ZenBio now offers Cryopreserved and Cultured 3T3-L1 Preadipocytes!

3T3-L1 murine adipocytes have been fundamental in metabolic disease research for over 30 years. Originally derived from Swiss mouse embryo tissue by Dr. Howard Green of Harvard Medical School, the 3T3-L1 system has been pivotal in advancing the understanding of basic cellular mechanisms associated with diabetes, obesity and related disorders.

We now offer both Cryopreserved and Plated sub-confluent 3T3-L1 preadipocytes in 96 well format and media for proliferation, differentiation, maintenance of 3T3-L1 preadipocytes to adipocytes. We also offer media and reagent kits validated using 3T3-L1 and/or primary rodent preadipocytes and adipocytes. High quality control tested 3T3-L1 preadipocytes designed to work consistently using ZenBio's line of 3T3-L1 media.

3T3-LI murine adipocytes
"We have just conducted an experiment with the 3T3L1 cells that we obtained from ZenBio, and we are astounded by the results! In our previous experience with 3T3L1 cells obtained from ATCC and Sigma-Aldrich, it has always been a challenge to get a high percentage of the cells to differentiate into mature adipocytes...we obtained strong differentiation of virtually 100% of the cells to adipocytes on the first try!"
Vala Sciences, Inc

Cryopreserved & Cultured 3T3-L1 Preadipocytes

Item#Item DescU/MPrice
SP-L1-F3T3-L1 Preadipocytes Cryopreserved, (500,000 cells/vial)Vial$320.00
SP-L1-963T3-L1 Preadipocytes 96-well PlateEach$346.00
SP-L1-243T3-L1 Preadipocytes 24-well PlatePlate$346.00
SP-L1-483T3-L1 Preadipocytes 48-well PlateEach$346.00
SP-L1-T253T3-L1 Cultured Preadipocytes, T-25cm2 FlaskFlask$492.00


Item#Item DescU/MPrice
AM-1-L13T3-L1 Adipocyte Medium500 ml$93.00
AM-1-L1-PRF3T3-L1 Adipocyte Medium Phenol Red Free500 ml$107.00
AM-1-L1-IF3T3-L1 Adipocyte Medium WITHOUT Insulin500 ml$107.00
AM-1-L1-DF3T3-L1 Adipocyte Medium WITHOUT Dexamethasone500 ml$107.00
AM-1-L1-SF3T3-L1 Adipocyte Medium WITHOUT Serum500ml$107.00
AM-1-L1-ABF3T3-L1 Adipocyte Medium WITHOUT ANTIBIOTICS500ml$100.00
DM-2-L13T3-L1 Differentiation Medium100 ml$100.00
DM-2-L1-5003T3-L1 Differentiation Medium500ml$429.00
DM-2-L1-500-PRF3T3-L1 Differentiation Medium, WITHOUT Phenol Red500 ml$429.00
DM-2-L1-ABF3T3-L1 Differentiation Medium, WITHOUT ANTIBIOTICS500ml$429.00
DM-2-L1-PPG3T3-L1 Differentiation Medium WITHOUT PPAR gamma agonist100 ml$107.00
PM-1-L13T3-L1 Preadipocyte Medium500 ml$93.00
PM-1-L1-PRF3T3-L1 Preadipocyte Medium Phenol Red Free500 ml$107.00
PM-1-L1-ABF3T3-L1 Preadipocyte Medium, WITHOUT ANTIBIOTICS500ml$107.00
PM-1-L1-SF3T3-L1 Preadipocyte Medium, WITHOUT SERUM500ml$85.00
BM-1-L13T3-L1 Basal Medium500 ml$85.00
FM-1-L1Cryopreservation Medium for 3T3-L1 cells100 ml$167.00


