ZenBio now offers Cryopreserved and Cultured 3T3-L1 Preadipocytes!

3T3-L1 murine adipocytes have been fundamental in metabolic disease research for over 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular mechanisms associated with diabetes, obesity and related disorders.

We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using ZenBio's line of 3T3-L1 media.

3T3-LI murine adipocytes
"We have just conducted an experiment with the 3T3L1 cells that we obtained from ZenBio, and we are astounded by the results! In our previous experience with 3T3L1 cells obtained from ATCC and Sigma-Aldrich, it has always been a challenge to get a high percentage of the cells to differentiate into mature adipocytes...we obtained strong differentiation of virtually 100% of the cells to adipocytes on the first try!"
Vala Sciences, Inc

Cryopreserved & Cultured 3T3-L1 Preadipocytes

Item#Item DescU/MPrice
SP-L1-F3T3-L1 Preadipocytes Cryopreserved, (500,000 cells/vial)Vial$320.00
SP-L1-963T3-L1 Preadipocytes 96-well PlateEach$346.00
SP-L1-243T3-L1 Preadipocytes 24-well PlatePlate$346.00
SP-L1-483T3-L1 Preadipocytes 48-well PlateEach$346.00
SP-L1-T253T3-L1 Cultured Preadipocytes, T-25cm2 FlaskFlask$492.00


Item#Item DescU/MPrice
AM-1-L13T3-L1 Adipocyte Medium500 ml$96.00
AM-1-L1-PRF3T3-L1 Adipocyte Medium Phenol Red Free500 ml$110.00
AM-1-L1-IF3T3-L1 Adipocyte Medium WITHOUT Insulin500 ml$107.00
AM-1-L1-DF3T3-L1 Adipocyte Medium WITHOUT Dexamethasone500 ml$110.00
AM-1-L1-SF3T3-L1 Adipocyte Medium WITHOUT Serum500ml$110.00
AM-1-L1-ABF3T3-L1 Adipocyte Medium WITHOUT ANTIBIOTICS500ml$103.00
DM-2-L13T3-L1 Differentiation Medium100 ml$103.00
DM-2-L1-5003T3-L1 Differentiation Medium500ml$442.00
DM-2-L1-500-PRF3T3-L1 Differentiation Medium, WITHOUT Phenol Red500 ml$442.00
DM-2-L1-ABF3T3-L1 Differentiation Medium, WITHOUT ANTIBIOTICS500ml$442.00
DM-2-L1-PPG3T3-L1 Differentiation Medium WITHOUT PPAR gamma agonist100 ml$110.00
PM-1-L13T3-L1 Preadipocyte Medium500 ml$96.00
PM-1-L1-PRF3T3-L1 Preadipocyte Medium Phenol Red Free500 ml$110.00
PM-1-L1-ABF3T3-L1 Preadipocyte Medium, WITHOUT ANTIBIOTICS500ml$110.00
PM-1-L1-SF3T3-L1 Preadipocyte Medium, WITHOUT SERUM500ml$88.00
BM-1-L13T3-L1 Basal Medium500 ml$88.00
FM-1-L1Cryopreservation Medium for 3T3-L1 cells100 ml$172.00


Item#Item DescU/MPrice
LIP-1-L1Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS+ CELLS)Kit$688.00
LIP-1-L1-FLipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, WITH CRYOPRESERVED CELLS(REAGENTS+ CELLS)Kit$633.00
LIP-1-NCL1Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS ONLY)Kit$359.00
LIP-1-NCL1DIFLipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection, WITH 3T3-L1 Media to differentiate 1 96 well plate (REAGENTS+ MEDIA)Kit$473.00
KT-013T3-L1 Preadiopcyte Differentiation Kit Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 CellsKit$118.00
KT-01-PRF3T3-L1 Preadipocyte Differentiation Kit: Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 Cells (PHENOL RED- FREE)Kit$130.00
LIP-2-NC-L13T3-L1 Lipolysis Assay Kit, Free Fatty Acid+ Detection,100 point assay kit (REAGENTS ONLY)Kit$458.00
LIP-2-L13T3-L1 Lipolysis Assay Kit, Free Fatty Acid Detection (REAGENTS+ CELLS)Kit$787.00
LIP-3-NC-L13T3-L1 Lipolysis Assay KIT (REAGENTS ONLY) Combo-Free Fatty acid and glycerol releaseKit$648.00
LIP-3-L13T3-L1 Lipolysis Kit- Dual Detection Free Fatty Acid and Glycerol Release 100 point assay kit,+ (REAGENTS CELLS)Kit$918.00

All ZenBio media are available as without phenol-red (-PRF) and/or without serum (-SF). Please inquire for custom media formulations.

Figure 1 Lipid accumulation in 3T3-L1

Fig. 1. Lipid accumulation in 3T3-L1 cells cultured in ZenBio media.

3T3-L1 preadipocytes were seeded in 24 well plates and induced to differentiate 2 days post confluent using Zen Bio's DM-2-L1 for 3 days. Cells were then fed Zen Bio's AM-1-L1, with fresh media being added every other day. Phase contrast images were taken on day 7 (Panel A) and day 14 (Panel B) of differentiation using an Olympus IX60 microscope equipped with a STOP digital camera (20X magnification)

Contact Us For More Information

3T3-L1 Adipocyte Care Manual (.PDF)

A comprehensive list of products and prices can be found at Retail Prices (.PDF).

3T3-L1 Publications

Antioxidant treatment induces reductive stress associated with mitochondrial dysfunction in adipocytes

Eduard Peris, Peter Micallef, Alexandra Paul, Vilborg Palsdottir, Annika Enejder, Marco Bauzá-Thorbrügge, Charlotta S. Olofsson and Ingrid Wernstedt Asterholm

Insulin resistance uncoupled from dyslipidemia due to C-terminal PIK3R1 mutations

Isabel Huang-Doran, Patsy Tomlinson, Felicity Payne, Alexandra Gast, Alison Sleigh, William Bottomley,Julie Harris, Allan Daly, Nuno Rocha, Simon Rudge, Jonathan Clark, Albert Kwok, Stefano Romeo, Emma McCann, Barbara Müksch, Mehul Dattani, Stefano Zucchini, Michael Wakelam, Lazaros C. Foukas, David B. Savage, Rinki Murphy, Stephen O'Rahilly, Inês Barroso, and Robert K. Semple
JCI Insight. 2016 Oct 20; 1(17): e88766.

Click here for additional publications