Subcutaneous Preadipocyte and Adipocyte Media

All ZenBio media products are ready-to-use when purchased. No mixing of growth factors or antibiotics is necessary. All media products are available as phenol-red free, serum-free, or specifically customized to meet your research needs.

Subcutaneous basal medium

Basal medium is serum-free and contains essential additives to maintain subcutaneous cells for up to 3 days. It can be supplemented for specific research purposes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous preadipocyte medium

Preadipocyte medium is used to plate preadipocytes prior to differentiation and contains growth factors necessary for expansion of subcutaneous preadipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous differentiation medium

Differentiation medium is used to differentiate subcutaneous preadipocytes to mature adipocytes in culture. The medium is available in 100ml or 500ml bottles and is ready-to-use upon arrival.

Subcutaneous adipocyte medium

Adipocyte medium is used as a maintenance medium after differentiation and contains all of the factors necessary to support lipid accumulation in the maturing adipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous preadipocytes cryopreservation medium

Cryopreservation medium is used for long-term cryopreservation of subcutaneous preadipocytes. This medium has been specifically formulated for optimized recovery of human preadipocytes after thawing. It is available in 100ml bottles.

Order Information

Subcutaneous Media

Item#Item DescU/MPrice
BM-1Subcutaneous Basal Medium500 ml$85.00
PM-1Subcutaneous Preadipocyte Medium500 ml$93.00
DM-2Subcutaneous Preadipocyte Differentiation Medium100ml$100.00
DM-2-500Subcutaneous Preadipocyte Differentiation Medium500ml$429.00
AM-1Subcutaneous Adipocyte Maintenance Medium500ml$93.00
FM-1-100Preadipocyte Cryopreservation medium100ml$167.00
KT-02Subcutaneous Adipocyte Differentiation Kit: sufficient media to differentiate 1 96 well plate human subcutaneous preadipocytes to adipocytes. 50ml PM-1, 25ml DM-2, 25ml AM-1, 1 blank sterile 96 well plateKIT$77.00

If you require special media modifications, we offer a Custom Media Service starting at one bottle per order. Please contact us for more details or consult our Price List (.pdf). Examples of some common modifications include: Phenol-Red free, Serum-free, Dexamethasone-free, Insulin-free, PPAR Gamma Agonist-free and Antibiotic-free.

