ZenComplete™: Glucose Uptake

Diabetes is a prevalent metabolic disease affecting more than 25 million Americans. About 90% to 95% of patients have non-insulin dependent diabetes mellitus (NIDDM) or type II diabetes. One of the hallmarks of type II diabetes is decreased sensitivity of muscle and adipose cells to insulin. Compounds that increase the sensitivity of adipose to insulin may be useful in the treatment of diabetes and its complications.

Upon insulin treatment, insulin receptor is phosphorylated which activates a signal transduction pathway leading to increased glucose uptake by glucose transporter 4 (GLUT4) in adipocytes (fat) or myocytes (muscle). Therefore, measuring glucose uptake provides the most relevant end point assay for insulin sensitivity.

Our assay uses 3H-2-deoxyglucose as the substrate for glut4. The measurement is conducted in a 96-well format with insulin (100 nM) as a positive control and Cytochalasin B (10µM) as a negative control. Samples are tested in triplicate.

Figure 1 shows glucose uptake after a 2 hour treatment. Data is calculated as mean counts per well. The assay was done in subcutaneous human adipocytes at two weeks post-differentiation.

Ordering Information

Item#	Item Desc	U/M	Price
CA-25	Human Adipocyte Glucose Uptake Assay	Assay	Call

Prices for contract services vary depending on number of sample numbers, special conditions, compound solubility, etc. Minimum charges will apply. Please contact us for detailed information and a price quote on your project.

Recent Publications:	View .PDF
Recent Publications	View .PDF
Subetta Enhances Sensitivity of Human Muscle Cells to Insulin. Gorbunov EA, Nicoll J, Myslivets AA, Kachaeva EV, Tarasov SA. Bull Exp Biol Med. 2015 Aug;159(4):463-5. doi: 10.1007/s10517-015-2992-8. Epub 2015 Sep 21.

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