Rodent (3T3-L1) Glucose Uptake Assay

Diabetes is a prevalent metabolic disease affecting more than 25 million Americans. About 90% to 95% of patients have non-insulin dependent diabetes mellitus (NIDDM) or type II diabetes. One of the hallmarks of type II diabetes is decreased sensitivity of muscle and adipose cells to insulin. Compounds that increase the sensitivity of adipose to insulin may be useful in the treatment of diabetes and its complications.

Glucose uptake assays are performed in 96-well format using mature 3T3-L1 adipocytes. Insulin stimulates [3H]-2-deoxyglucose uptake into the cells, which is quantified by scintillation counting. Assays can be formatted to investigate the stimulation, inhibition, or sensitization of 3T3-L1 adipocyte glucose uptake.

Positive control: 100 nM insulin
Negative control: TNFα (5ng/ml)
Background control: Cytochalasin B (10μM)

Fig. 1 shows [3H]-deoxyglucose uptake in 3T3-L1 adipocytes in response to increasing insulin concentrations.
Fig. 2 shows TNFα inhibition of insulin-stimulate glucose uptake in 3T3-L1 cells. Cells were treated with 50pM TNFα prior to stimulation with 100nM insulin

Ordering Information

AssayCat#Price
Rodent Cell (3T3-L1) Glucose Uptake AssayCA-300Call
Prices for contract services vary depending on number of sample numbers, special conditions, compound solubility, etc. Minimum charges will apply. Please contact us for detailed information and a price quote on your project.
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