Lipid accumulation assays provide the tools to study the compounds that stimulate cultured human adipocyte differentiation or lipogenesis. Such compounds may be PPAR. agonists or a combination of thiazolidinediones and glucocorticoids that are potentially useful in the treatment of diabetes. Our assays measure potential PPAR agonists or glucocorticoid analogs. Assays include cellular accumulation of lipid after proscribed periods of time in culture, with and without compounds of interest.
Our lipid accumulation assays examine the ability of compounds to stimulate lipid accumulation in the process of differentiating preadipocytes to adipocytes. Cultured preadipocytes will be incubated in the presence of appropriate initiation medium, and test compound. Under these culture conditions and in the presence of the test compound there should be a substantial increase in the amount of lipid accumulation when compared to vehicles. Researchers have a choice between acute and chronic treatments with compound. Researchers must also choose between testing potential PPAR Gamma agonists, or glucocorticoid analogues.
The assay involves complete lysis of the cells. Triglyceride is converted to glycerol and free fatty acids and the glycerol concentration is measured.
- Positive control: PPAR Gamma agonist (10µM) or Dexamethasone (1µM)
- Negative control: PPAR Gamma agonist (10µM) or Dexamethasone (1µM) + TNFa (5ng/ml)
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