Recent Publications: View
Completely serum-free and chemically defined adipocyte development and maintenance
Ann-Cathrin Volz, Petra Juliane Kluger
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Histopathological and Behavioral Assessments of Aging Effects on Stem Cell Transplants in an Experimental Traumatic Brain Injury
Jea-Young Lee, Roger Lin, Hung Nguyen, M. Grant Liska, Trenton Lippert, Yuji Kaneko, Cesar V. Borlongan
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MicroRNA-221-3p regulates Angiopoietin-like 8 (ANGPTL8) expression in adipocytes Raghavendra Mysore Francisco J Ortega Jéssica Latorre Maria AhonenHanna Savolainen-Peltonen Pamela Fischer-Posovszky Martin WabitschVesa M Olkkonen José M Fernández-Real P A Nidhina Haridas
The Journal of Clinical Endocrinology & Metabolism, jc.2017-00453,
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Adipose tissue macrophages impair preadipocyte differentiation in humans Li Fen Liu , Colleen M. Craig, Lorna L. Tolentino, Okmi Choi, John Morton, Homero Rivas, Samuel W. Cushman, Edgar G. Engleman, Tracey McLaughlin
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EGF and hydrocortisone as critical factors for the co-culture of adipogenic differentiated ASCs and endothelial cells Ann-Cathrin Volza, Birgit Huberb, Alina Maria Schwandtc, Petra Juliane Kluger
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MALAT1 in human adipose stem cells modulates survival and alternative splicing of PKCδII in HT22 cells Ghattas El Bassit, Rekha S. Patel, Gay Carter, Vyshakh Shibu, Achintya Patel, Shijie Song, Michel Murr, Denise R. Cooper, Paula C. Bickford and Niketa A. Patel
DOI: 10.1210/en.2016-1819
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Eicosapentaenoic acid promotes mitochondrial biogenesis and beige-like features in subcutaneous adipocytes from overweight subjects L.M. Laiglesia, S. Lorente-Cebrián, P.L. Prieto-Hontoria1, M. Fernández-Galilea2, S.M.R. Ribeiro, N. Sáinz, J.A. Martínez, M.J. Moreno-Aliaga
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Serial changes in the proliferation and differentiation of adipose-derived stem cells after ionizing radiation Woonhyeok Jeong, Xiao Yang, Jeongmi Lee, Youngwook Ryoo, Jinhee Kim, Youngkee Oh, Sunyoung Kwon, Dalie Liu and Daegu Son
Stem Cell Research & Therapy20167:117
DOI: 10.1186/s13287-016-0378-0
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Screening for Identification of Personalized Food to Promote Adiponectin Secretion in Patients with Cancer MIKI SAKAUE, KAZUHISA MAEDA, SATOSHI OHNO and TOSHINORI ITO
Anticancer Research July 2016 vol. 36 no. 73673-3677
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Prostaglandin E 2 Exerts Multiple Regulatory Actions on Human Obese Adipose Tissue Remodeling, Inflammation, Adaptive Thermogenesis and Lipolysis Verónica García-Alonso, Esther Titos, Jose Alcaraz-Quiles, Bibiana Rius, Aritz Lopategi, Cristina López-Vicario, Per-Johan Jakobsson, Salvadora Delgado, Juanjo Lozano, Joan Clária
Published: April 28, 2016
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III. CHAPTER II: Ellagic acid inhibits adipocyte differentiation through coactivator associated arginine methyltransferase 1-mediated chromatin modification Inhae Kanga,b, Meshail Oklaa,b, and Soonkyu Chung
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Hypoxia alters the expression of dipeptidyl peptidase 4 (DPP4) and induces developmental remodeling of human preadipocytes Helena H. Chowdhury, Jelena Velebit, Nataŝa Radić, Vito Franĉiĉ , Marko Kreft, Robert Zorec
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Mechanisms of peroxisome proliferator activated receptor γ regulation by non-steroidal anti-inflammatory drugs Ana C. Puhl, Flora A. Milton, Aleksandra Cvoro, Douglas H. Sieglaff, Jéssica C.L. Campos, Amanda Bernardes, Carly S. Filgueira, Jan Lammel Lindemann, Tuo Deng, Francisco A.R. Neves, Igor Polikarpov, and Paul Webb
Nucl Recept Signal. 2015; 13: e004
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The Effects of Platelet-rich Plasma on Cell Proliferation and Adipogenic Potential of Adipose-derived Stem Cells Dr. Han-Tsung Liao, Mr. Isaac B James, Prof. Kacey Marra, and Dr. J. Peter Rubin. Tissue Engineering Part A. -Not available-, ahead of print. doi:10.1089/ten.TEA.2015.0159
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Expression analysis of human adipose-derived stem cells during in vitro differentiation to an adipocyte lineage Latha Satish, J. Michael Krill-Burger, Phillip H. Gallo, Shelley Des Etages, Fang Liu,Brian J. Philips, Sudheer Ravuri, Kacey G. Marra, William A. LaFramboise, Sandeep Kathju and J. Peter Rubin
BMC Medical Genomics 2015, 8:41 doi:10.1186/s12920-015-0119-8
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Decelerating mature adipocyte dedifferentiation by media composition
Mrs. Birgit Huber and Dr. Petra J Kluger. Tissue Engineering Part C: Methods. doi:10.1089/ten.TEC.2015.0166.
