Search ZenBio site

ZenComplete™: Protein Secretion

A growing family of adipo-cytokines have been shown to play critical roles in the etiology of obesity and type II diabetes. ZenBio offers an adiponectin assay and a multiplexed assay to measure some of these important adipokines.

Adiponectin Secretion

The development of non-insulin dependent (Type 2) diabetes may involve dysregulation of adiponectin secretion. Supporting the link between obesity and Type 2 diabetes, decreased adiponectin secretion correlates with insulin resistance. Additionally, adiponectin appears to be a potent insulin enhancer linking adipose tissue and whole body glucose metabolism. This assay measures adiponectin in either human serum or conditioned medium from the culture of human cells.

Figure 1 shows several analytes from cultured human preadipocytes, adipocytes, or adipocytes treated with Rosiglitazone. Data are expressed as pg/ml.

Protein Secretion Figure 1
Figure 1 shows adiponectin secretion from human adipocytes in response to increasing concentrations of a PPAR gamma agonist. Two different human adipocyte lots display similar adiponectin secretion responsiveness. Data are expressed as ng/ml.

Adipokine Secretion

The following panel is available: IL-1., Il-6, IL-8, adiponectin, HGF, Leptin, MCP-1, PAI-1 (active), PAI-1 (total), Resistin, NGF and TNF.. Customers may almost any combination of adipokines. All analytes will be measured from the same well. The assay is done in duplicate in cultured human cells.

Protein Secretion Figure 2
Figure 2 shows several analytes from cultured human preadipocytes, adipocytes, or adipocytes treated with Rosiglitazone. Data are expressed as pg/ml.

Ordering Information

Item#Item DescU/MPrice
CA-65Human Adipocyte Adiponectin Secretion AssayAssayCall
CA-110Human Adipocyte Multiplex Adipokine Secretion AssayAssayCall
Prices for contract services vary depending on number of sample numbers, special conditions, compound solubility, etc. Minimum charges will apply. Please contact us for detailed information and a price quote on your project.

To learn more about our services, please contact our Services Team

products
ZenBio is GLP COMPLIANT and CERTIFIED
Products
Services
Technical Support