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ZenComplete™: Hepatocyte Glucose Production Assay

Diabetes is a prevalent metabolic disease affecting more than 25 million Americans. About 90% to 95% of patients have non-insulin dependent diabetes mellitus (NIDDM) or type II diabetes. One of the hallmarks of type II diabetes is decreased sensitivity of muscle and adipose cells to insulin. Compounds that increase the sensitivity of adipose to insulin may be useful in the treatment of diabetes and its complications.

Upon insulin treatment, insulin receptor is phosphorylated which activates a signal transduction pathway leading to increased glucose uptake by glucose transporter 4 (GLUT4) in adipocytes (fat) or myocytes (muscle). Therefore, measuring glucose uptake provides the most relevant end point assay for insulin sensitivity.

The measurement is conducted in a 96-well format. Samples are tested in triplicate.

Hepatocyte Glucose Production Assay
Figure 1 Cells: primary and hepatoma cell lines
Positive control: Insulin or metformin/phenformin
Vehicle control: Appropriate concentration of solvent for test compounds
Treatments are performed in triplicate at concentrations indicated by contact scientist.

Ordering Information

AssayCat#Price
Human Glucose Productions AssayCA-26Call
Prices for contract services vary depending on number of sample numbers, special conditions, compound solubility, etc. Minimum charges will apply. Please contact us for detailed information and a price quote on your project.

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