Item#Item DescU/MPrice
LIP-1-L1Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS+ CELLS)Kit$688.00
LIP-1-L1-FLipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, WITH CRYOPRESERVED CELLS(REAGENTS+ CELLS)Kit$633.00
LIP-1-NCL1Lipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection-100 point, (REAGENTS ONLY)Kit$359.00
LIP-1-NCL1DIFLipolysis Assay Kit for 3T3-L1 cells, Glycerol Detection, WITH 3T3-L1 Media to differentiate 1 96 well plate (REAGENTS+ MEDIA)Kit$473.00
KT-013T3-L1 Preadiopcyte Differentiation Kit Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 CellsKit$118.00
KT-01-PRF3T3-L1 Preadipocyte Differentiation Kit: Reagents sufficient to differentiate and maintain 1x96 well plate of 3T3-L1 Cells (PHENOL RED- FREE)Kit$130.00
LIP-2-NC-L13T3-L1 Lipolysis Assay Kit, Free Fatty Acid+ Detection,100 point assay kit (REAGENTS ONLY)Kit$458.00
LIP-2-L13T3-L1 Lipolysis Assay Kit, Free Fatty Acid Detection (REAGENTS+ CELLS)Kit$787.00
LIP-3-NC-L13T3-L1 Lipolysis Assay KIT (REAGENTS ONLY) Combo-Free Fatty acid and glycerol releaseKit$648.00
LIP-3-L13T3-L1 Lipolysis Kit- Dual Detection Free Fatty Acid and Glycerol Release 100 point assay kit,+ (REAGENTS CELLS)Kit$918.00

All ZenBio media are available as without phenol-red (-PRF) and/or without serum (-SF). Please inquire for custom media formulations.

Figure 1 Lipid accumulation in 3T3-L1

Fig. 1. Lipid accumulation in 3T3-L1 cells cultured in ZenBio media.

3T3-L1 preadipocytes were seeded in 24 well plates and induced to differentiate 2 days post confluent using Zen Bio's DM-2-L1 for 3 days. Cells were then fed Zen Bio's AM-1-L1, with fresh media being added every other day. Phase contrast images were taken on day 7 (Panel A) and day 14 (Panel B) of differentiation using an Olympus IX60 microscope equipped with a STOP digital camera (20X magnification)

Contact Us For More Information

3T3-L1 Adipocyte Care Manual (.PDF)

A comprehensive list of products and prices can be found at Retail Prices (.PDF).