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Expression analysis of human adipose-derived stem cells during in vitro differentiation to an adipocyte lineage Latha Satish, J. Michael Krill-Burger, Phillip H. Gallo, Shelley Des Etages, Fang Liu, Brian J. Philips, Sudheer Ravuri, Kacey G. Marra, William A. LaFramboise, Sandeep Kathju and J. Peter Rubin
BMC Medical Genomics 2015, 8:41 doi:10.1186/s12920-015-0119-8
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In Vitro Effects of Bisphenol A β-D-Glucuronide (BPA-G) on Adipogenesis in Human and Murine Preadipocytes Jonathan G. Boucher, Adéle Boudreau, Shaimaa Ahmed, and Ella Atlas
Environmental Health Perspectives, May 2015
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MicroRNA-223 Expression Is Upregulated in Insulin Resistant Human Adipose Tissue Tung-Yueh Chuang, Hsiao-Li Wu, Chen-Chun Chen, Gloria Mabel Gamboa, Lawrence C. Layman, Michael P. Diamond, Ricardo Azziz, and Yen-Hao Chen
Journal of Diabetes Research Article ID 943659 (March 2015)
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Alteration of chromosome positioning during adipocyte differentiation Masahiko Kuroda, Hideyuki Tanabe, Keiichi Yoshida, Kosuke Oikawa, Akira Saito, Tomoharu Kiyuna, Hiroshi Mizusawa, and Kiyoshi Mukai J. Cell Sci., Nov 2004; 117: 5897 - 5903. download pdf
Conjugated Linoleic Acid Promotes Human Adipocyte Insulin Resistance through NFB-dependent Cytokine Production Soonkyu Chung, J. Mark Brown, J. Nathan Provo, Robin Hopkins, and Michael K. McIntosh J. Biol. Chem., Nov 2005; 280: 38445 - 38456. download pdf
FGF-21 as a novel metabolic regulator Alexei Kharitonenkov, Tatiyana L. Shiyanova, Anja Koester, Amy M. Ford, Radmila Micanovic, Elizabeth J. Galbreath, George E. Sandusky, Lisa J. Hammond, Julie S. Moyers, Rebecca A. Owens, Jesper Gromada, Joseph T. Brozinick, Eric D. Hawkins, Victor J. Wroblewski, De-Shan Li, Farrokh Mehrbod, S. Richard Jaskunas, and Armen B. Shanafelt J. Clin. Invest., Jun 2005; 115: 1627 - 1635. download pdf
Human adipose-derived stem cells: isolation, characterization and applications in surgery. Locke, M., Windsor, J. and Dunbar, P. R. (2009). ANZ Journal of Surgery, 79: 235-244. doi: 10.1111/j.1445-2197.2009.04852.x download pdf
Prediction of Naphthalene Bioaccumulation Using an Adipocyte Cell Line Model. Viravaidya, K. and Shuler, M. L. (2002). Biotechnology Progress, 18: 174-181. doi: 10.1021/bp0101684 download pdf
Differential estrogenic 17beta-hydroxysteroid dehydrogenase activity and type 12 17beta-hydroxysteroid dehydrogenase expression levels in preadipocytes and differentiated adipocytes V. Bellemare, P. Laberge, S. Noel, A. Tchernof and V. Luu-The J Steroid Biochem Mol Biol. 2009; 114 (3-5): 129-34. download pdf
Pathways of adipose tissue androgen metabolism in women: depot differences and modulation by adipogenesis K. Blouin, M. Nadeau, J. Mailloux, M. Daris, S. Lebel, V. Luu-The and A. Tchernof Am J Physiol Endocrinol Metab. 2009; 296 (2): E244-55. download pdf
Reduction of Neu5GC Xenoantigen on Human ADSC/MSCs lead to Them as Safer and More Useful Cell Sources for Realizing Various Stem Cell Therapies H. Komoda, H. Okura, C. M. Lee, N. Sougawa, T. Iwayama, T. Hashikawa, A. Saga, A. Yamamoto, A. Ichinose, S. Murakami, Y. Sawa and A. Matsuyama Tissue Eng Part A. 2009; Epub ahead of print download pdf
Identification and characterization of adult stem cells from human orbital adipose tissue B. S. Korn, D. O. Kikkawa and K. C. Hicok Ophthal Plast Reconstr Surg. 2009; 25 (1): 27-32. download pdf
On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels W. Lee, V. Lee, S. Polio, P. Keegan, J. H. Lee, K. Fischer, J. K. Park and S. S. Yoo Biotechnol Bioeng. 2009; download pdf
The Ron receptor tyrosine kinase negatively regulates mammary gland branching morphogenesis S. E. Meyer, G. M. Zinser, W. D. Stuart, P. Pathrose and S. E. Waltz Dev Biol. 2009; 333 (1): 173-85. download pdf
Properties of Hepatocyte-like Cell Clusters Derived from human adipose tissue-derived mesenchymal stem cells H. Okura, H. Komoda, A. Saga, A. Kakuta-Yamamoto, Y. Hamada, Y. Fumimoto, C. M. Lee, A. Ichinose, Y. Sawa and A. Matsuyama Tissue Eng Part C Methods. 2009; Epub ahead of print download pdf
Subcutaneous fat shows higher thyroid hormone receptor-alpha1 gene expression than omental fat F. J. Ortega, J. M. Moreno-Navarrete, V. Ribas, E. Esteve, J. I. Rodriguez-Hermosa, B. Ruiz, B. Peral, W. Ricart, A. Zorzano and J. M. Fernandez-Real Obesity (Silver Spring). 2009; 17 (12): 2134-41. download pdf
Tissue engineering of injectable soft tissue filler: using adipose stem cells and micronized acellular dermal matrix G. Yoo and J. S. Lim J Korean Med Sci. 2009; 24 (1): 104-9. download pdf
MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma C. W. Younce, A. Azfer and P. E. Kolattukudy J Biol Chem. 2009; 284 (40): 27620-8. download pdf
Progesterone metabolism in adipose cells Y. Zhang, M. Nadeau, F. Faucher, O. Lescelleur, S. Biron, M. Daris, C. Rheaume, V. Luu-The and A. Tchernof Mol Cell Endocrinol. 2009; 298 (1-2): 76-83. download pdf

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