3T3L1
Zen-Bio now offers cultured 3T3-L1 preadipocytes!We now offer plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using Zen-Bio's line of 3T3-L1 media.
| Cultured 3T3-L1 Preadipocytes | ||
|---|---|---|
| Item | Unit | Cat# |
| 3T3-L1 preadipocytes 96-well plate | Plate | SP-L1-96 |
| 3T3-L1 Adipocyte Kit (media only) | Kit | KT-01 |
| Reagents | ||
| 3T3-L1 Preadipocyte Medium | 500ml | PM-1-L1 |
| 3T3-L1 Differentiation Medium | 100ml | DM-2-L1 |
| 3T3-L1 Differentiation Medium | 500ml | DM-2-L1-500 |
| 3T3-L1 Adipocyte Medium | 500ml | AM-1-L1 |
| 3T3-L1 Basal Medium | 500ml | BM-1-L1 |
| Lipolysis Assay Kit w/ 96 well plate 3T3-L1 cells | Kit | LIP-1-L1 |
| Lipolysis Assay Kit (Reagents Only) | Kit | LIP-1-NCL1 |
All Zen-Bio media are available as without phenol-red (-PRF) and/or without serum (-SF). Please inquire for custom media formulations.
Fig. 1. Lipid accumulation in 3T3- L1 cells cultured in Zen Bio media.
3T3-L1 preadipocytes were seeded in 24 well plates and induced to differentiate 2 days post confluent using Zen Bio's DM-2-L1 for 3 days. Cells were then fed Zen Bio's AM-1-L1, with fresh media being added every other day. Phase contrast images were taken on day 7 (Panel A) and day 14 (Panel B) of differentiation using an Olympus IX60 microscope equipped with a STOP digital camera (20X magnification)
