Lipolysis plays a central role in the regulation of energy balance. Lipolysis is the process in which triglycerides are hydrolyzed into glycerol and free fatty acids. This releases free fatty acids (FFA) into the bloodstream where they may be either re-esterified by the adipocyte or travel to other tissues and exert other effects throughout the body. Elevated adipocyte lipolysis has been observed in obese and diabetic individuals (Arner 1996). Alterations in lipolytic capacity have also been implicated in the susceptibility to obesity of African-American individuals versus their Caucasian cohorts (Danadian et al. 2001)
The breakdown of triglycerides in adipocytes leads to release of free fatty acids and glycerol into the medium. Understanding what causes the breakdown of triglycerides in the cell could have a profound impact on the development of anti-obesity drugs.
ZenBio offers proven assays to detect glycerol and/or non-esterified free fatty acids secreted into the culture medium in response to lipolytic hormone.
Positive Control: 1 µM Isoproterenol Vehicle Control: Appropriate concentration of all solvents
Treatments will be done in triplicate at concentrations indicated by contact scientists. All treatments will be for 3 hours. At the end of treatment period conditioned media will be removed and glycerol concentration determined by GPO-Trinder reagent. Free fatty acid concentration is determined by NEFA reagent.
|CA-30||Lipolysis Assay (glycerol release)||Assay||Call|
|CA-31||Lipolysis Assay (free fatty acid release)||Assay||Call|
|CA-32||Lipolysis Assay (glycerol and FFA release)||Assay||Call|
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