Support: Reagent Manuals
- 3T3-L1 Cryopreservation Medium (pdf)
- FM-1-100 Medium manuals (pdf)
- Dispase II (pdf)
Dispase II, a non-mammalian, partially purified, neutral protease isolated from culture filtrates of Bacillus polymyxa, has proven to be a rapid, effective, but gentle agent for tissue dissociation. This product is animal origin free. The mild pr oteolytic actions make this enzyme suitable for separating intact epidermis from the dermis and in tact epithelial sheets in culture from the substratum. Dispase cleaves fibronectin and type IV collagen, but not laminin, type V collagen, serum albumin, or transferrin. This protease is also used as a secondary enzyme in cell dissociation and tissue dissociation applications, commonly used with collagenase. Inhibitors include 1mM EDTA, EGTA, 1-10-phenanthroline, and heavy metals. This product is stable for 12 months upon arrival when stored at 2-8 °C.
- EZ Strip Cell (pdf)
EZ-Strip is a non-enzymatic, animal component free cell culture dissociation solution. When EZ-Strip is used in conjunction with either serum-containing or serum-free media, it gently dislodges adherent cells and eliminates the damage normally seen with trypsin based solutions, even if exposed to EZ-Strip for extended periods of time. This solution substitutes trypsin and is composed of a proprietary mixture of chelators in a balanced salt solution and shows excellent results when compared with enzymatic dissociation solutions.
- EZ-TITER (pdf)
Cell proliferation and viability measurements are important tools for research. The EZ-Titer assay is a fluorometric method for estimating the number of viable cells present in multiwell plates. It uses the indicator dye resazurin to measure metabolic capacity of cells - an indicator of viability. Cells that are metabolizing maintain a reducing environment within their cytosol. Non-viable cells rapidly lose metabolic activity and do not reduce the resazurin, and subsequently do not generate a fluorescent signal.