Recent Publications:View
Evaluation of anti-insulin receptor antibodies as potential novel therapies for human insulin receptoropathy using cell culture models Gemma V. Brierley & Kenneth Siddle & Robert K. Semple
DOI https://doi.org/10.1007/s00125-018-4606-2
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Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action Caslin A. Gilroy, Stefan Roberts, Ashutosh Chilkoti
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Transcriptional Regulation of TCF/LEF and PPAR γ by Daidzein and Genistein in 3T3-L1 Preadipocytes Jeremy E. Davis and Darcie Hastings
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Xinxia Wang, Baofa Sun, Qin Jiang, Ruifan Wu, Min Cai, Yongxi Yao, Qing Liu, Hailing Shi, Jie Feng & Yizhen Wang International Journal of Obesity (2018), doi:10.1038/s41366-018-0027-z
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In vitro anti-diabetic effect of flavonoids and pheophytins from Allophylus cominia Sw. on the glucose uptake assays by HepG2, L6, 3T3-L1 and fat accumulation in 3T3 ... D.G. Semaana, J.O. Igolia, c, L. Younga, A.I. Graya, E.G. Rowana, E. Marrerob
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The high-production volume fungicide pyraclostrobin induces triglyceride accumulation associated with mitochondrial dysfunction, and promotes adipocyte ... Anthony L. Luz, Christopher D. Kassotis, Heather M. Stapleton, Joel N. Meyer
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Nonionic Ethoxylated Surfactants Induce Adipogenesis in 3T3-L1 Cells Christopher D Kassotis Erin M Kollitz P Lee Ferguson Heather M Stapleton
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Interleukin-15 derived from Guanylin-GC-C-expressing macrophages inhibits fatty acid synthase in adipocytes Sayaka Akieda-Asaia, Takanori Idaa, Mikiya Miyazatob, Kenji Kangawab, Yukari Datea https://doi.org/10.1016/j.peptides.2017.10.012
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Mesoderm specific transcript localization in the ER and ER-lipid droplet interface supports a role in adipocyte hypertrophy Igor Prudovsky, Rea P. Anunciado-Koza, Chester G. Jacobs, Doreen Kacer, Matthew E. Siviski, Robert A. Koza
DOI: 10.1002/jcb.26429
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Retinol saturase modulates lipid metabolism and the production of reactive oxygen species Xiao-YanPang, SuyaWang, Michael J.Jurczak, Gerald I.Shulman, Alexander R.Moise
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Dibutyltin Compounds Effects on PPARγ/RXRα Activity, Adipogenesis, and Inflammation in Mammalians Cells Flora A. Milton, Mariella G. Lacerda, Simone B. P. Sinoti, Pedro G. Mesquita, Dileesh Prakasan, Michella S. Coelho, Caroline L. de Lima, Alexandre G. Martini, Gabriela T. Pazzine, Maria de F. Borin, Angelica A. Amato and Francisco de A. R. Neves
Front. Pharmacol., 02 August 2017 https://doi.org/10.3389/fphar.2017.00507
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Ciliary Hedgehog Signaling Restricts Injury-Induced Adipogenesis Daniel Kopinke, Elle C. Roberson, Jeremy F. Reiter
DOI: http://dx.doi.org/10.1016/j.cell.2017.06.035
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Characterization of Adipogenic Activity of House Dust Extracts and Semi-Volatile Indoor Contaminants in 3T3-L1 Cells Christopher D. Kassotis, Kate Hoffman, and Heather M. Stapleton
Environ. Sci. Technol., Article ASAP
DOI: 10.1021/acs.est.7b01788
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Total control of fat cells from adipogenesis to apoptosis using a xanthene analog Ching-Hsuan Tung, Myung Shin Han , Jianjun Qi
PLoS ONE 12(6): e0179158. https://doi.org/10.1371/journal. pone.0179158
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Phosphorylation of TXNIP by AKT Mediates Acute Influx of Glucose in Response to Insulin Althea N. Waldhart, Holly Dykstra, Anderson S. Peck, Elissa A. Boguslawski, Zachary B. Madaj, Jennifer Wen, Kelsey Veldkamp, Matthew Hollowell, Bin Zheng, Lewis C. Cantley, Timothy E. McGraw, Ning Wu
