Subcutaneous Preadipocyte and Adipocyte Media

All ZenBio media products are ready-to-use when purchased. No mixing of growth factors or antibiotics is necessary. All media products are available as phenol-red free, serum-free, or specifically customized to meet your research needs.

Subcutaneous basal medium

Basal medium is serum-free and contains essential additives to maintain subcutaneous cells for up to 3 days. It can be supplemented for specific research purposes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous preadipocyte medium

Preadipocyte medium is used to plate preadipocytes prior to differentiation and contains growth factors necessary for expansion of subcutaneous preadipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous differentiation medium

Differentiation medium is used to differentiate subcutaneous preadipocytes to mature adipocytes in culture. The medium is available in 100ml or 500ml bottles and is ready-to-use upon arrival.

Subcutaneous adipocyte medium

Adipocyte medium is used as a maintenance medium after differentiation and contains all of the factors necessary to support lipid accumulation in the maturing adipocytes. The medium is available in 500ml bottles and is ready-to-use upon arrival.

Subcutaneous preadipocytes cryopreservation medium

Cryopreservation medium is used for long-term cryopreservation of subcutaneous preadipocytes. This medium has been specifically formulated for optimized recovery of human preadipocytes after thawing. It is available in 100ml bottles.

Subcutaneous Media
ItemUnitCatalog #Price
Subcutaneous Basal Medium500 mlBM-1$61.00
Subcutaneous Preadipocyte Medium500 mlPM-1$67.00
Subcutaneous Differentiation Medium100 mlDM-2$72.00
Subcutaneous Differentiation Medium500 mlDM-2-500$333.00
Subcutaneous Adipocyte Medium500 mlAM-1$67.00
Cryopreservation medium100 mlFM-1-100$139.00

If you require special media modifications, we offer a Custom Media Service starting at one bottle per order. Please contact us for more details or consult our Price List. Examples of some common modifications include: Phenol-Red free, Serum-free, Dexamethasone-free, Insulin-free, PPAR Gamma Agonist-free and Antibiotic-free.

Recent Publications:

Differential estrogenic 17beta-hydroxysteroid dehydrogenase activity and type 12 17beta-hydroxysteroid dehydrogenase expression levels in preadipocytes and differentiated adipocytes
V. Bellemare, P. Laberge, S. Noel, A. Tchernof and V. Luu-The
J Steroid Biochem Mol Biol. 2009; 114 (3-5): 129-34.

Pathways of adipose tissue androgen metabolism in women: depot differences and modulation by adipogenesis
K. Blouin, M. Nadeau, J. Mailloux, M. Daris, S. Lebel, V. Luu-The and A. Tchernof
Am J Physiol Endocrinol Metab. 2009; 296 (2): E244-55.

Reduction of Neu5GC Xenoantigen on Human ADSC/MSCs lead to Them as Safer and More Useful Cell Sources for Realizing Various Stem Cell Therapies
H. Komoda, H. Okura, C. M. Lee, N. Sougawa, T. Iwayama, T. Hashikawa, A. Saga, A. Yamamoto, A. Ichinose, S. Murakami, Y. Sawa and A. Matsuyama
Tissue Eng Part A. 2009; Epub ahead of print

Identification and characterization of adult stem cells from human orbital adipose tissue
B. S. Korn, D. O. Kikkawa and K. C. Hicok
Ophthal Plast Reconstr Surg. 2009; 25 (1): 27-32.

On-demand three-dimensional freeform fabrication of multi-layered hydrogel scaffold with fluidic channels
W. Lee, V. Lee, S. Polio, P. Keegan, J. H. Lee, K. Fischer, J. K. Park and S. S. Yoo
Biotechnol Bioeng. 2009;

The Ron receptor tyrosine kinase negatively regulates mammary gland branching morphogenesis
S. E. Meyer, G. M. Zinser, W. D. Stuart, P. Pathrose and S. E. Waltz
Dev Biol. 2009; 333 (1): 173-85.

Properties of Hepatocyte-like Cell Clusters Derived from human adipose tissue-derived mesenchymal stem cells
H. Okura, H. Komoda, A. Saga, A. Kakuta-Yamamoto, Y. Hamada, Y. Fumimoto, C. M. Lee, A. Ichinose, Y. Sawa and A. Matsuyama
Tissue Eng Part C Methods

. 2009; Epub ahead of print

Subcutaneous fat shows higher thyroid hormone receptor-alpha1 gene expression than omental fat
F. J. Ortega, J. M. Moreno-Navarrete, V. Ribas, E. Esteve, J. I. Rodriguez-Hermosa, B. Ruiz, B. Peral, W. Ricart, A. Zorzano and J. M. Fernandez-Real
Obesity (Silver Spring). 2009; 17 (12): 2134-41.

Tissue engineering of injectable soft tissue filler: using adipose stem cells and micronized acellular dermal matrix
G. Yoo and J. S. Lim
J Korean Med Sci. 2009; 24 (1): 104-9.

MCP-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3T3-L1 pre-adipocytes without peroxisome proliferator-activated receptor gamma
C. W. Younce, A. Azfer and P. E. Kolattukudy
J Biol Chem. 2009; 284 (40): 27620-8.

Progesterone metabolism in adipose cells
Y. Zhang, M. Nadeau, F. Faucher, O. Lescelleur, S. Biron, M. Daris, C. Rheaume, V. Luu-The and A. Tchernof
Mol Cell Endocrinol. 2009; 298 (1-2): 76-83.

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