Adipocyte NF-κB Activation
Inflammatory activity in fat tissue is related to insulin resistance and obesity-related metabolic disease. Nuclear factor-kappaB (NF-κB) is a regulator of many genes involved in the inflammatory response. Inhibitors of NF-κB activation may be effective in preventing obesity-related diseases. We format our assay in 96-well plates using adipocytes from different fat depots and can identify NF-κB activators or inhibitors.
Figure 1 shows the activation of NF-κB by increasing TNFα concentrations via increased p65 phosphorylation.
Figure 2 shows the inhibition of TNFα stimulated NF-κB by BAY 11-7085. p65 phosphorylation is inhibited by increasing concentrations of BAY 11-7085.
Ordering Information
| Assay | Cat# | Price |
|---|---|---|
| Adipocyte NF-κB Activation | CA-27 | Call |