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Leucine-nicotinic acid synergy stimulates AMPK/Sirt1 signaling and regulates lipid metabolism and lifespan in Caenorhabditis elegans, and hyperlipidemia and ... Antje Bruckbauer, Jheelam Banerjee, Quiang Cao, Xin Cui, Jia Jing, Lin Zha, Fenfen Li, Bingzhong Xue, Hang Shi, and Michael B Zemel
Am J Cardiovasc Dis. 2017; 7(2): 33-47.
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A biomimetic hydrogel functionalized with adipose ECM components as a microenvironment for the 3D culture of human and murine adipocytes Fiona Louis, Pauline Pannetier, Zied Souguir, Didier Le Cerf, Philippe Valet, Jean-Pierre Vannier, Guillaume Vidal, Elise Demange
DOI: 10.1002/bit.26306
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Novel role for Wnt inhibitor APCDD1 in adipocyte differentiation: implications for diet-induced obesity Nicole K.H. Yiew, Tapan K. Chatterjee, Yao Liang Tang, Rod Pellenberg, Brian K. Stansfield, Zsolt Bagi, David J. Fulton, David W. Stepp, Weiqin Chen, Vijay Patel, Vinayak M. Kamath, Sheldon E. Litwin, David Y Hui, Steven M. Rudich, Ha Won Kim and Neal L. Weintraub
doi: 10.1074/jbc.M116.758078
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IL-17 axis accelerates the inflammatory progression of obese in mice via TBK1 and IKBKE pathway Seung Hoon Leea, JooYeon Jhuna, Jae-Kyung Byuna, Eun-Kyung Kima, KyoungAh Jungb, Ji Eun Leec, Jong Young Choid, Sung-Hwan Parka, Mi-La Choa,
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Parkin‐mediated mitophagy is downregulated in browning of white adipose tissue David Taylor, Roberta A. Gottlieb
DOI: 10.1002/oby.21786
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FAK signalling controls insulin sensitivity through regulation of adipocyte survival Cynthia T. Luk, Sally Yu Shi, Erica P. Cai, Tharini Sivasubramaniyam, Mansa Krishnamurthy, Jara J. Brunt, Stephanie A. Schroer, Daniel A. Winer & Minna Woo
Nat. Commun. 8,14360 doi: 10.1038/ncomms14360 (2017).
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Characterization of Adipogenic Chemicals in Three Different Cell Culture Systems: Implications for Reproducibility Based on Cell Source and Handling Christopher D. Kassotis, Lauren Masse, Stephanie Kim, Jennifer J. Schlezinger, Thomas F. Webster & Heather M. Stapleton
Scientific Reports 7, Article number: 42104 (2017)
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Abscisic acid enhances glucose disposal and induces brown fat activity in adipocytes in vitro and in vivo Laura Sturlaa, Elena Manninoa, Sonia Scarfìb, Santina Bruzzonea, Mirko Magnonea, Giovanna Socialia, Valeria Booza, Lucrezia Guidaa, Tiziana Vigliaroloa, Chiara Fresiaa, Laura Emionitec, Ambra Buschiazzod, Cecilia Marinie, f, Gianmario Sambucetid, f, Antonio De Floraa, Elena Zocchi
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Insulin resistance uncoupled from dyslipidemia due to C-terminal PIK3R1 mutations Isabel Huang-Doran, Patsy Tomlinson, Felicity Payne, Alexandra Gast, Alison Sleigh, William Bottomley,Julie Harris, Allan Daly, Nuno Rocha, Simon Rudge, Jonathan Clark, Albert Kwok, Stefano Romeo, Emma McCann, Barbara Müksch, Mehul Dattani, Stefano Zucchini, Michael Wakelam, Lazaros C. Foukas, David B. Savage, Rinki Murphy, Stephen O'Rahilly, Inês Barroso, and Robert K. Semple
JCI Insight. 2016 Oct 20; 1(17): e88766.
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Soshiho-tang aqueous extract exerts antiobesity effects in high fat diet-fed mice and inhibits adipogenesis in 3T3-L1 adipocytes Sae-Rom Yoo, Mee-young Lee, Byoung-Kab Kang, Hyeun-Kyoo Shin, Soo-Jin Jeong
Korea Institute of Oriental Medicine
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White Adipocyte Adiponectin Exocytosis is Stimulated Via β3 Adrenergic Signaling and Activation of Epac1: Catecholamine Resistance in Obesity and Type 2... Ali M. Komai, Saliha Musovic, Eduard Peris, Ahmed Alrifaiy, Mickaël F. El Hachmane, Marcus Johansson,Ingrid Wernstedt Asterholm, Charlotta S. Olofsson
