biology of the cell; 19: 4032-4041. download pdf
Phosphoenolpyruvate carboxykinase (Pck1) helps regulate the triglyceride/fatty acid cycle and development of insulin resistance in mice. Millward, Carrie A, Desantis, David, Hsieh, Chang-Wen, Heaney, Jason D, Pisano, Sorana, Olswang, Yael, et al. 2010. Journal of lipid research; 51: 1452-1463. download pdf
Repression of cellular retinoic acid-binding protein II during adipocyte differentiation. Berry, Daniel C, Soltanian, Hooman, & Noy, Noa 2010. The Journal of biological chemistry; 285: 15324-15332. download pdf
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