Diabetes 2016 Aug; db151597.http://dx.doi.org/10.2337/db15-1597
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Adipogenesis, lipogenesis and lipolysis is stimulated by mild but not severe hypoxia in 3T3-L1 cells Martin Weiszensteina, Martina Musutovaa, Andrea Plihalovaa, Katerina Westlakea, c, Moustafa Elkalafb, Michal Kocd, Antonin Prochazkae, Jan Palad, Sumeet Gulatia, Jan Trnkab, Jan Polak
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HMGB1 is secreted by 3T3-L1 adipocytes through JNK signaling and the secretion is partially inhibited by adiponectin Toshiaki Shimizu, Munekazu Yamakuchi, Kamal Krishna Biswas, Bibek Aryal, Shingo Yamada, Teruto Hashiguchi and Ikuro Maruyama
DOI: 10.1002/oby.21549
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Antidiabetic Potential of Purple and Red Rice (Oryza sativa L.) Bran Extracts Stephen M. Boue, Kim W. Daigle, Ming-Hsuan Chen, Heping Cao, and Mark L. Heiman
J. Agric. Food Chem., DOI: 10.1021/acs.jafc.6b01909
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Ginger oil-mediated down-regulation of adipocyte specific genes inhibits adipogenesis and induces apoptosis in 3T3-L1 adipocytes Thamilvaani Manaharan and M. S. Kanthimathi
Biochemistry and Biotechnology Research Vol. 4(2), pp. 38-47, May 2016 ISSN: 2354-2136
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The commonly used nonionic surfactant Span 80 has RXRα transactivation activity, which likely increases the obesogenic potential of oil dispersants and food ... Robert R. Bowersa, Alexis M. Temkinb, Louis J. Guilletteb, c, John E. Baatzc, d, Demetri D. Spyropoulos
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The Effect of Pericellular Oxygen Levels on Proteomic Profile and Lipogenesis in 3T3-L1 Differentiated Preadipocytes Cultured on Gas-Permeable Cultureware Martin Weiszenstein, Nela Pavlikova, Moustafa Elkalaf, Petr Halada, Ondrej Seda, Jan Trnka, Jan Kovar, Jan Polak
Published: March 29, 2016 http://dx.doi.org/10.1371/journal.pone.0152382
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Ahnak stimulates BMP2‐mediated adipocyte differentiation through Smad1 activation Sunmee Shin, Je Kyung Seong, and Yun Soo Bae
DOI: 10.1002/oby.21367
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Curcumin analogues as selective fluorescence imaging probes for brown adipose tissue and monitoring browning Xueli Zhang, Yanli Tian, Hongbin Zhang, Amol Kavishwar, Matthew Lynes, Anna-Liisa Brownell, Hongbin Sun, Yu-Hua Tseng, Anna Moore & Chongzhao Ran
Scientific Reports 5, Article number: 13116 (2015), doi:10.1038/srep13116
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Role of Micro RNA-205 in Promoting Visceral Adiposity of NZ10 Mice with Polygenic Susceptibility for Type 2 Diabetes Nikhil Adi1,Jennipher Adi1,Liliana Cesar, Paul Kurlansky, Arthur Agatston and Keith A Webster
Adi et al., J Diabetes Metab 2015, 6:7 http://dx.doi.org/10.4172/2155-6156.1000574
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Traditional Korean herbal formula Samsoeum attenuates adipogenesis by regulating the phosphorylation of ERK1/2 in 3T3-L1 cells Soo-Jin Jeong,1 Sae-Rom Yoo,2 Chang-Seob Seo, 2 Hyeun-Kyoo Shin
KM Convergence Research Division, 2 K-herb Research Center, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea
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Hydroxytyrosol Inhibits Cannabinoid CB1 Receptor Gene Expression in 3T3-L1 Preadipocyte Cell Line Valeria Tutino, Antonella Orlando, Francesco Russo and Maria Notarnicola
Journal of Cellular Physiology, DOI: 10.1002/jcp.25094
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PPARγ partial agonist GQ-16 strongly represses a subset of genes in 3T3-L1 adipocytes Flora Aparecida Miltona, b, Aleksandra Cvorob, Angelica A. Amatoa, Douglas H. Sieglaffb, Carly S. Filgueirab, Anithachristy Sigamani Arumanayagamb, Maria do Carmo Alves de Limac, Ivan Rocha Pittac, Francisco de Assis Rocha Nevesa, Paul Webb
Biochemical and Biophysical Research Communications
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Traditional medicine yanggyuksanhwa-tang inhibits adipogenesis and suppresses proliferator-activated receptor gamma expression in 3T3-L1 cells Soo-Jin Jeong, Sae-Rom Yoo, Chang-Seob Seo, Hyeun-Kyoo Shin
DOI: 10.4103/0973-1296.160456, Pharmacognosy Magazine
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Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPARγ Activity in Vitro and in Vivo: Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen Alexis M. Temkin, Robert R. Bowers, Margaret E. Magaletta, Steven Holshouser, Adriana Maggi, Paolo Ciana, Louis J. Guillette, John A. Bowden, John R. Kucklick, John E. Baatz, and Demetri D. Spyropoulos http://dx.doi.org/10.1289/ehp.1409672, Advance Publication: 2 July 2015
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Nuclear-localized CTP: phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis Adam J. Aitchison, Daniel J. Arsenault, and Neale D. Ridgway
Mol. Biol. Cell June 24, 2015 mbc.E15-03-0159
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Translocator protein (18kDa) as a pharmacological target in adipocytes to regulate glucose homeostasis
J Li, V Papadopoulos - Biochemical Pharmacology, 2015
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Adipocyte Secreted Factors Enhance Aggressiveness of Prostate Carcinoma Cells Ângela Moreira, Sofia S. Pereira, Madalena Costa, Tiago Morais, Ana Pinto, Rúben Fernandes, Mariana P. Monteiro
Published: April 30, 2015DOI: 10.1371/journal.pone.0123217
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Leucine Amplifies the Effects of Metformin on Insulin Sensitivity and Glycemic Control in Diet-Induced Obese Mice Lizhi Fu, Antje Bruckbauer, Fenfen Li, Qiang Cao, Xin Cui, Rui Wu, Michael B. Zemel Hang Shia, Bingzhong Xue
Metabolism Clinical and Experimental, March 2015
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EPAS1 Promotes Adipose Differentiation in 3T3-L1 Cells. Shigeki Shimba, Taira Wada, Shuntaro Hara, and Masakatsu Tezuka J. Biol. Chem., Sep 2004; 279: 40946 - 40953 download pdf
Expression, regulation, and triglyceride hydrolase activity of Adiponutrin family members. Andrew C. Lake, Ying Sun, Jian-Liang Li, Jae Eun Kim, Jeremy W. Johnson, Dongmei Li, Tracy Revett, Heather H. Shih, Wei Liu, Janet E. Paulsen, and Ruth E. Gimeno J. Lipid Res., Nov 2005; 46: 2477 - 2487. download pdf
Isoginkgetin enhances adiponectin secretion from differentiated adiposarcoma cells via a novel pathway involving AMP-activated protein kinase. Guohong Liu, Mirta Grifman, James Macdonald, Peter Moller, Flossie Wong-Staal, and Qi-Xiang Li J. Endocrinol., Sep 2007; 194: 569 - 578. download pdf
Comparison of messenger RNA distribution for 60 proteins in fat cells vs the nonfat cells of human omental adipose tissue. J. N. Fain, B. Buehrer, S. W. Bahouth, D. S. Tichansky and A. K. Madan Metabolism. 2008; 57 (7): 1005-15. download pdf
Resveratrol Potentiates Genistein's Antiadipogenic and Proapoptotic Effects in 3T3-L1 Adipocytes. Srujana Rayalam, Mary Anne Della-Fera, Jeong-Yeh Yang, Hea Jin Park, Suresh Ambati, and Clifton A. Baile J. Nutr., Dec 2007; 137: 2668 - 2673. download pdf
Essential role of mannose-binding lectin-associated serine protease-1 in activation of the complement factor D
M. Takahashi, Y. Ishida, D. Iwaki, K. Kanno, T. Suzuki, Y. Endo, Y. Homma and T. Fujita
J Exp Med. 2010; 207 (1): 29-37, S1-3.
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Rosiglitazone abrogates bleomycin-induced scleroderma and blocks profibrotic responses through peroxisome proliferator-activated receptor-gamma
M. Wu, D. S. Melichian, E. Chang, M. Warner-Blankenship, A. K. Ghosh and J. Varga
Am J Pathol. 2009; 174 (2): 519-33.
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